Stimulus-dependent dissociation between XB130 and Tks5 scaffold proteins promotes airway epithelial cell migration

Moodley, Serisha; Derouet, Mathieu; Bai, Xiao Liang; Xu, Feng; Kapùs, András; Yang, Burton B.; Liu, Mingyao

doi:10.18632/oncotarget.13261

Cited by 8 publications

(6 citation statements)

References 37 publications

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“…By contrast, BEAS-2B cells showed cuboidal shape (Figure 1D), and surface marker E-cadherin was detected by Western blotting (Figure 1E). XB130, an adaptor protein that is involved in the regulation of cell proliferation and motility in BEAS-2B cells [23][24][25], was only observed in lung epithelial cells (Figure 1E), whereas vWF and PECAM1 were not detected from epithelial cells (Figure 1D-F).…”

Section: Cold Preservation and Warm Reperfusion Reduced Viability Of Hpmec And Beas-2b Cellsmentioning

confidence: 99%

Ischemia-Reperfusion Injury in a Simulated Lung Transplant Setting Differentially Regulates Transcriptomic Profiles between Human Lung Endothelial and Epithelial Cells

Saren

Wong

et al. 2021

Cells

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Current understanding of mechanisms of ischemia-reperfusion-induced lung injury during lung preservation and transplantation is mainly based on clinical observations and animal studies. Herein, we used cell and systems biology approaches to explore these mechanisms at transcriptomics levels, especially by focusing on the differences between human lung endothelial and epithelial cells, which are crucial for maintaining essential lung structure and function. Human pulmonary microvascular endothelial cells and human lung epithelial cells were cultured to confluent, subjected to different cold ischemic times (CIT) to mimic static cold storage with preservation solution, and then subjected to warm reperfusion with a serum containing culture medium to simulate lung transplantation. Cell morphology, viability, and transcriptomic profiles were studied. Ischemia-reperfusion injury induced a CIT time-dependent cell death, which was associated with dramatic changes in gene expression. Under normal control conditions, endothelial cells showed gene clusters enriched in the vascular process and inflammation, while epithelial cells showed gene clusters enriched in protein biosynthesis and metabolism. CIT 6 h alone or after reperfusion had little effect on these phenotypic characteristics. After CIT 18 h, protein-biosynthesis-related gene clusters disappeared in epithelial cells; after reperfusion, metabolism-related gene clusters in epithelial cells and multiple gene clusters in the endothelial cells also disappeared. Human pulmonary endothelial and epithelial cells have distinct phenotypic transcriptomic signatures. Severe cellular injury reduces these gene expression signatures in a cell-type-dependent manner. Therapeutics that preserve these transcriptomic signatures may represent new treatment to prevent acute lung injury during lung transplantation.

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Section: Cold Preservation and Warm Reperfusion Reduced Viability Of Hpmec And Beas-2b Cellsmentioning

confidence: 99%

Ischemia-Reperfusion Injury in a Simulated Lung Transplant Setting Differentially Regulates Transcriptomic Profiles between Human Lung Endothelial and Epithelial Cells

Saren

Wong

et al. 2021

Cells

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“…More recently, an interaction between the fifth SH3 domain of Tks5 and the actinbinding protein XB130 was reported. Interference with this interaction affected cell survival and proliferation of bronchial epithelial cells (Moodley et al, 2015), as well as cell migration (Moodley et al, 2016). Whether this interaction impacts invadosome formation remains to be tested.…”

Section: The Interactome Of Tks Adaptor Proteinsmentioning

confidence: 99%

Tks adaptor proteins at a glance

Saini

Courtneidge

2018

Journal of Cell Science

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Tyrosine kinase substrate (Tks) adaptor proteins are considered important regulators of various physiological and/or pathological processes, particularly cell migration and invasion, and cancer progression. These proteins contain PX and SH3 domains, and act as scaffolds, bringing membrane and cellular components in close proximity in structures known as invadopodia or podosomes. Tks proteins, analogous to the related proteins p47, p40 and NoxO1, also facilitate local generation of reactive oxygen species (ROS), which aid in signaling at invadopodia and/or podosomes to promote their activity. As their name suggests, Tks adaptor proteins are substrates for tyrosine kinases, especially Src. In this Cell Science at a Glance article and accompanying poster, we discuss the known structural and functional aspects of Tks adaptor proteins. As the science of Tks proteins is evolving, this article will point out where we stand and what still needs to be explored. We also underscore pathological conditions involving these proteins, providing a basis for future research to develop therapies for treatment of these diseases.

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“…For instance, as a substrate and mediator of multiple tyrosine kinases, XB130 can be phosphorylated and binds to the p85a subunit of phosphatidylinositol-3-kinase (PI3K), activating PI3K/Akt pathway [12,13]. The interaction between XB130 and scaffold protein Tks5 regulates Src-mediated cell proliferation [14,15]. And there is XB130/Rac interation-dependent cell motility [16].…”

Section: Discussionmentioning

confidence: 99%

The Expression and Significance of XB130 in Skin Basal Cell Carcinoma

Huang¹,

Duan²,

Wang³

et al. 2019

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Background: Skin basal cell carcinoma (BCC) is the most common skin cancer with an increasing incidence rate worldwide. XB130 is a novel adaptor protein for intracellular signal transduction and it participates in cell proliferation, cell survival, cell migration and cell apoptosis. Studies have demonstrated that XB130 is involved in cancer initiation and progression; however, its expression and role in BCC have not been investigated. Methods: Immunohistochemistry was carried out to detect the expression of XB130 at protein level in skin samples obtained from patients with skin basal cell carcinoma (n=40) and healthy donors (n=35). Results: In our study, we found that the staining of XB130 was mostly observed in cytoplasm. In normal skin tissues, XB130 was expressed almost in the whole layer of the epidermis. And the expression of XB130 in BCC was significantly down-regulated compared to normal skin. Conclusions: The study suggested that XB130 is involved in the pathogenesis of BCC and it may serve as a therapeutic target of BCC. Keywords: XB130; skin basal cell carcinoma;

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Stimulus-dependent dissociation between XB130 and Tks5 scaffold proteins promotes airway epithelial cell migration

Cited by 8 publications

References 37 publications

Ischemia-Reperfusion Injury in a Simulated Lung Transplant Setting Differentially Regulates Transcriptomic Profiles between Human Lung Endothelial and Epithelial Cells

Ischemia-Reperfusion Injury in a Simulated Lung Transplant Setting Differentially Regulates Transcriptomic Profiles between Human Lung Endothelial and Epithelial Cells

Tks adaptor proteins at a glance

The Expression and Significance of XB130 in Skin Basal Cell Carcinoma

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