Strain-specific interspecies interactions between co-isolated pairs of Staphylococcus aureus and Pseudomonas aeruginosa from patients with tracheobronchitis or bronchial colonization

Gomes-Fernandes, Meissiner; Gómez, Andromeda-Celeste; Bravo, Marc; Huedo, Pol; Coves, Xavier; Prat‐Aymerich, Cristina; Gibert, Isidre; Lacoma, Alícia; Yero, Daniel

doi:10.1038/s41598-022-07018-5

Cited by 16 publications

(9 citation statements)

References 71 publications

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“…In terms of inhibitory effects against gram-positive bacteria, P. aeruginosa strain ATCC27853 showed notable activity, exhibiting strong inhibition against S. aureus ATCC29213 and B. cereus ATCC14579 after 48 h of incubation. However, it showed no inhibition against S. aureus ATCC29213 and B. cereus ATCC14579 after 24 h. Similar studies have reported the varying inhibitory effects of P. aeruginosa strains against different gram-positive bacteria, highlighting the strain-specific nature of these effects [33][34][35].…”

Section: Discussionsupporting

confidence: 63%

Enzyme Production and Inhibitory Potential of Pseudomonas aeruginosa: Contrasting Clinical and Environmental Isolates

Aqel,

Sannan,

Foudah

et al. 2023

Antibiotics

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(1) Background: This study summarizes the findings of two studies investigating the inhibitory effects of Pseudomonas aeruginosa strains from clinical and environmental sources against gram-positive and gram-negative bacteria and fungi. The studies also analyzed the correlation between enzyme production and inhibitory effects to gain insights into the antimicrobial capabilities of P. aeruginosa strains; (2) Methods: Both studies employed similar methodologies, including the use of disk diffusion and well diffusion methods to assess the inhibitory effects of P. aeruginosa strains against target pathogens. Enzyme production was analyzed through various biochemical assays to determine the diversity and frequencies of enzyme secretion among the strains; (3) Results: A comparative analysis of enzyme production in P. aeruginosa strains from clinical sources revealed significant variations in enzyme production, with hemolysin and protease being the most commonly produced enzymes. Gelatinase production showed lower rates, whereas chondroitinase and hyaluronidase were absent or occurred less frequently. In contrast, a comparative analysis of enzyme production in environmental isolates showed different patterns, indicating adaptation to environmental conditions. Pyocyanin production was absent in all environmental isolates. The inhibitory effects against gram-positive and gram-negative bacteria varied among different P. aeruginosa strains, with strain-specific variations observed. Limited inhibitory effects were observed against fungi, primarily toward gram-positive bacteria; (4) Conclusions: The findings highlight the strain-specific nature of inhibitory effects and enzyme production in P. aeruginosa strains. The correlation between enzyme production and inhibitory effects against gram-positive bacteria suggest a potential role of specific enzymes, such as hemolysin and protease, in the antimicrobial activity. The complexity of the relationship between enzyme production and the inhibition of different pathogens requires further investigation. The results emphasize the potential of P. aeruginosa strains as sources for antimicrobial strategies, particularly against gram-positive bacteria. Future research should focus on understanding the mechanisms underlying these inhibitory effects and exploring their therapeutic applications.

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Section: Discussionsupporting

confidence: 63%

Enzyme Production and Inhibitory Potential of Pseudomonas aeruginosa: Contrasting Clinical and Environmental Isolates

Aqel,

Sannan,

Foudah

et al. 2023

Antibiotics

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“…The strain-specific nature of synergy in mixed-species biofilms on the one hand emphasizes the significance of strains, rather than species, in studying bacterial interactions and on the one hand, it has implications for the development of synthetic bacterial communities. The role of inter-strain phenotypic diversity in modulating interactions in mixed species biofilms has been reported, mainly in dual-species biofilm settings ( Gomes-Fernandes et al, 2022 ). L. monocytogenes strains of food origin have been reported to show large variations in competitive growth in different mixed-species biofilm settings ( Heir et al, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

Synergistic interactions in multispecies biofilm combinations of bacterial isolates recovered from diverse food processing industries

Sadiq¹,

Reu²,

Burmølle

et al. 2023

Front. Microbiol.

