Stimulation of oral mucosal regeneration by low intensity pulsed ultrasound: an in vivo study in a porcine model

Chauvel-Picard, J.; Korn, Philippe; Corbin, Sara; Brosset, Sophie; Béra, J.-C.; Gleizal, A.

doi:10.2186/jpr.jpor_2019_345

Cited by 5 publications

(7 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Low-intensity pulsed ultrasound (LIPUS), a form of ultrasound delivers at a low intensity and outputs in the mode of pulsed waves, has been demonstrated as adjuvant physical therapy, including promoting the differentiation of stem cells [ 22 , 23 ], inhibiting inflammatory responses [ 24 ], accelerating soft tissue regeneration [ 25 , 26 ], modulating neuronal activity [ 27 , 28 ], improving bone healing [ 29 , 30 ], and cartilage regeneration [ 31 – 35 ]. In this study, we investigated the promoting effects and mechanisms underlying LIPUS stimulation on the chondrogenic differentiation of human umbilical cord mesenchymal stem cells (hUC-MSCs) and further evaluated its regenerative application value in articular cartilage defects in rats.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, we verified the safety of LIPUS stimulation and hUC-MSC transplantation-based cartilage repairing procedure. LIPUS non-invasively delivers mechanical stimulation to biological tissues without thermal activity and has been extensively exploited as a therapeutic tool in tissue engineering research, including cartilage regeneration [ 31 – 35 ]. However, it remains vital to determine and validate the LIPUS parameters for its safe and effective use.…”

Section: Discussionmentioning

confidence: 99%

“…As an adjuvant physical therapy, LIPUS has been demonstrated to promote the differentiation of stem cells [ 22 , 23 ], inhibit inflammatory responses [ 24 ], accelerate soft tissue regeneration [ 25 , 26 ], modulate neuronal activity [ 27 , 28 ], and improve bone healing [ 29 , 30 ]. In particular, LIPUS has been extensively exploited in tissue engineering research, including cartilage regeneration [ 31 – 35 ].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Low-intensity pulsed ultrasound promotes mesenchymal stem cell transplantation-based articular cartilage regeneration via inhibiting the TNF signaling pathway

et al. 2023

View full text Add to dashboard Cite

Background Mesenchymal stem cell (MSC) transplantation therapy is highly investigated for the regenerative repair of cartilage defects. Low-intensity pulsed ultrasound (LIPUS) has the potential to promote chondrogenic differentiation of MSCs. However, its underlying mechanism remains unclear. Here, we investigated the promoting effects and mechanisms underlying LIPUS stimulation on the chondrogenic differentiation of human umbilical cord mesenchymal stem cells (hUC-MSCs) and further evaluated its regenerative application value in articular cartilage defects in rats. Methods LIPUS was applied to stimulate cultured hUC-MSCs and C28/I2 cells in vitro. Immunofluorescence staining, qPCR analysis, and transcriptome sequencing were used to detect mature cartilage-related markers of gene and protein expression for a comprehensive evaluation of differentiation. Injured articular cartilage rat models were established for further hUC-MSC transplantation and LIPUS stimulation in vivo. Histopathology and H&E staining were used to evaluate the repair effects of the injured articular cartilage with LIPUS stimulation. Results The results showed that LIPUS stimulation with specific parameters effectively promoted the expression of mature cartilage-related genes and proteins, inhibited TNF-α gene expression in hUC-MSCs, and exhibited anti-inflammation in C28/I2 cells. In addition, the articular cartilage defects of rats were significantly repaired after hUC-MSC transplantation and LIPUS stimulation. Conclusions Taken together, LIPUS stimulation could realize articular cartilage regeneration based on hUC-MSC transplantation due to the inhibition of the TNF signaling pathway, which is of clinical value for the relief of osteoarthritis.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Low-intensity pulsed ultrasound promotes mesenchymal stem cell transplantation-based articular cartilage regeneration via inhibiting the TNF signaling pathway

et al. 2023

View full text Add to dashboard Cite

show abstract

“…Low-intensity ultrasound had a similar effect on the activation of connective tissue cells, such as human osteoblast-like cell lines and HPDLCs (Jiang et al, 2019). There is also evidence from a recent study on a porcine model that the application of LIPUS on the oral mucosa expedites the repair of the masticatory mucosa (Chauvel-Picard et al, 2021). However, soft-tissue healing remains less studied than bone healing area.…”

Section: Soft-tissue Regenerationmentioning

confidence: 99%

LIPUS as a potential strategy for periodontitis treatment: A review of the mechanisms

