Stimulation of Spermatogonial Differentiation in Juvenile Spermatogonial Depletion (<i>jsd</i>) Mutant Mice by Gonadotropin-Releasing Hormone Antagonist Treatment

Matsumiya, Kiyomi; Meistrich, Marvin L.; Shetty, Gunapala; Dohmae, Kayoko; Tohda, Akira; Okuyama, Äkïhïko; Nishimune, Yoshitake

doi:10.1210/endo.140.10.7026

Cited by 47 publications

(41 citation statements)

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“…Strong AR immunoreactivity was also observed in interstitial cells already on the first postnatal day and in Sertoli cells from 21 d onward, parallel to findings in other rodents (Kimura et al, 1993;Bremner et al, 1994;Zhou et al, 1996). The lack of AR immunoreaction for spermatogonial subtypes is also consistent with previous reports (Bremner et al, 1994;Vornberger et al, 1994;Matsumiya et al, 1999). AR functions as a ligand-dependent transcription factor, since ligand binding is a prerequisite for its transport to the nucleus where it accesses and regulates an array of androgen-responsive genes (Georget et al, 1997;Wang et al, 2009).…”

Section: Discussionsupporting

confidence: 90%

Neonatal Gonocyte Differentiation in Mongolian GerbilMeriones unguiculatusInvolves Asynchronous Maturation of Seminiferous Cords and Rapid Formation of Transitional Cell Stage

Pinto

Botta

Taboga

et al. 2010

The Anatomical Record

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This study describes the neonatal differentiation of the Mongolian gerbil gonocytes, focusing on the relationship between its relocation to the basement membrane, apoptosis and postrelocation changes and also the distribution of androgen receptors (AR). Testes of gerbils from 1 to 35 days of age (d) were examined by high resolution light microscopy and immunocytochemistry for proteins PCNA, VASA, and AR as well as by the TUNEL method. Gonocytes were quantified according to degree of relocation into nonrelocated, relocating and relocated. Most of them were found in the center of seminiferous cords at 1 d but a small number of relocating and relocated gonocytes were already visible in the first postnatal day. After relocation, gonocytes change phenotypically to a transitional stage designated herein prospermatogonia. Both gonocyte relocation and transformation into spermatogonial lineage occur asynchronously in the seminiferous cords, mainly after 7 d. Gonocyte proliferation began before but peak after their relocation to basement membrane at the prospermatogonia stage. Higher levels of gonocyte apoptosis were found at 7 d and 21 d. From this time onward gonocytes were not found. Gonocytes and prospermatogonia showed high amounts of AR in their cytoplasm contrary to spermatogonial subtypes, indicating a possible AR inactivation in these cells. In conclusion, the process of gonocyte relocation in the gerbil extends until the second postnatal week, leads to their rapid differentiation into prospermatogonia and occurs simultaneously with the loss of androgen sensitivity. Differently from other laboratory rodents, the events regarding gonocyte maturation in the gerbil last longer and occur asynchronously in seminiferous cords. Anat Rec,

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Section: Discussionsupporting

confidence: 90%

Neonatal Gonocyte Differentiation in Mongolian GerbilMeriones unguiculatusInvolves Asynchronous Maturation of Seminiferous Cords and Rapid Formation of Transitional Cell Stage

Pinto

Botta

Taboga

et al. 2010

The Anatomical Record

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“…Testicular homogenates were prepared following the method of Matsumiya et al (26) to obtain intratesticular measurements of estradiol. The left testis was weighed, homogenized in 0.5 ml of water, and the sample was centrifuged at 10,000 rpm for 10 min.…”

Section: Methodsmentioning

confidence: 99%

Aromatase ( Cyp19 ) expression is up-regulated by targeted disruption of Dax1

Wang

Jeffs²,

Ito³

et al. 2001

Proc. Natl. Acad. Sci. U.S.A.

127

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DAX-1 [dosage-sensitive sex reversal, adrenal hypoplasia congenita (AHC) critical region on the X chromosome, gene 1] is an orphan nuclear receptor that represses transcription by steroidogenic factor-1 (SF-1), a factor that regulates expression of multiple steroidogenic enzymes and other genes involved in reproduction. Mutations in the human DAX1 gene (also known as AHC) cause the X-linked syndrome AHC, a disorder that is associated with hypogonadotropic hypogonadism also. Characterization of Dax1-deficient male mice revealed primary testicular defects that included Leydig cell hyperplasia (LCH) and progressive degeneration of the germinal epithelium, leading to infertility. In this study, we investigated the effect of Dax1 disruption on the expression profile of various steroidogenic enzyme genes in Leydig cells isolated from Dax1-deficient male mice. Expression of the aromatase (Cyp19) gene, which encodes the enzyme that converts testosterone to estradiol, was increased significantly in the Leydig cells isolated from mutant mice, whereas the expression of other proteins (e.g., StAR and Cyp11a) was not altered. In in vitro transfection studies, DAX-1 repressed the SF-1-mediated transactivation of the Cyp19 promoter but did not inhibit the StAR or Cyp11a promoters. Elevated Cyp19 expression was accompanied by increased intratesticular levels of estradiol. Administration of tamoxifen, a selective estrogen-receptor modulator, restored fertility to the Dax1-deficient male mice and partially corrected LCH, suggesting that estrogen excess contributes to LCH and infertility. Based on these in vivo and in vitro analyses, aromatase seems to be a physiologic target of Dax-1 in Leydig cells, and increased Cyp19 expression may account, in part, for the infertility and LCH in Dax1-deficient mice.

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“…Spermatogonia undergo meiosis and spermiogenesis subsequently in the juvenile testis environment that is endocrinologically different from that of the adult testis (Matsumiya et al, 1999). However, many of them die at spermatocyte-stage because of an increase in apoptosis mediated by Caspase3 and Bcl-2 family proteins (Billing el al., 1995;Furuchi et al, 1996;Jahnukainen et al, 2003).…”

Section: Introductionmentioning

confidence: 99%