Strand-specific RNA sequencing in pig testes identifies developmentally regulated genes and circular RNAs

Ran, Maoliang; Wang, Bo; Chen, Bin; Wu, Maisheng; He, Chao; Zhang, Shanwen

doi:10.1007/s13258-017-0576-x

Cited by 8 publications

(8 citation statements)

References 41 publications

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“…Ran et al predicted 10,000 circRNAs in pig testes at day 30‐old and day 180‐old by RNA sequencing (Ran et al., 2017). Results provide a new clue to further unravel functions of circRNAs in the regulatory reproduction mechanisms.…”

Section: Methodsmentioning

confidence: 99%

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Biological functions of circRNAs and their progress in livestock and poultry

Huang

Wang

Zhang

et al. 2020

Reprod Domestic Animals

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CircRNAs are a class of newly discovered RNAs extensively expressed in eukaryotic cells (Jeck et al., 2013). Compared with conventional linear RNAs (containing 5′ and 3′ ends), circRNAs are covalently closed RNA molecules. It was recognized as a class of functional RNA that differs from miRNA, piRNA and lncRNA due to its character of circularity (Xu, Wu, Han, Zhao, & Song, 2017). CircRNAs were first found in RNA viruses as a viroid as early as in 1976 (Sanger, Klotz, Riesner, Gross, & Kleinschmidt, 1976). And then in 1986, it was also founded that the HDV, an animal virus, has a circRNA genome, which were considered to be a characteristic of viroids (Kos, Dijkema, Arnberg, van der Meide, & Schellekens, 1986). Due to limitation of technology at that time, circRNAs were less reported and were once considered as by-products of mis-splicing or regarded as a meaningless abnormally cut RNA, which never got any attention by some researchers because traditional molecular techniques employed in RNA analysis were not able to detect circRNAs because they did not have cap of 5′ end and tail of 3′ end (Jeck & Sharpless, 2014). It was not until 1991 that endogenous circRNAs were demonstrated in human cells, which originated from exons (Nigro et al., 1991). Since then, with advancement of bioinformatics analysis and RNA sequencing technologies, circRNA has attracted more and more attention of many scientists because it has been reported to be ubiquitously present in eukaryotic organisms and have longer half-lives than linear RNAs (Memczak et al., 2013;

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Section: Methodsmentioning

confidence: 99%

“…Ran et al predicted 10,000 circRNAs in pig testes at day 30old and day 180-old by RNA sequencing (Ran et al, 2017) (Huang, Shen, et al, 2018). Moreover, 366 circRNAs were detected as significantly differentially expressed in two breeds.…”

Section: Circrnas and Pigmentioning

confidence: 99%

Biological functions of circRNAs and their progress in livestock and poultry

Huang

Wang

Zhang

et al. 2020

Reprod Domestic Animals

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“…Real-time quantitative PCR (qPCR) was performed as described in our previous studies ( Ran et al, 2016 , 2017 , 2018 ; Weng et al, 2017a ). Total RNA was extracted using TRIzol reagent (Invitrogen, United States), according to the manufacturer’s protocols.…”

Section: Methodsmentioning

confidence: 99%

miR-222 Suppresses Immature Porcine Sertoli Cell Growth by Targeting the GRB10 Gene Through Inactivating the PI3K/AKT Signaling Pathway

