Storage Effect on Phenols and on the Antioxidant Activity of Extracts from<i>Anemopsis californica</i>and Inhibition of Elastase Enzyme

Del‐Toro‐Sánchez, Carmen Lizette; Gutiérrez-Lomelí, Melesio; Lugo‐Cervantes, Eugenia; Zurita, Florentina; Robles-García, Miguel Ángel; Ruíz‐Cruz, Saúl; Aguilar, José Antonio; Rio, Juan Alfredo Morales-Del; Guerrero-Medina, Pedro Javier

doi:10.1155/2015/602136

Cited by 41 publications

(23 citation statements)

References 29 publications

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“…In addition, a correlation between short storage period and high antioxidant capacity was observed. Several studies have reported on the effects of storage on antioxidant activity of plant extracts . Similarly to our results, the antioxidant activity of orange juice and shadblow serviceberry ( Amelanchier canadensis ) decreased during storage.…”

Section: Discussionsupporting

confidence: 89%

“…Several studies have reported on the effects of storage on antioxidant activity of plant extracts. [39][40][41][42][43] Similarly to our results, the antioxidant activity of orange juice 43 Figure 1B) suggesting their ability to scavenge superoxide anions and storage did not affect SOD activity. Similar observations, no significant changes in the activities of antioxidant enzymes such as SOD, were reported for erythrocytes extracts after storage at −80°C for 6, 12, and 21 months.…”

Section: Efficacy Of Thylakoids On Repairing Uv Exposure Damagesupporting

confidence: 86%

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Study of antioxidant properties of thylakoids and application in UV protection and repair of UV‐induced damage

St-Pierre

Blondeau

Boivin

et al. 2019

J of Cosmetic Dermatology

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Background Skin is affected by environmental stress such as ultraviolet exposure. Topically applied antioxidants confer protection against this stress. Spinach thylakoid extracts are plant samples known as photosynthetic membranes containing antioxidant molecules able to dissipate excess of energy and oxidative stress. Methods Antioxidant contents and activities were tested in thylakoid extracts stored for different periods at 4°C to compare their efficacities. Cytotoxicity of thylakoids was tested on human THP‐1 cells along with the capacity to protect from oxidative stress using flow cytometry. Protection of thylakoids against ultraviolet was tested on engineered human skin using two formulations and evaluated by electronic microscopy. Results Results indicate that thylakoid extracts possess antioxidant molecules that were not significantly affected by storage at 4°C whereas photosynthetic activity was storage‐dependent. Thylakoid extracts were not cytotoxic to human THP‐1 cells, and three extracts protected cells against reactive oxygen species. Moreover, formulation comprising 0.1% or 0.01% of thylakoids and sunscreen provided a synergetic protection against UV exposure. Thylakoid extracts mixed with a neutral cream were also able to repair UV damages on engineered human skin. Conclusions Thylakoid extracts contained various antioxidant molecules, and their properties were maintained in over storage at 4°C for more than 72 months. Molecules and enzymes present in thylakoid extracts are involved in protecting and restoring the harmful effects of UV exposure. The involvement of antioxidant molecules such as carotenoids, SOD, and Fe‐S clusters in cellular and regulatory metabolic reactions may explain the observed protective effects.

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Section: Discussionsupporting

confidence: 89%

Section: Efficacy Of Thylakoids On Repairing Uv Exposure Damagesupporting

confidence: 86%

Study of antioxidant properties of thylakoids and application in UV protection and repair of UV‐induced damage

St-Pierre

Blondeau

Boivin

et al. 2019

J of Cosmetic Dermatology

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“…Del-Toro-Sánchez et al reported a massive 36% reduction in the TPC of the herb, Anemopsis californica , extracts stored at 50, 25 and 4 °C with exposure to light after 180 days. The authors reported that high temperature and light degraded the structure of the polyphenols [ 22 ]. Qu et al investigated the storage stability of phenolic compounds in sterilised liquid pomegranate peel extracts at 4 °C under light and dark conditions.…”

