StMBF1c positively regulates disease resistance to Ralstonia solanacearum via it’s primary and secondary upregulation combining expression of StTPS5 and resistance marker genes in potato

Yu, Rui-Min; Suo, Yanyun; Yang, Rui; Chang, Yannan; Tian, Tian; Song, Yun‐Chun; Wang, Huanjun; Yang, Ru-Jie; Liu, Hongliang; Gao, Gang

doi:10.1016/j.plantsci.2021.110877

Cited by 8 publications

(7 citation statements)

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“…ZaMBF1c was observed to be more sensitive to these treatment and exhibited significant resistance, even after 3 days. In fact, MBF1c is a vital member that responds to the environmental changes in various species [ 19 , 22 , 49 ] and can interact with TPS gene family to defend against environmental stress [ 52 ]. Moreover, the function of MBF1 genes has been of great interest in many plants [ 18 , 19 , 22 , 53 , 54 ], but they appeared to have different roles in the responsiveness to cold stress [ 14 ].…”

Section: Discussionmentioning

confidence: 99%

“…Future studies should focus on generating transgenic Zanthoxylum lines to reveal the molecular functions of ZaMBF1 genes [ 22 ]. The proteins that interact with ZaMBF1 could be explored by yeast two hybridization, bimolecular fluorescence complementarity and co-immunoprecipitation assay technique [ 52 ]. Promoter identification could also be conducted to uncover the genetic information of ZaMBF1c related to floral meristem differentiation in Z .…”

Section: Discussionmentioning

confidence: 99%

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Genome-wide characterization of the MBF1 gene family and its expression pattern in different tissues and stresses in Zanthoxylum armatum

et al. 2022

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Background Multiprotein bridging factor 1 (MBF1) is a crucial transcriptional coactivator in animals, plants, and some microorganisms, that plays a necessary role in growth development and stress tolerance. Zanthoxylum armatum is an important perennial plant for the condiments and pharmaceutical industries, whereas the potential information in the genes related to stress resistance remains poorly understood in Z. armatum. Results Herein, six representative species were selected for use in a genome-wide investigation of the MBF1 family, including Arabidopsis thaliana, Oryza sativa, Populus trichocarpa, Citrus sinensis, Ginkgo biloba, and Z. armatum. The results showed that the MBF1 genes could be divided into two groups: Group I contained the MBF1a and MBF1b subfamilies, and group II was independent of the MBF1c subfamily.. Most species have at least two different MBF1 genes, and MBF1c is usually an essential member. The three ZaMBF1 genes were respectively located on ZaChr26, ZaChr32, and ZaChr4 of Zanthoxylum chromosomes. The collinearity were occurred between three ZaMBF1 genes, and ZaMBF1c showed the collinearity between Z. armatum and both P. trichocarpa and C. sinensis. Moreover, many cis-elements associated with abiotic stress and phytohormone pathways were detected in the promoter regions of MBF1 of six representative species. The ERF binding sites were the most abundant targets in the sequences of the ZaMBF1 family, and some transcription factor sites related to floral differentiation were also identified in ZaMBF1c, such as MADS, LFY, Dof, and AP2. ZaMBF1a was observed to be very highly expressed in 25 different samples except in the seeds, and ZaMBF1c may be associated with the male and female floral initiation processes. In addition, expression in all the ZaMBF1 genes could be significantly induced by water-logging, cold stress, ethephon, methyl jasmonate, and salicylic acid treatments, especially in ZaMBF1c. Conclusion The present study carried out a comprehensive bioinformatic investigation related to the MBF1 family in six representative species, and the responsiveness of ZaMBF1 genes to various abiotic stresses and phytohormone inductions was also revealed. This work not only lays a solid foundation to uncover the biological roles of the ZaMBF1 family in Z. armatum, but also provides some broad references for conducting the MBF1 research in other plants.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Genome-wide characterization of the MBF1 gene family and its expression pattern in different tissues and stresses in Zanthoxylum armatum

et al. 2022

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“…In addition, salt, drought, trehalose, salicylic acid, methyl viologen, abscisic acid, and other stresses and hormone treatments can increase the expression of AtMBF1c [ 19 , 20 ]. MBF1c is induced by salicylic acid (SA), methyl jasmonate (MJ), abscisic acid, and Ralstonia solanacearum , and overexpression of MBF1c results in improved resistance to R. solanacearum in S. tuberosum [ 21 ]. However, whether MBF1c participates in waterlogging tolerance has not been reported.…”

Section: Discussionmentioning

confidence: 99%

The transcriptional coactivator CmMBF1c is required for waterlogging tolerance in Chrysanthemum morifolium

