Stimulating effect of bombesin on the growth of gastrointestinal tract and pancreas in suckling rats

Lehy, Thérèse; Puccio, François; Chariot, J; Labeille, D.

doi:10.1016/0016-5085(86)90265-9

Cited by 89 publications

(39 citation statements)

References 20 publications

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“…Results of the present investigation confirm these data and the previous ones from other [15][16][17] and our [22] laboratories showing that bombesin has a growth-promoting effect on the rat pancreas. They additionally present evidence that after short-term (5 days) bombesin treatment, the secretory ca- pacity (both in basal conditions and after caerulein stimulation) of the exocrine pan creas is strongly increased.…”

Section: Discussionsupporting

confidence: 83%

Effect of Bombesin and Its Mammalian Counterpart, GRP, on Exocrine Pancreas in the Rat

et al. 1988

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The effect of equimolar doses (6 nmol/kg) of bombesin and its mammalian counterpart, GRP, on pancreatic growth and secretion was studied in adult rats. Both peptides were administered intraperitoneally three times a day for 5 consecutive days. Saline-treated rats were used as controls. At the end of the treatment, animals were anaesthetized and pancreatic juice was collected in basal conditions and after caerulein (0.75 nmol/kg i.p.) stimulation. Afterwards, the rats were sacrificed and growth and composition of the pancreatic tissue were determined. Compared with the control (saline) values, either basal or stimulated secretion was significantly increased after short-term treatment with both peptides. In addition, both bombesin and GRP increased pancreatic weight, total pancreatic protein, trypsin and amylase content. The DNA content was also increased by both peptides, although only the GRP effect proved to be significant. These results demonstrate that both bombesin and GRP have a growth-promoting effect on rat pancreas and concomitantly increase its secretory capacity. The mechanism of this peculiar biological action is likely to be connected with a direct stimulatory action on the gland.

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Section: Discussionsupporting

confidence: 83%

Effect of Bombesin and Its Mammalian Counterpart, GRP, on Exocrine Pancreas in the Rat

et al. 1988

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“…Previous studies in rats reported that bombesin exerts some trophic effects on the pancreas (Lhoste et al 1985;Lehy et al 1986), but our study with …”

Section: Discussioncontrasting

confidence: 49%

Cholecystokinin receptor antagonism by peptidergic and non‐peptidergic agents in rat pancreas.

Dembiński¹,

Jaworek²,

Konturek³

et al. 1989

The Journal of Physiology

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SUMMARY1. Graded doses of bombesin infused I.v. into conscious rats with chronic pancreatic fistulae induced a dose-dependent stimulation of protein secretion, similar to that obtained with caerulein. This stimulation does not appear to be mediated by cholecystokinin (CCK) receptors because peptidergic (CR-1409) and non-peptidergic (L-364 718) CCK antagonists failed to affect protein secretion at a dose range which caused almost complete suppression of caerulein-induced pancreatic secretion.2. Studies in vitro on isolated rat pancreatic acini revealed that caerulein, pentagastrin and bombesin all showed the same efficacy in their ability to stimulate amylase release. In contrast, CCK antagonists competitively inhibited amylase release induced by caerulein and pentagastrin but not by bombesin or urecholine, indicating that the latter two agents act directly on acinar cells via receptors which are separate from those involved in stimulation induced by caerulein and pentagastrin.3. DNA synthesis, measured by the incorporation of [3H]thymidine into DNA, was significantly stimulated by caerulein, soybean trypsin inhibitor (FOY 305), pentagastrin and by bombesin in a dose-dependent manner. CCK receptor antagonists prevented stimulation of DNA synthesis induced by caerulein, FOY 305 and pentagastrin but not by bombesin.4. This study indicates that bombesin strongly stimulates pancreatic enzyme secretion, with an efficacy similar to that of caerulein, and also exerts a potent growth-promoting action on the pancreas, both effects appearing to be mediated by mechanisms independent of the CCK receptors.

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“…Additionally, Cuttitta et al (15) have shown that an antibody to the GRP receptor blocks the growth of SCCL both in vitro and in vivo. Recent studies have also shown that GRP stimulates pancreatic and intestinal growth (25,26).…”

Section: Introductionmentioning

confidence: 99%

Transient elevation of messenger RNA encoding gastrin-releasing peptide, a putative pulmonary growth factor in human fetal lung.

Spindel¹,

Sunday²,

Höfler³

et al. 1987

J. Clin. Invest.

125

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Gastrin-releasing peptide (GRP), the mammalian homologue of the amphibian peptide bombesin, is present in pulmonary neuroendocrine cells and appears to be a growth factor for both normal and neoplastic pulmonary cells. Previously we have reported the cloning of the messenger RNAs (mRNAs) and gene that encode human GRP. We now report that GRP mRNAs are markedly elevated in human fetal lung during the canalicular phase of pulmonary development (from -16 to 30 wk gestation). By RNA blot and in situ hybridization analyses, GRP mRNAs were first detectable in fetal lung at 9-10 wk, plateaued at levels 25-fold higher than in adult lungs from 16 to -30 wk and then declined to near adult levels by 34 wk gestation. By contrast, GRP peptide levels remain elevated until several months after birth. Consistent with this, in situ hybridization and immunohistochemical studies showed that GRP mRNA and peptide consistently colocalized in early gestation lung but that in neonatal lung, many cells that contained GRP peptide no longer contained GRP mRNA. The transient expression of high levels of GRP mRNAs during an -12-wk phase of fetal lung development suggests that the secretion of GRP or its COOH-terminal peptides from pulmonary neuroendocrine cells may play a role in normal lung development.

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Stimulating effect of bombesin on the growth of gastrointestinal tract and pancreas in suckling rats

Cited by 89 publications

References 20 publications

Effect of Bombesin and Its Mammalian Counterpart, GRP, on Exocrine Pancreas in the Rat

Effect of Bombesin and Its Mammalian Counterpart, GRP, on Exocrine Pancreas in the Rat

Cholecystokinin receptor antagonism by peptidergic and non‐peptidergic agents in rat pancreas.

Transient elevation of messenger RNA encoding gastrin-releasing peptide, a putative pulmonary growth factor in human fetal lung.

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