Section: Resultsmentioning

confidence: 99%

Impact of Storage Conditions on the Stability of Predominant Phenolic Constituents and Antioxidant Activity of Dried Piper betle Extracts

et al. 2018

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The phenolic constituents in Piper betle are well known for their antioxidant potential; however, current literature has very little information on their stability under the influence of storage factors. Present study evaluated the stability of total phenolic content (TPC) and antioxidant activity together with individual phenolic constituents (hydroxychavicol, eugenol, isoeugenol and allylpyrocatechol 3,4-diacetate) present in dried Piper betle’s extract under different storage temperature of 5 and 25 °C with and without light for a period of six months. Both light and temperature significantly influenced TPC and its corresponding antioxidant activity over time. More than 95% TPC and antioxidant activity was retained at 5 °C in dark condition after 180 days of storage. Hydroxychavicol demonstrated the best stability with no degradation while eugenol and isoeugenol displayed moderate stability in low temperature (5 °C) and dark conditions. 4-allyl-1,2-diacetoxybenzene was the only compound that underwent complete degradation. A new compound, 2,4-di-tert-butylphenol, was detected after five weeks of storage only in the extracts exposed to light. Both zero-order and first-order kinetic models were adopted to describe the degradation kinetics of the extract’s antioxidant activity. Zero-order displayed better fit with higher correlation coefficients (R2 = 0.9046) and the half-life was determined as 62 days for the optimised storage conditions (5 °C in dark conditions).

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“…The degradation of the compounds following extract storage that was observed in this study was consistent with previous findings [ 53 ], where the total phenolic content in Cornelian cherries extracts was found to decrease after being stored at 2 °C for up to 60 days. A drastic decrease was observed in the total phenolic and flavonoid content of Anemopsis californica extracts after being stored at 4 °C [ 54 ]. The decrease in total phenolics and flavonoids after prolonged storage was caused by various factors including oxidation by enzymes such as glycosidase, phenolase, and polyphenol oxidase (PPO), degradation of compounds, the polymerization of these compounds with proteins, and the conversion between free and bound phenolic substances [ 55 , 56 , 57 ].…”

Section: Resultsmentioning

confidence: 99%

Vermicompost Supplementation Improves the Stability of Bioactive Anthocyanin and Phenolic Compounds in Clinacanthus nutans Lindau

et al. 2018

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This project studied the effect of vermicompost application on the composition of bioactive anthocyanin and phenolic compounds, and the antioxidant activity of Clinacanthus nutans. The correlation between the bioactive constituents and antioxidant capacity was also evaluated. In this project, a field study was conducted using a randomized complete block design (RCBD) with four treatment groups, including control plants (CC), plants supplied with chemical fertilizer (CF), plants supplied with vermicompost (VC), and plants supplied with mixed fertilizer (MF). The leaves of C. nutans from all treatment groups were harvested, subjected to solvent extraction, and used for quantification of total anthocyanin content (TAC), total phenolic content (TPC), and total flavonoid content (TFC). The initial antioxidant activity of the extracts was evaluated using 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays, as well as after two and four weeks of storage at −20 °C and 4 °C. Data analysis showed that CC plants contained the highest TAC (2180.14 ± 338.43 µg/g dry weight) and TFC (276.25 ± 3.09 mg QE/g dry weight). On the other hand, CF plants showed the highest TPC (181.53 ± 35.58 mg GAE/g dry weight). Moreover, we found that CC plants had the highest antioxidant potential against DPPH radicals whereas MF plants showed the lowest antioxidant potential. After four weeks of extract storage at −20 °C and 4 °C, the TPC, TFC, TAC, and antioxidant potential of the extracts decreased. Extracts from VC showed the lowest percentage of total phenolic and total flavonoid loss after extract storage at −20 °C and 4 °C compared with other plant extracts. At this juncture, it could be deduced that the application of vermicompost had little effect on the expression of phenolics, flavonoids, or anthocyanin in C. nutans. However, the extract from plants treated with vermicompost (VC and MF) showed better stability compared with CC and CF after extract storage at different temperatures.

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Storage Effect on Phenols and on the Antioxidant Activity of Extracts fromAnemopsis californicaand Inhibition of Elastase Enzyme

Cited by 41 publications

References 29 publications

Study of antioxidant properties of thylakoids and application in UV protection and repair of UV‐induced damage

Study of antioxidant properties of thylakoids and application in UV protection and repair of UV‐induced damage

Impact of Storage Conditions on the Stability of Predominant Phenolic Constituents and Antioxidant Activity of Dried Piper betle Extracts

Vermicompost Supplementation Improves the Stability of Bioactive Anthocyanin and Phenolic Compounds in Clinacanthus nutans Lindau

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