Strain differences in the mycelium of the ectomycorrhizal Tuber borchii

Saltarelli, Roberta; Ceccaroli, Paola; Cesari, Paola; Zeppa, Sabrina Donati; Potenza, Lucia; Stocchi, Vilberto

doi:10.1017/s0953756299008898

“…It would appear that different morphological characteristics of the mycorrhizas may also be determined by intra‐specific variability in the symbiont fungus. This has already been shown to occur in T. borchii [7,22].…”

Section: Discussionmentioning

confidence: 52%

Comparative study and molecular characterization of ectomycorrhizas inTilia americanaandQuercus pubescenswithTuber brumale

Giomaro

¹

,

Sisti

²

,

Zambonelli

³

et al. 2002

FEMS Microbiology Letters

22

0

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Mycorrhizas of Tuber brumale on Quercus pubescens and Tilia americana were obtained in vitro using micropropagated plantlets. Mycelium pure cultures were used for inoculation. Both the mycelium used for the inoculations, as well as the mycorrhizas which were obtained, were identified using several molecular approaches: analysis of the ITS region, polymerase chain reaction (PCR) specific primers and sequencing. The mycorrhizas were described from a morphological standpoint. Some of their biometric characteristics were different in bass-wood than they were in oak, thus showing the influence of the host plant on several of the morphological features believed to be necessary for the identification of the species. Considering the variability of their morphological characteristics, molecular analysis proved to be a necessary tool for the recognition of the mycorrhizas of Tuber spp.

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“…It would appear that di¡erent morphological characteristics of the mycorrhizas may also be determined by intra-speci¢c variability in the symbiont fungus. This has already been shown to occur in T. borchii [7,22].…”

Section: Discussionmentioning

confidence: 53%

Comparative study and molecular characterization of ectomycorrhizas in Tilia americana and Quercus pubescens with Tuber brumale

Giomaro

¹

2002

FEMS Microbiology Letters

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Mycorrhizas of Tuber brumale on Quercus pubescens and Tilia americana were obtained in vitro using micropropagated plantlets. Mycelium pure cultures were used for inoculation. Both the mycelium used for the inoculations, as well as the mycorrhizas which were obtained, were identified using several molecular approaches: analysis of the ITS region, polymerase chain reaction (PCR) specific primers and sequencing. The mycorrhizas were described from a morphological standpoint. Some of their biometric characteristics were different in bass-wood than they were in oak, thus showing the influence of the host plant on several of the morphological features believed to be necessary for the identification of the species. Considering the variability of their morphological characteristics, molecular analysis proved to be a necessary tool for the recognition of the mycorrhizas of Tuber spp.

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“…Several studies on the C utilization by several Tuber species have been reported (Bonfante & Fontana, 1973;Mamoun & Olivier, 1991;Saltarelli et al, 1998Saltarelli et al, , 1999Ceccaroli, Saltarelli, Cesari, Zambonelli, & Stocchi, 2001;Bedade et al, 2018). For instance, T. melanosporum utilizes various C sources, such as monosaccharide (arabinose, fructose, galactose, glucose, and mannose), disaccharide (lactose, maltose, and sucrose), polysaccharide (dextrin and starch), and sugar alcohol (mannitol) (Bonfante & Fontana, 1973;Mamoun & Olivier, 1991).…”

mentioning

confidence: 99%

“…For instance, T. melanosporum utilizes various C sources, such as monosaccharide (arabinose, fructose, galactose, glucose, and mannose), disaccharide (lactose, maltose, and sucrose), polysaccharide (dextrin and starch), and sugar alcohol (mannitol) (Bonfante & Fontana, 1973;Mamoun & Olivier, 1991). In addition, the C utilization ability of T. borchii varies among different strains (Saltarelli et al, 1999;Ceccaroli et al, 2001). This is true for T. japonicum.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Physiological characteristics of pure cultures of a white-colored truffle <i>Tuber japonicum</i>

Nakano

¹

,

Kinoshita

²

,

Obase

³

et al. 2022

Mycoscience

2

0

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Truffles, primarily belonging to the genus Tuber, are ascomycetous ectomycorrhizal (EM) fungi that form symbiotic associations with diverse tree species (Pacioni & Comandini, 1999;Gryndler, 2016). Several Tuber species, such as T. magnatum Picco (Italian white truffle), T. melanosporum Vittad. (Périgord black truffle), T. aestivum Vittad. (summer truffle), and T. borchii Vittad. (Bianchetto truffle), produce edible fruiting bodies with unique aromas (Hall & Haslam, 2012), and they are globally traded at high prices (Hall, Brown, & Zambonelli, 2007). Various trials have been conducted to develop the proper management of truffle production in fields (Delmas, 1978;Pacioni & Comandini, 1999;Hall et al., 2007). To date, artificial cultivation of several truffle species is accomplished using EM plants inoculated with ascospores or pure-cultured mycelia of these species (Iotti,

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Strain differences in the mycelium of the ectomycorrhizal Tuber borchii

Cited by 7 publications

References 27 publications

Comparative study and molecular characterization of ectomycorrhizas inTilia americanaandQuercus pubescenswithTuber brumale

Comparative study and molecular characterization of ectomycorrhizas inTilia americanaandQuercus pubescenswithTuber brumale

Comparative study and molecular characterization of ectomycorrhizas in Tilia americana and Quercus pubescens with Tuber brumale

Physiological characteristics of pure cultures of a white-colored truffle <i>Tuber japonicum</i>

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