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Most biofilms within the food industry are formed by multiple bacterial species which co-exist on surfaces as a result of interspecies interactions. These ecological interactions often make these communities tolerant against antimicrobials. Our previous work led to the identification of a large number (327) of highly diverse bacterial species on food contact surfaces of the dairy, meat, and egg industries after routine cleaning and disinfection (C&D) regimes. In the current study, biofilm-forming ability of 92 bacterial strains belonging to 26 genera and 42 species was assessed and synergistic interactions in biofilm formation were investigated by coculturing species in all possible four-species combinations. Out of the total 455 four-species biofilm combinations, greater biofilm mass production, compared to the sum of biofilm masses of individual species in monoculture, was observed in 34 combinations. Around half of the combinations showed synergy in biofilm mass > 1.5-fold and most of the combinations belonged to dairy strains. The highest synergy (3.13-fold) was shown by a combination of dairy strains comprising Stenotrophomonas rhizophila, Bacillus licheniformis, Microbacterium lacticum, and Calidifontibacter indicus. The observed synergy in mixed biofilms turned out to be strain-specific rather than species-dependent. All biofilm combinations showing remarkable synergy appeared to have certain common species in all combinations which shows there are keystone industry-specific bacterial species which stimulate synergy or antagonism and this may have implication for biofilm control in the concerned food industries.

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“…Staphylococcus aureus) during interspecies competition and establishment of the pathogen in its niche [49]. A co-isolated pair of S. aureus and P. aeruginosa from patients with tracheobronchitis or bronchial colonization revealed that P. aeruginosa exoproducts impacted biofilm formation and decreased in vitro growth of its S. aureus counterpart, while S. aureus did not impair biofilm formation and triggered swarming motility in P. aeruginosa [50].…”

Section: Identification Of New Potential Therapeutic Targets Against ...mentioning

confidence: 99%

Recent advances in therapeutic targets identification and development of treatment strategies towards Pseudomonas aeruginosa infections

et al. 2023

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The opportunistic human pathogen Pseudomonas aeruginosa is the causal agent of a wide variety of infections. This non-fermentative Gram-negative bacillus can colonize zones where the skin barrier is weakened, such as wounds or burns. It also causes infections of the urinary tract, respiratory system or bloodstream. P. aeruginosa infections are common in hospitalized patients for which multidrug-resistant, respectively extensively drug-resistant isolates can be a strong contributor to a high rate of in-hospital mortality. Moreover, chronic respiratory system infections of cystic fibrosis patients are especially concerning, since very tedious to treat. P. aeruginosa exploits diverse cell-associated and secreted virulence factors, which play essential roles in its pathogenesis. Those factors encompass carbohydrate-binding proteins, quorum sensing that monitor the production of extracellular products, genes conferring extensive drug resistance, and a secretion system to deliver effectors to kill competitors or subvert host essential functions. In this article, we highlight recent advances in the understanding of P. aeruginosa pathogenicity and virulence as well as efforts for the identification of new drug targets and the development of new therapeutic strategies against P. aeruginosa infections. These recent advances provide innovative and promising strategies to circumvent infection caused by this important human pathogen.

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Strain-specific interspecies interactions between co-isolated pairs of Staphylococcus aureus and Pseudomonas aeruginosa from patients with tracheobronchitis or bronchial colonization

Cited by 16 publications

References 71 publications

Enzyme Production and Inhibitory Potential of Pseudomonas aeruginosa: Contrasting Clinical and Environmental Isolates

Enzyme Production and Inhibitory Potential of Pseudomonas aeruginosa: Contrasting Clinical and Environmental Isolates

Synergistic interactions in multispecies biofilm combinations of bacterial isolates recovered from diverse food processing industries

Recent advances in therapeutic targets identification and development of treatment strategies towards Pseudomonas aeruginosa infections

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