Aimaijiang

Liu²,

Zhang³

et al. 2023

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Periodontitis is a chronic inflammatory condition triggered by oral bacteria. A sustained inflammatory state in periodontitis could eventually destroy the alveolar bone. The key objective of periodontal therapy is to terminate the inflammatory process and reconstruct the periodontal tissues. The traditional Guided tissue regeneration (GTR) procedure has unstable results due to multiple factors such as the inflammatory environment, the immune response caused by the implant, and the operator’s technique. Low-intensity pulsed ultrasound (LIPUS), as acoustic energy, transmits the mechanical signals to the target tissue to provide non-invasive physical stimulation. LIPUS has positive effects in promoting bone regeneration, soft-tissue regeneration, inflammation inhibition, and neuromodulation. LIPUS can maintain and regenerate alveolar bone during an inflammatory state by suppressing the expression of inflammatory factors. LIPUS also affects the cellular behavior of periodontal ligament cells (PDLCs), thereby protecting the regenerative potential of bone tissue in an inflammatory state. However, the underlying mechanisms of the LIPUS therapy are still yet to be summarized. The goal of this review is to outline the potential cellular and molecular mechanisms of periodontitis-related LIPUS therapy, as well as to explain how LIPUS manages to transmit mechanical stimulation into the signaling pathway to achieve inflammatory control and periodontal bone regeneration.

show abstract

“…The interest in LIPUS appeared about two decades ago with research on their use in biological media. Many studies have shown the ability of LIPUS to stimulate bone, cartilage, tendon and mucosal regeneration [11][12][13][14][15]. At the cellular level, ultrasound promotes the synthesis of collagen by fibroblasts, the formation of bone matrix by osteoblasts, the differentiation of bone mesenchymal stem cells (BMSC) in osteoblasts on titanium surfaces and the synthesis of aggrecan in chondrocytes.…”

Section: Introductionmentioning

confidence: 99%

Stimulation of dental implant osseointegration by low-Intensity pulsed ultrasound: An <i>in vivo</i> preliminary study in a porcine model

Chauvel-Picard¹,

Gourmet

Vercherin³

et al. 2022

J Prosthodont Res

Self Cite

View full text Add to dashboard Cite

Purposes: Several studies have evaluated the interest of Low Intensity Pulsed Ultrasound (LIPUS) in the osseointegration of dental implants in murine or rabbit models. However, the thinness and narrowness bones make it difficult to study the effect of LIPUS. The purpose of this study is to assess the ability of LIPUS to stimulate bone formation in contact with a titanium dental implant in a porcine model. Methods: Eight adults mini-pigs were used. An implant is placed on each tibial crest in the metaphysis. The right side was treated with LIPUS at 1 MHz and 300 mW/cm 2 of acoustic intensity during 15 minutes per day on 5 consecutive days and during 42 days. The left side was not treated. The Bone Volume/Total Volume ratio (BV/TV), the Intersection Surface (IS) of the volume of interest by the binarized bone and the Trabecular bone Thickness (TbTh) around the implant were analyzed. Results: At 42 days, BV/TV ratio is significantly higher on the treated side (42,1+/−8,76% versus 32,31+/−10,11%, p < 0,02); as well as TbTh with 0,13+/−0,01 mm versus 0,10+/−0,01 mm (p < 0,01). IS is also significantly higher on the treated side (40,7 +/− 12,68 mm 2 versus 33,68+/−9,44 mm 2 at 200 μm from the implant surface; p < 0,01). Conclusion:The present study showed that LIPUS can significantly increase bone formation and accelerate the healing process at the bone-implant interface in a porcine model. Its low toxicity, low immunogenicity and non-invasion make it a complementary treatment of choice for improving the bone formation around titanium implants.

show abstract

Stimulation of oral mucosal regeneration by low intensity pulsed ultrasound: an in vivo study in a porcine model

Cited by 5 publications

References 32 publications

Low-intensity pulsed ultrasound promotes mesenchymal stem cell transplantation-based articular cartilage regeneration via inhibiting the TNF signaling pathway

Low-intensity pulsed ultrasound promotes mesenchymal stem cell transplantation-based articular cartilage regeneration via inhibiting the TNF signaling pathway

LIPUS as a potential strategy for periodontitis treatment: A review of the mechanisms

Stimulation of dental implant osseointegration by low-Intensity pulsed ultrasound: An <i>in vivo</i> preliminary study in a porcine model

Contact Info

Product

Resources

About