Stirred Bioreactors

Jossen, Valentin; Eibl-Schindler, Regine; Pörtner, Ralf; Kraume, Matthias; Eibl, Dieter

doi:10.1016/b978-0-444-63663-8.00007-0

Cited by 19 publications

(23 citation statements)

References 95 publications

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“…4a) were calculated for 2xRT, and 19.8 kW m -3 (Ne = 13.4) and 12.5 kW m -3 (Ne = 12.1), respectively, for 3xRT. These values are within the required range for microbial processes [4]. Experimentally determined mixing times decreased with increasing power input and showed the lowest values within a range of 15 to 8 s (30 L), 21 to 10 s (100 L) for 2xRT ( Fig.…”

Section: Resultssupporting

confidence: 70%

“…The values for specific power input and k L a are within the typical range for microbial bioreactors of this size [4,13]. The values for specific power input and k L a are within the typical range for microbial bioreactors of this size [4,13].…”

Section: Discussionsupporting

confidence: 59%

“…The process engineering parameters of the D-DCU 30 L and D-DCU 100 L allow to define the optimum operational ranges and limits of the bioreactors. The values for specific power input and k L a are within the typical range for microbial bioreactors of this size [4,13]. Interestingly, a comparison of the obtained biomass and acetate concentrations at the end of the batch cultivations revealed differences in bioreactor performance, which could not be detected during process engineering characterization.…”

Section: Discussionmentioning

confidence: 51%

“…Users can select the most suitable version for their application from a broad variety of manufacturers. The performance capabilities of the bioreactor, which are substantially influenced by its design and thus, its bioengineering characteristics, are crucial in order to choose a system matching the specific process requirements [4,6]. Knowledge about mixing times, volumetric mass transfer coefficients (k L a values), and the specific power input supports the definition of suitable process parameters, e.g., stirrer tip speed and aeration rate, needed to reach the desired product quantity and quality, as well as for subsequent scale-up [7].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Standardized Qualification of Stirred Bioreactors for Microbial Biopharmaceutical Production Processes

Schirmer

Blaschczok

Husemann

et al. 2017

Chemie Ingenieur Technik

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An in 2016 published DECHEMA guideline concerning process engineering characterization and a new Escherichia coli model process were utilized for the qualification of two geometrically similar stirred stainless steel bioreactors (30 L and 100 L working volume). The achieved results demonstrate that performing an additional biological model process is a valuable complement to the process engineering characterization. Optical densities of 27 (100 L) and 39 (30 L) were reached in the batch cultivation process.

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Section: Resultssupporting

confidence: 70%

Section: Discussionsupporting

confidence: 59%

Section: Discussionmentioning

confidence: 51%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Standardized Qualification of Stirred Bioreactors for Microbial Biopharmaceutical Production Processes

Schirmer

Blaschczok

Husemann

et al. 2017

Chemie Ingenieur Technik

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“…In a stirred tank reactor (STR) an impeller is used for mixing. STRs are also available as single‐use systems and have become widely accepted for product expression . Aeration is usually realized by a ringsparger close to the bottom of the reactor.…”

Section: Introductionmentioning

confidence: 99%

Polysialic acid production using Escherichia coli K1 in a disposable bag reactor

Vries

Busse

Kopatz

et al. 2017

Engineering in Life Sciences

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Polysialic acid (polySia), consisting of α-(2,8)-linked N-acetylneuraminic acid monomers plays a crucial role in many biological processes. This study presents a novel process for the production of endogenous polySia using Escherichia coli K1 in a disposable bag reactor with wave-induced mixing.Disposable bag reactors provide easy and fast production in terms of regulatory requirements as GMP, flexibility and can easily be adjusted to larger production capacities not only by scale up but also by parallelization. Due to the poor oxygen transfer rate compared to a stirred tank reactor (STR), pure oxygen was added during the cultivation to avoid oxygen limitation. During the exponential growth phase the growth rate was 0.61 h -1 . Investigation of stress-related product release from the cell surface showed no significant differences between the disposable bag reactor with wave-induced mixing and the STR. After batch cultivation a cell dry weight (CDW) of 6.8 g L -1 and a polySia concentration of www.els-journal.com Page 2 Engineering in Life SciencesThis article is protected by copyright. All rights reserved.

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Heat and Mass Transfer at the Wall of a Square Mechanically Stirred Gas‐Liquid‐Solid Catalytic Reactor

et al. 2021

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Wall mass and heat transfer rates in a square gas‐sparged, mechanically stirred reactor were measured by the electrochemical technique under the effect of various geometrical and hydrodynamic variables. For the 45° impeller, the mass transfer data fit the equation Sh = 0.7Sc0.33Re0.2Reg0.5 with an average deviation of ±6.9 %. For the 90° impeller, the data fit the equation Sh = 0.95Sc0.33Re0.14Reg0.53 with an average deviation of ±7.5 %. Gas sparging enhanced the wall mass transfer rates by factors of up to 2.61 and 3 for the 90° and 45° impellers, respectively, with a significant decrease in the total power consumption. The contribution of the present results to the operation of multiphase mechanically agitated vessels in different aspects is outlined.

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Stirred Bioreactors

Cited by 19 publications

References 95 publications

Standardized Qualification of Stirred Bioreactors for Microbial Biopharmaceutical Production Processes

Standardized Qualification of Stirred Bioreactors for Microbial Biopharmaceutical Production Processes

Polysialic acid production using Escherichia coli K1 in a disposable bag reactor

Heat and Mass Transfer at the Wall of a Square Mechanically Stirred Gas‐Liquid‐Solid Catalytic Reactor

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