Stonewall and Brickwall: Two Partially Redundant Determinants Required for the Maintenance of Female Germline in<i>Drosophila</i>

Shukla, Vallari; Dhiman, Neena; Nayak, Prajna; Dahanukar, Neelesh; Deshpande, Girish; Ratnaparkhi, Girish S.

doi:10.1534/g3.118.200192

Cited by 6 publications

(5 citation statements)

References 73 publications

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“…It seems likely that other transcription factors of the MADF-BESS family functionally substitute for the absence of the CP60 protein. Such redundancy was previously described for MADF-BESS hinge1, hinge2, and hinge3 proteins involved in the Wg signaling pathway [44] and for Stonewall (Stwl) and Brickwall (Brwl) proteins, which are required for maintaining the female germline [77]. Also, Stwl colocalizes with binding sites for BEAF-32, ZIPIC, Pita, and Su(Hw) proteins, which recruit CP190 to chromatin in S2 cells [78] and with CP190 on larvae polytene chromosomes [79].…”

Section: Discussionmentioning

confidence: 57%

The MADF-BESS Protein CP60 Is Recruited to Insulators via CP190 and Has Redundant Functions in Drosophila

Melnikova,

Molodina,

Babosha

et al. 2023

IJMS

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Drosophila CP190 and CP60 are transcription factors that are associated with centrosomes during mitosis. CP190 is an essential transcription factor and preferentially binds to housekeeping gene promoters and insulators through interactions with architectural proteins, including Su(Hw) and dCTCF. CP60 belongs to a family of transcription factors that contain the N-terminal MADF domain and the C-terminal BESS domain, which is characterized by the ability to homodimerize. In this study, we show that the conserved CP60 region adjacent to MADF is responsible for interacting with CP190. In contrast to the well-characterized MADF-BESS transcriptional activator Adf-1, CP60 is recruited to most chromatin sites through its interaction with CP190, and the MADF domain is likely involved in protein–protein interactions but not in DNA binding. The deletion of the Map60 gene showed that CP60 is not an essential protein, despite the strong and ubiquitous expression of CP60 at all stages of Drosophila development. Although CP60 is a stable component of the Su(Hw) insulator complex, the inactivation of CP60 does not affect the enhancer-blocking activity of the Su(Hw)-dependent gypsy insulator. Overall, our results indicate that CP60 has an important but redundant function in transcriptional regulation as a partner of the CP190 protein.

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Section: Discussionmentioning

confidence: 57%

The MADF-BESS Protein CP60 Is Recruited to Insulators via CP190 and Has Redundant Functions in Drosophila

Melnikova,

Molodina,

Babosha

et al. 2023

IJMS

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“…Nevertheless, several of the DEGs are implicated in processes associated with germline maintenance and DNA repair. Among the up-regulated genes, 3 ( CALMAC_LOCUS18783 , CALMAC_LOCUS9511 , and CALMAC_LOCUS2860 ) code for proteins containing an MADF domain [ 72 ], which can also be found in the putative transcription factor s tonewall in Drosophila melanogaster , which is involved in female germline stem cell maintenance [ 73 ]. Also up-regulated is the gene CALMAC_LOCUS8201 , whose protein product contains a ULP protease domain [ 72 ]; in yeast, Ulp1 is involved in the sumoylation dynamics that play a critical role in the DNA damage response, specifically in the repair of double-strand breaks [ 74 ].…”

Section: Resultsmentioning

confidence: 99%

Increased male investment in sperm competition results in reduced maintenance of gametes

2023

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Male animals often show higher mutation rates than their female conspecifics. A hypothesis for this male bias is that competition over fertilization of female gametes leads to increased male investment into reproduction at the expense of maintenance and repair, resulting in a trade-off between male success in sperm competition and offspring quality. Here, we provide evidence for this hypothesis by harnessing the power of experimental evolution to study effects of sexual selection on the male germline in the seed beetle Callosobruchus maculatus. We first show that 50 generations of evolution under strong sexual selection, coupled with experimental removal of natural selection, resulted in males that are more successful in sperm competition. We then show that these males produce progeny of lower quality if engaging in sociosexual interactions prior to being challenged to surveil and repair experimentally induced damage in their germline and that the presence of male competitors alone can be enough to elicit this response. We identify 18 candidate genes that showed differential expression in response to the induced germline damage, with several of these previously implicated in processes associated with DNA repair and cellular maintenance. These genes also showed significant expression changes across sociosexual treatments of fathers and predicted the reduction in quality of their offspring, with expression of one gene also being strongly correlated to male sperm competition success. Sex differences in expression of the same 18 genes indicate a substantially higher female investment in germline maintenance. While more work is needed to detail the exact molecular underpinnings of our results, our findings provide rare experimental evidence for a trade-off between male success in sperm competition and germline maintenance. This suggests that sex differences in the relative strengths of sexual and natural selection are causally linked to male mutation bias. The tenet advocated here, that the allocation decisions of an individual can affect plasticity of its germline and the resulting genetic quality of subsequent generations, has several interesting implications for mate choice processes.

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“…Jarid2 and Su(var) [3][4][5][6][7] or one of the 45 D. melanogaster MADF-BESS family members (e.g. Brwl or Hng2) 29,64 , which are known to function redundantly in other tissues.…”

Section: Discussionmentioning

confidence: 99%

A nuclear architecture screen inDrosophilaidentifies Stonewall as a link between chromatin position at the nuclear periphery and germline stem cell fate

Chavan,

Isenhart,

Nguyen

et al. 2023

Preprint

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The association of genomic loci to the nuclear periphery is proposed to facilitate cell-type specific gene repression and influence cell fate decisions. However, the interplay between gene position and expression remains incompletely understood, in part because the proteins that position genomic loci at the nuclear periphery remain unidentified. Here, we used an Oligopaint-based HiDRO screen targeting ∼1000 genes to discover novel regulators of nuclear architecture inDrosophilacells. We identified the heterochromatin-associated protein, Stonewall (Stwl), as a factor promoting perinuclear chromatin positioning. In female germline stem cells (GSCs), Stwl binds and positions chromatin loci, including GSC differentiation genes, at the nuclear periphery. Strikingly, Stwl-dependent perinuclear positioning is associated with transcriptional repression, highlighting a likely mechanism for Stwl’s known role in GSC maintenance and ovary homeostasis. Thus, our study identifies perinuclear anchors inDrosophilaand demonstrates the importance of gene repression at the nuclear periphery for cell fate.

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Stonewall and Brickwall: Two Partially Redundant Determinants Required for the Maintenance of Female Germline inDrosophila

Cited by 6 publications

References 73 publications

The MADF-BESS Protein CP60 Is Recruited to Insulators via CP190 and Has Redundant Functions in Drosophila

The MADF-BESS Protein CP60 Is Recruited to Insulators via CP190 and Has Redundant Functions in Drosophila

Increased male investment in sperm competition results in reduced maintenance of gametes

A nuclear architecture screen inDrosophilaidentifies Stonewall as a link between chromatin position at the nuclear periphery and germline stem cell fate

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