STR polymorphisms in Sri Lanka: evaluation of forensic utility in identification of individuals and parentage testing

Manamperi, A.; Hapuarachchi, Chanditha; Gunawardene, Nilmini Silva; Bandara, Anura; Dayanath, Damsiri; Abeyewickreme, W.

doi:10.4038/cmj.v54i3.1201

Cited by 8 publications

(5 citation statements)

References 12 publications

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“…Genomic DNA was extracted from oral mucosa exfoliative cell samples with the Chelex-100 method (Manamperi et al 2009). The genetic variations were genotyped with polymerase chain reaction (PCR)-single-strand conformation polymorphism (SSCP), PCR-restriction fragment length polymorphism (RFLP), or amplified fragment length polymorphism methods.…”

Section: Genetic Variations Selecting and Genotypingmentioning

confidence: 99%

Association Analysis Between 12 Genetic Variants of Ten Genes and Personality Traits in a Young Chinese Han Population

Gong

Zheng²,

Zhang

et al. 2010

J Mol Neurosci

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Some genes involved in neurotransmission synthesis and transmission have been hypothesized to affect personality traits. To investigate the possible roles of these genes in personality traits of 16 Personality Factor Questionnaire, we performed a population-based study in a young Chinese Han cohort. In the study, we selected some functional variations in ten candidate genes (COMT, DBH, DRD(2), DRD(3), DAT, MAOA, GRM(1), GRIN2B, 5-TH(2A), and 5-TH(6)) encoding components in dopamine, glutamate, and 5-hydroxytryptamine pathways. The results showed the T102C in 5-TH(2A) was associated with X3 (emotional and quiet alertness) and B (reasoning) (F = 4.71 and 6.23; p = 0.009 and 0.002), Val158Met in COMT with E (dominance) (F = 7.01; p = 0.0009), while the variations in DBH, DRD(2), DRD(3), MAOA, GRM(1), GRIN2B, and 5-TH(6) were not associated with any of the personality traits. This finding suggests that T102C in 5-TH(2A) and Val158Met in COMT play roles in some human personality traits.

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Section: Genetic Variations Selecting and Genotypingmentioning

confidence: 99%

Association Analysis Between 12 Genetic Variants of Ten Genes and Personality Traits in a Young Chinese Han Population

Gong

Zheng²,

Zhang

et al. 2010

J Mol Neurosci

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“…Other than the applications related to infectious and inherited diseases, DNA fingerprinting to resolve disputed paternity cases and forensic cases is the other strand of molecular based investigations widely being used in Sri Lanka (at present offered only by the Molecular Medicine Unit of the Faculty of Medicine, University of Kelaniya and Genetech Diagnostics, Colombo; [11]). This new technology has had a wide ranging impact on both the civil and criminal branches of the judiciary, and provision of these services has helped to resolve in 1-2 months paternity disputes which have dragged on in some cases for more than 9-10 years, and importantly, to exonerate innocent individuals from criminal charges.…”

Section: Development Of New Diagnostics and Their Applicationsmentioning

confidence: 99%

Capacity Building in Genomics Medicine and Molecular Diagnostics: The Case of Sri Lanka

Manamperi

Huzair²

2012

CPPM

Self Cite

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In this paper, we advance the extant health technology innovation frames on global personalized medicine by highlighting the need to rethink genomics medicine in real-life settings -including situations where populations are frequently faced with natural disasters or man-made conflicts. We identify the steps towards building sufficient capacity to effectively harness and integrate genomic medicine and molecular diagnostics in order to benefit global society including those in resource-limited settings and post-war capacity building contexts. Surprisingly, despite a great number of populations currently living in developing countries, including in a state of post-war or conflict resolution context, the public health pharmacogenomics community has largely neglected this crucial dimension in biomedical literature. By exploring the particular case of Sri-Lanka in this paper, we are able to investigate the obstacles commonly faced by low and middle income countries similarly afflicted by crises, natural disasters, conflict and the need for improved, more cost effective health care. Sri Lanka has a relatively strong platform for launching molecular diagnostic technology, including a well networked set of primary, secondary and tertiary care institutions, a small but burgeoning private health and research sector and a strong science base in its universities. Despite this, there has been slow uptake and exploitation of novel molecular diagnostics due to various factors such as a weak regulatory framework and high costs associated with the import of molecular reagents and import and maintenance of equipment. In summary, as a way forward for health technology assessment in resource-limited countries, this paper brings to the fore an integrated discourse on real-life experiences and putative solutions on genomics and molecular diagnostic medicine. For a comprehensive discussion in the nascent field of public health pharmacogenomics, post-war and post-conflict capacity building on biotechnologies such as genomics is essential.

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“…5'-CCTAGTGGATGATAAGAATAATCAGTATG-3' and R: 5'-GGACAGATGATAAATACATAGGATGGAT GG-3', FGA with arrangement F: 5'-GCCCCATAGGTTTTGAACTCA-3' and R: 5'-TGATTTGTCTGTAATTGCCAGC-3', TH01 with arrangement F: 5'-CTGGGCACGTGAGGG CAGCGTCT-3' and R: 5'-TGCCGGAAGTCCATCCTCA CAGTC-3' (18)(19)(20). The PCR results were visualized using 2% acrylamide gel and then stained using silver staining (21)(22)(23). The primary targets were 123-171 bp for vWA, 322-444 bp for FGA, and 156-195 bp for TH01.…”

Section: This Study Has Been Deemed Ethically By the Healthmentioning

confidence: 99%

Amplification of vWA, FGA, and TH01 loci of DNA Samples Isolated From Ring Stored at Room Temperature

Furqoni,

Ayu Megasari,

Yudianto

et al. 2023

MJMHS

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Introduction: Acts of criminal behaviour that occur have various modes and motives. Also, criminals always try to hide or eliminate evidence at the crime scene. In most cases, police or forensic experts often find DNA on items at the crime scene. One of these items is a ring, which is an item that humans often wear. Methods: This study used 24 samples of rings that had been worn for 8 hours and were incubated at room temperature. All these 24 samples then were distinguished into 4 groups, in which each group was consisting of 6 samples and incubated for 0, 1, 3, and 7-days. DNA identification was then carried out using UV spectrometer for DNA quantification and DNAzol method for DNA extraction. Results: The mean result of DNA quantification on day 0 (control) was 1020,833 ± 0.28903 ng/μL, day 1 was 546 ± 0.093569 ng/μL, day 3 was 1066.333 ± 0.117372 ng/μL, and day 7 was 1054.083 ± 0.070733 ng/μL. PCR process used STR primers with loci vWA, FGA, and TH01 and visualization used the silver nitrate method. Conclusion: The final results showed that all samples could be amplified using 3 STR loci, namely vWA, FGA, and TH01.

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STR polymorphisms in Sri Lanka: evaluation of forensic utility in identification of individuals and parentage testing

Cited by 8 publications

References 12 publications

Association Analysis Between 12 Genetic Variants of Ten Genes and Personality Traits in a Young Chinese Han Population

Association Analysis Between 12 Genetic Variants of Ten Genes and Personality Traits in a Young Chinese Han Population

Capacity Building in Genomics Medicine and Molecular Diagnostics: The Case of Sri Lanka

Amplification of vWA, FGA, and TH01 loci of DNA Samples Isolated From Ring Stored at Room Temperature

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