Strain-specific microsatellite markers in the entomopathogenic fungus Beauveria brongniartii

Enkerli, J.; Widmer, Franco; Gessler, C.; Keller, Siegfried

doi:10.1016/s0953-7562(08)61970-x

Cited by 72 publications

(71 citation statements)

References 23 publications

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“…The characterization of B. bassiana was based on the description and measurement of structures of agronomic interest [6], on its physiological [7] and pathogenic characteristics, [8] and on the results of isozymatic studies [9]. The characterization of B. bassiana was carried out using different kinds of DNA markers [10][11][12], RFLPs and RAPDs [13,14], ITSs [15], microsatellites (SSRs) and minisatellites [15][16][17]. The molecular variation found between Beauveria isolates is related to the insect host range, and was assessed using RFLP, ITSs [18,19] and isozymes [20,21].…”

Section: Introductionmentioning

confidence: 99%

The molecular diversity of different isolates of Beauveria bassiana (Bals.) Vuill. as assessed using intermicrosatellites (ISSRs)

Estrada¹,

Camacho

Benito

2007

Cellular and Molecular Biology Letters

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Inter-microsatellite PCR (ISSR-PCR) markers were used to identify and to examine the genetic diversity of eleven Beauveria bassiana isolates with different geographic origins. The variability and the phylogenetic relationships between the eleven strains were analyzed using 172 ISSR-PCR markers. A high level of polymorphism (near 80%) was found using these molecular markers. Seven different isolates showed exclusive bands, and ISSR primer 873 was able to distinguish between all the strains. The dendrogram obtained with these markers is robust and in agreement with the geographical origins of the strains. All the isolates from the Caribbean region were grouped together in a cluster, while the other isolates grouped in the other cluster. The similarity exhibited between the two clusters was less than 50%. This value of homology shows the high genetic variability detected between the isolates from the Caribbean region and the other isolates. ISSR-PCR markers provide a quick, reliable and highly informative system for DNA fingerprinting, and allowed the identification of the different B. bassiana isolates studied.

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Section: Introductionmentioning

confidence: 99%

The molecular diversity of different isolates of Beauveria bassiana (Bals.) Vuill. as assessed using intermicrosatellites (ISSRs)

Estrada¹,

Camacho

Benito

2007

Cellular and Molecular Biology Letters

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“…The mycelium was harvested by filtration (Enkerli et al 2001) and frozen at -70°C. Frozen mycelium was lyophilized and homogenized with a FastPrep FP120 homogenizer (MP Biomedicals, Santa Ana, CA, USA) using 0.15 g of glass beads (1 mm diameter) at a speed of 6 m s -1 for 10 s. Genomic DNA was extracted using the NucleoSpin Plant II DNA extraction kit according to the manufacturer's protocol (Macherey-Nagel, Germany).…”

Section: Identification Of Re-isolated Fungal Strainsmentioning

confidence: 99%

Preventive application of an entomopathogenic fungus in cover crops for wireworm control

et al. 2017

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Efficacy of the Metarhizium brunneum Petch (Hypocreales: Clavicipitaceae) strain ART2825 for control of wireworms (Agriotes obscurus (L.), Coleoptera: Elateridae) was examined in a semi-field pot experiment. Pots were treated in late summer during sowing of spring oat as a cover crop. Survival of wireworms was assessed four weeks after their release in October 2013, and 30 weeks after release in April 2014. Viability and persistence of the fungus was determined by counting colony forming units from substrate samples and microsatellite analyses of recovered Metarhizium isolates. The number of colonies detected in the substrate in October 2013 increased with increasing concentrations of applied conidia, and no significant reduction was observed at the second evaluation date in April 2014. Increasing conidia application rates significantly increased mycosis and reduced wireworm survival, to a level comparable to that of treatment using insecticide-coated oat seeds. The preventive application of M. brunneum conidia to reduce wireworm populations in cover crops, preceding a damage-sensitive crop like potatoes, may be a promising biocontrol strategy.

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“…Other well known, but less specific mycoinsecticides used in repeated control of various insects are the hyphomycetes Beauveria bassiana and Metarhizium anisopliae (for further details on fungal control of insect pests see Shah & Pell 2003). Genetic markers [microsatellites, randomly amplified polymorphic DNA (RAPD) markers, restriction fragment length polymorphism (RFLP); for more detailed explanations see Chapter 8 of this book] have been defined to monitor Beauveria and Metarhizium strains in the field (Leal et al 1994, Maurer et al 1997, Bidochka et al 2001, Enkerli et al 2001.…”

Section: Biological Control Of Harmful Insectsmentioning

confidence: 99%

Wood production, wood technology, and biotechnological impacts

Kües¹

2007

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Strain-specific microsatellite markers in the entomopathogenic fungus Beauveria brongniartii

Cited by 72 publications

References 23 publications

The molecular diversity of different isolates of Beauveria bassiana (Bals.) Vuill. as assessed using intermicrosatellites (ISSRs)

The molecular diversity of different isolates of Beauveria bassiana (Bals.) Vuill. as assessed using intermicrosatellites (ISSRs)

Preventive application of an entomopathogenic fungus in cover crops for wireworm control

Wood production, wood technology, and biotechnological impacts

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