Strategies for Bioprospecting Microalgae for Potential Commercial Applications

Barclay, William R.; Apt, Kirk E.

doi:10.1002/9781118567166.ch4

Cited by 18 publications

(15 citation statements)

References 47 publications

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“…In the case of algae, mechanical dewatering is only viable to about 80% dry matter and thermal drying requires high amounts of energy [18]. Consequently, particularly the processing of wet feedstocks is a key technology to enable algaesuccess on a large scale [19,20].…”

Section: Introductionmentioning

confidence: 99%

Extraction of common microalgae by liquefied dimethyl ether: influence of species and pretreatment on oil yields and composition

Bauer

Konnerth

Kruse

2020

Biomass Conv. Bioref.

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Liquefied dimethyl ether (DME) is regarded as a promising, green solvent for biomass lipid extractions. It is non-toxic, applicable to wet feedstocks, and allows easy product separation by pressure reduction. Yet, knowledge about its usability in combination with oleaginous microalgae is limited. In the current work, four common microalgae and cyanobacteria species were used to study DME extraction characteristics: Arthrospira platensis, Nannochloropsis gaditana, Phaeodactylum tricornutum, and Scenedesmus almeriensis. Dried samples were subjected to a batch DME extraction and compared to a standard chloroform/methanol procedure. To evaluate the influence of pretreatment, particle size distributions of two different milling sequences (knife- and cryo-milling) and the resulting effects on DME extraction and oil composition were addressed. Additionally, an algae washing procedure was tested. DME extractions resulted in oil yields of 0.5–2.7% of dry mass (equal to 5–19% of total lipids) without further pretreatment. Cryo-milling reduced median particle sizes by 25–87% and simultaneously increased lipid yields to 1.7–5.6% of dry mass (17–50% of total lipids). Phaeodactylum tricornutum showed the highest extraction efficiency with DME, combined with a favorable fatty acid profile. Although being most affected by the additional milling pretreatment, Arthrospira platensis performed worst in both scenarios. DME extracted oils were generally characterized by enhanced contents of C14:0, C16:0, and C16:1 fatty acids. However, relative abundances were strongly influenced by the properties of the tested algae species. The additional cryo-milling pretreatment affected fatty acid compositions by increasing the shares of potentially valuable polyunsaturated fatty acids.

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Section: Introductionmentioning

confidence: 99%

Extraction of common microalgae by liquefied dimethyl ether: influence of species and pretreatment on oil yields and composition

Bauer

Konnerth

Kruse

2020

Biomass Conv. Bioref.

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“…Algae and cyanobacteria are some of the main biological protagonists that drive many global biogeochemical cycles, being directly responsible for over half of current planetary productivity and geologically for many fossil-fuel resources 4 . Their high conversion of solar energy into biomass, capacity to grow in saline or hypersaline environments, ability to metabolise industrial and domestic waste streams (including CO 2 flue gases and wastewater), combined with the diverse and arguably untapped range of biochemicals they can synthesise 5 6 makes algae and cyanobacteria attractive bioprospecting targets 7 8 . In this regard, culture collections that curate microalgae and cyanobacteria, whether public or private, have proven to be fruitful and convenient biological reservoirs for bioprospecting 8 9 .…”

mentioning

confidence: 99%

Proteomic-based biotyping reveals hidden diversity within a microalgae culture collection: An example using Dunaliella

Emami

Hack

Nelson

et al. 2015

Sci Rep

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Accurate and defendable taxonomic identification of microalgae strains is vital for culture collections, industry and academia; particularly when addressing issues of intellectual property. We demonstrate the remarkable effectiveness of Matrix Assisted Laser Desorption Ionisation Time of Flight Mass Spectrometry (MALDI-TOF-MS) biotyping to deliver rapid and accurate strain separation, even in situations where standard molecular tools prove ineffective. Highly distinctive MALDI spectra were obtained for thirty two biotechnologically interesting Dunaliella strains plus strains of Arthrospira, Chlorella, Isochrysis, Tetraselmis and a range of culturable co-occurring bacteria. Spectra were directly compared with genomic DNA sequences (internal transcribed spacer, ITS). Within individual Dunaliella isolates MALDI discriminated between strains with identical ITS sequences, thereby emphasising and enhancing knowledge of the diversity within microalgae culture collections. Further, MALDI spectra did not vary with culture age or growth stage during the course of the experiment; therefore MALDI presents stable and accurate strain-specific signature spectra. Bacterial contamination did not affect MALDI’s discriminating power. Biotyping by MALDI-TOF-MS will prove effective in situations wherein precise strain identification is vital, for example in cases involving intellectual property disputes and in monitoring and safeguarding biosecurity. MALDI should be accepted as a biotyping tool to complement and enhance standard molecular taxonomy for microalgae.