et al. 2020

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Sertoli cells are central and essential coordinators of spermatogenesis. Accumulating evidence has demonstrated that miRNAs participate in the regulation of Sertoli cell growth. However, the functions and the regulatory mechanisms of miRNAs in Sertoli cells of domestic animals remain largely unknown. Here we report that miR-222 overexpression repressed cell cycle progression and proliferation and promoted the apoptosis of immature porcine Sertoli cells, whereas miR-222 inhibition resulted in the opposite result. miR-222 directly targeted the 3′-UTR of the GRB10 gene and inhibited its mRNA abundance. An siRNA-induced GRB10 knockdown showed similar effects as did miR-222 overexpression on cell proliferation and apoptosis and further attenuated the role of miR-222 inhibition. Furthermore, both miR-222 overexpression and GRB10 inhibition repressed the phosphorylation of PI3K and AKT, the key elements of the PI3K/AKT signaling pathway, whereas GRB10 inhibition offsets the effects of the miR-222 knockdown. Overall, we concluded that miR-222 suppresses immature porcine Sertoli cell growth by targeting the GRB10 gene through inactivation of the PI3K/AKT signaling pathway. This study provides novel insights into the epigenetic regulation of porcine spermatogenesis by determining the fate of Sertoli cells.

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“…Circular RNAs (circRNAs), a novel type of endogenous regulatory noncoding RNA, are characterized by the stable closed-loop structure and lack of 5 -3 polarity [14]. It has been widely reported that circRNAs exhibit stage-dependent expression patterns in testis development of pig, sheep, and bovine [15][16][17][18], which indicates that circRNAs might play crucial regulatory roles in testis development and spermatogenesis. Existing literature has demonstrated that circRNAs participate in diverse biological processes through acting as an endogenous sponge of miRNA, including rolling circle translation, post-transcriptional regulation, circRNA-derived pseudogenes, and splicing interference [19,20].…”

Section: Introductionmentioning

confidence: 99%

circBTBD7 Promotes Immature Porcine Sertoli Cell Growth through Modulating miR-24-3p/MAPK7 Axis to Inactivate p38 MAPK Signaling Pathway

Bian

Chen

Wang

et al. 2021

IJMS

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Sertoli cells are the crucial coordinators to guarantee normal spermatogenesis and male fertility. Although circular RNAs (circRNAs) exhibit developmental-stage-specific expression in porcine testicular tissues and have been thought of as potential regulatory molecules in spermatogenesis, their functions and mechanisms of action remain largely unknown, especially in domestic animals. A novel circBTBD7 was identified from immature porcine Sertoli cells using reverse transcription PCR, Sanger sequencing, and fluorescence in situ hybridization assays. Functional assays illustrated that circBTBD7 overexpression promoted cell cycle progression and cell proliferation, as well as inhibited cell apoptosis in immature porcine Sertoli cells. Mechanistically, circBTBD7 acted as a sponge for the miR-24-3p and further facilitated its target mitogen-activated protein kinase 7 (MAPK7) gene. Overexpression of miR-24-3p impeded cell proliferation and induced cell apoptosis, which further attenuated the effects of circBTBD7 overexpression. siRNA-induced MAPK7 deficiency resulted in a similar effect to miR-24-3p overexpression, and further offset the effects of miR-24-3p inhibition. Both miR-24-3p overexpression and MAPK7 knockdown upregulated the p38 phosphorylation activity. The SB202190 induced the inhibition of p38 MAPK pathway and caused an opposite effect to that of miR-24-3p overexpression and MAPK7 knockdown. Collectively, circBTBD7 promotes immature porcine Sertoli cell growth through modulating the miR-24-3p/MAPK7 axis to inactivate the p38 MAPK signaling pathway. This study expanded our knowledge of noncoding RNAs in porcine normal spermatogenesis through deciding the fate of Sertoli cells.

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Strand-specific RNA sequencing in pig testes identifies developmentally regulated genes and circular RNAs

Cited by 8 publications

References 41 publications

Biological functions of circRNAs and their progress in livestock and poultry

Biological functions of circRNAs and their progress in livestock and poultry

miR-222 Suppresses Immature Porcine Sertoli Cell Growth by Targeting the GRB10 Gene Through Inactivating the PI3K/AKT Signaling Pathway

circBTBD7 Promotes Immature Porcine Sertoli Cell Growth through Modulating miR-24-3p/MAPK7 Axis to Inactivate p38 MAPK Signaling Pathway

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