Zhao

Yan

et al. 2022

Horticulture Research

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Waterlogging is one of the most serious abiotic stressors affecting Chrysanthemum morifolium during its lifespan. However, the molecular mechanisms underlying the waterlogging tolerance of chrysanthemum remain unclear. In this study, we discovered that the transcriptional coactivator MULTIPROTEIN BRIDGING FACTOR 1c (CmMBF1c) was significantly induced by waterlogging stress in chrysanthemums. Promoter sequence analysis and transient dual-luciferase assay using chrysanthemum protoplasts showed that the waterlogging-tolerant cultivar ‘Nannongxuefeng’ carried more response elements involved in waterlogging and hypoxia stress compared with the waterlogging-sensitive cultivar ‘Qinglu’, conferring ‘Nannongxuefeng’ a stronger hypoxia responsive activity and higher CmMBF1c expression under waterlogging conditions. Subcellular localization and transcriptional activity assays showed that CmMBF1c protein was localized to the nucleus and had no transcriptional activation activity. Overexpression of CmMBF1c in ‘Qinglu’ enhanced its waterlogging tolerance by promoting its ROS scavenging ability and maintaining low reactive oxygen species (ROS) levels. However, RNAi-mediated knockdown of CmMBF1c in cultivar ‘Nannongxuefeng’ resulted in the opposite tendency. Yeast two-hybrid screening and tobacco bimolecular fluorescence complementation (BiFC) assays revealed that CmHRE2, a pivotal regulator of hypoxia response, could interact with CmMBF1c. In summary, this study demonstrates that CmMBF1c improves chrysanthemum waterlogging tolerance by regulating its ROS signaling pathway and interacting with CmHRE2. These findings together offer, to our knowledge, new mechanistic insights into chrysanthemum waterlogging tolerance and provide a rational foundation for future research on the genetic improvement of horticultural crops for waterlogging stress tolerance.

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“…R. solanacearum belongs to PO41 strain (seed type II, race 3 biotype 2) ( He et al, 1983 ), which was cultured in a CPG medium, and then the solution was diluted to 10 8 cfu/ml (Optical Density, OD600 = 0.2). Potatoes were inoculated by the root injury irrigation method when they grew to the stage of two-week-old potato plants with 7–8 leaves ( Yu et al, 2021b ). The control group was inoculated with the same amount of water.…”

Section: Methodsmentioning

confidence: 99%

“…After the potato seedlings were inoculated with R. solanacearum or water for 72 hpi, the leaves were collected for observing the infection of R. solanacearum . The leaves were left in trypan blue solution for 24 h ( Yu et al, 2021b ). Levels of H 2 O 2 were detected using the DAB staining method by the DAB Substrate Kit (Solarbio, Beijing, China).…”

Section: Methodsmentioning

confidence: 99%

A Genome-Wide Analysis of StTGA Genes Reveals the Critical Role in Enhanced Bacterial Wilt Tolerance in Potato During Ralstonia solanacearum Infection

Tian

Suo

et al. 2022

Front. Genet.

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TGA is one of the members of TGACG sequence-specific binding protein family, which plays a crucial role in the regulated course of hormone synthesis as a stress-responsive transcription factor (TF). Little is known, however, about its implication in response to bacterial wilt disease in potato (Solanum tuberosum) caused by Ralstonia solanacearum. Here, we performed an in silico identification and analysis of the members of the TGA family based on the whole genome data of potato. In total, 42 StTGAs were predicted to be distributed on four chromosomes in potato genome. Phylogenetic analysis showed that the proteins of StTGAs could be divided into six sub-families. We found that many of these genes have more than one exon according to the conserved motif and gene structure analysis. The heat map inferred that StTGAs are generally expressed in different tissues which are at different stages of development. Genomic collinear analysis showed that there are homologous relationships among potato, tomato, pepper, Arabidopsis, and tobacco TGA genes. Cis-element in silico analysis predicted that there may be many cis-acting elements related to abiotic and biotic stress upstream of StTGA promoter including plant hormone response elements. A representative member StTGA39 was selected to investigate the potential function of the StTGA genes for further analysis. Quantitative real-time polymerase chain reaction (qRT-PCR) assays indicated that the expression of the StTGAs was significantly induced by R. solanacearum infection and upregulated by exogenous salicylic acid (SA), abscisic acid (ABA), gibberellin 3 (GA3), and methyl jasmonate (MeJA). The results of yeast one-hybrid (Y1H) assay showed that StTGA39 regulates S. tuberosum BRI1-associated receptor kinase 1 (StBAK1) expression. Thus, our study provides a theoretical basis for further research of the molecular mechanism of the StTGA gene of potato tolerance to bacterial wilt.

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StMBF1c positively regulates disease resistance to Ralstonia solanacearum via it’s primary and secondary upregulation combining expression of StTPS5 and resistance marker genes in potato

Cited by 8 publications

References 65 publications

Genome-wide characterization of the MBF1 gene family and its expression pattern in different tissues and stresses in Zanthoxylum armatum

Genome-wide characterization of the MBF1 gene family and its expression pattern in different tissues and stresses in Zanthoxylum armatum

The transcriptional coactivator CmMBF1c is required for waterlogging tolerance in Chrysanthemum morifolium

A Genome-Wide Analysis of StTGA Genes Reveals the Critical Role in Enhanced Bacterial Wilt Tolerance in Potato During Ralstonia solanacearum Infection

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