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“…al. 2000) o para consumo humano (Sukenik, 1999;Barclay y Apt, 2013). Por consiguiente, estudiar los parámetros fisicoquímicos y contenido de nutrientes que mejoren el comportamiento reproductivo y calidad nutricional de las microalgas, contribuye al fortalecimiento de la bioeconomía acuícola (Fernández et al, 2018).…”

Section: Unan-leónunclassified

“…Es más, que esta microalga puede continuar reproduciéndose posiblemente a la capacidad de protección celular frente al estrés que presenta la vía fotorrespiratoria (Xiao et. Al., 2013;Barclay y Apt, 2013) debido a la acumulación de grandes cantidades del enzima rubisco y que los bioelementos invertidos en su síntesis pueden ser recuperados por medio de las rutas catabólicas de rubisco que se activan cuando se produce una decadencia funcional durante la senescencia natural o inducida por estrés, o en una situación de carencia de nutrientes (Ferreira et. al., 2000;Wei et.…”

Section: Unan-leónunclassified

Capacidad reproductiva de Nannochloropsis oculata en diferentes concentraciones de salinidad y fertilizante: Una contribución a la Bioeconomía acuícola

Osorio-Urtecho

Lumbi-Ortega

Hsieh³

et al. 2020

(Rev. iberoam. bioecon. cambio clim.)

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El objetivo del presente estudio fue evaluar el ritmo reproductivo de Nannochloropsis oculata usando dos baterías experimentales y aplicando dosis únicas de 0.32 ‰ y 1 ‰ de F/2 Guillard, respectivamente; en salinidades de 33 ‰, 25 ‰, 20 ‰, 15 ‰, 10 ‰ y 5 ‰, periodos de 10 y 23 días y temperatura de 25 ºC. En un primer experimento, con 0.32 ‰ de F/2 Guillard, N. oculata presentó mayor número de cel/ml en salinidades de 33 ‰ y 25 ‰, a los 8 días de estudio, denotando la afinidad de N. oculata de reproducirse con mayor velocidad en ese rango de salinidad. La importancia del uso de F/2 Guillard fue observada usando 1‰ debido a que la microalga presenta similar número de cel/ml (P≤0.05) en concentraciones salinas de 33 ‰, 25 ‰, 20 ‰ y 15 ‰ y similar concentración a las microalgas cultivadas con 0.32 ‰ de F/2 Guillard en salinidades de 33 ‰ y 25 ‰. La capacidad de N. oculata de reproducirse en concentraciones salinas de 33 ‰, 25 ‰ y 20 ‰ y niveles bajos de nutrientes fue testado en un segundo experimento donde los resultados muestran similar número de cel/ml entre los tratamientos salinos, en ambas baterías, a los 23 días de cultivo. Nuestros resultados sugieren que N. oculata puede reproducirse con escaza concentración de nutrientes y que su metabolismo puede estar siendo mediado por la catálisis de algunas proteínas como rubisco que sirve como una importante reserva de nitrógeno, azufre y carbono.

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Strategies for Bioprospecting Microalgae for Potential Commercial Applications

Cited by 18 publications

References 47 publications

Extraction of common microalgae by liquefied dimethyl ether: influence of species and pretreatment on oil yields and composition

Extraction of common microalgae by liquefied dimethyl ether: influence of species and pretreatment on oil yields and composition

Proteomic-based biotyping reveals hidden diversity within a microalgae culture collection: An example using Dunaliella

Capacidad reproductiva de Nannochloropsis oculata en diferentes concentraciones de salinidad y fertilizante: Una contribución a la Bioeconomía acuícola

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