Strain improvement in industrial microorganisms by recombinant DNA techniques

Recombinant plasmids

“…Host vector systems have been developed for many other Gram-positive bacteria such as the corynebacteria, because of their biosynthetic potential, particularly for amino acid production (Santamaria, et al, 1987;Trautwetter et al, 1987;Wolf et al, 1989: Patek et al, 1989. A comprehensive list of host vector systems for Gram-positive bacteria of industrial interest has been compiled by Schwab (1988).…”

“…The use of fungal plasmids as a basis for vector development has been successful in only a few species, including Neurospora crassa (Stohl and Labowitz, 1983) and Podospora anserina (Stahl et al, 1982). Schwab (1988) summarizes the properties of cloning systems for the filamentous fungi. Schwab (1988) summarizes the properties of cloning systems for the filamentous fungi.…”

“…The subject has been discussed imaginatively in a number of reviews, including Imanaka (1986), OECD (1986), ECRAB (1986), Hutter (1987), Driesel (1988, Schwab (1988), and Malik (1989). The subject has been discussed imaginatively in a number of reviews, including Imanaka (1986), OECD (1986), ECRAB (1986), Hutter (1987), Driesel (1988, Schwab (1988), and Malik (1989).…”

“…Schwab (1988) discusses the potential for altering traits which affect the productivity and cost-effectiveness of industrial fermentations, including specific production rate, yield, product quality, product recovery and substrate utilization. DNA manipulation technology affects manufacturing industry in two major ways, particularly in the food and beverage, pharmaceutical and health-care sectors.…”

“…Many vectors which are derived from pBR322 are segregationally unstable because control sequences are absent (Primrose et al, 1984) and stability can be increased by cloning partition functions from other plasmids (for example, Yukawa et al, 1988;Schwab, 1988). Many vectors which are derived from pBR322 are segregationally unstable because control sequences are absent (Primrose et al, 1984) and stability can be increased by cloning partition functions from other plasmids (for example, Yukawa et al, 1988;Schwab, 1988).…”