Strategies for the isolation and identification of trypanocidal compounds from the Rutales

Vieira, Paulo C.; Mafezoli, Jair; Pupo, Mônica Tallarico; Fernandes, João B.; Silva, Maria Fátima das Graças Fernandes da; Albuquerque, Sérgio de; Oliva, Glaucius; Pavão, Fernando

doi:10.1351/pac200173030617

Cited by 40 publications

(39 citation statements)

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“…However, no such result has been found for T. cruzi-gGAPDH. Thus, the ability of the ruthenium NO donors trans- [Ru(NO) 3 to inhibit the catalytic activity of T. cruzi-gGAPDH has been investigated by using a previously reported procedure (52). No significant inhibitory activity was found when the enzyme was assayed in these compounds in concentrations up to 350 M, not even in the presence of reducing agents such as ascorbic acid and cysteine.…”

Section: Discussionmentioning

confidence: 99%

Experimental Chemotherapy againstTrypanosoma cruziInfection Using Ruthenium Nitric Oxide Donors

Silva

Pavanelli

Pereira

et al. 2009

Antimicrob Agents Chemother

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Section: Discussionmentioning

confidence: 99%

Experimental Chemotherapy againstTrypanosoma cruziInfection Using Ruthenium Nitric Oxide Donors

Silva

Pavanelli

Pereira

et al. 2009

Antimicrob Agents Chemother

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“…In the same way, some Pilocarpus-obtained compounds also act as antidotes on intoxication by Datura stramonium (Salyi;Abonyi, 1994), induce spittling (Kempf, 1999) and have in vitro action against Pseudomonas aeruginosa, Staphylococcus aereus and Trypanosoma cruzi (Santos et al, 1997;Vieira et al, 2001;Mafezoli et al, 2000;Pavão et al, 2002). Essential oil extracted from a variety of host plants (Guenter, 1972) have been recently reported on account of their effects on the development and behavior of insects as Trialeurodes vaporariorum (Choi et al, 2003), Tribolium confusum (Tunc;Erler, 2003), mosquito species (Tuetun et al, 2004;Costa et al, 2005;Pimenta et al, 2006), Triatoma infestans and Rhodnius neglectus (Fournet et al, 1996;Laurent et al, 1997;Vilaseca et al, 2004), Pediculus humanus (Cestari et al, 2004), Oxyops vitiosa (Wheeler, 2005), Meligethes aeneus (Mauchline et al, 2005) and Lobesia botrana (Katerinopoulos et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Effects of the essential oil obtained from Pilocarpus spicatus Saint-Hilaire (Rutaceae) on the development of Rhodnius prolixus nymphae

Mello¹,

Uzeda²,

Bernardino³

et al. 2007

Rev. bras. farmacogn.

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RESUMO: "Efeitos do óleo essencial de Pilocarpus spicatus Saint-Hilaire (Rutaceae) no desenvolvimento de ninfas de Rhodnius prolixus". Bioensaios contra ninfas de 5º estádio de Rhodnius prolixus foram conduzidos utilizando-se óleo essencial de Pilocarpus spicatus extraído por hidrodestilação. Os principais resultados podem ser resumidos como se segue: (i) altos níveis de toxicidade e paralisia associados à discreta inibição da muda foram induzidos pela aplicação tópica de 0,5 μL ou 1,0 μL do óleo essencial por inseto; (ii) fagoinibição parcial, altos níveis de inibição da muda, período intermuda prolongado e alto número de insetos paralisados mas ausência de toxicidade foram observados após tratamento oral com 5,0 μL ou 10 μL de óleo essencial de P. spicatus por mL de sangue ingerido. A importância destes resultados em relação a eventos biológicos relevantes em R. prolixus é aqui discutida. Unitermos: Rhodnius prolixus, Pilocarpus spicatus, óleo essencial.ABSTRACT: Bioassays against fi fth-instar nymphae of Rhodnius prolixus were conducted with essential oil of Pilocarpus spicatus extracted by hydrodistillation. The main results may be summarized as follows: (i) high levels of toxicity and paralysis together with discrete moulting inhibition were caused by topical application of either 0.5 μL or 1.0 μL per insect of the crude essential oil; (ii) partial fagoinhibition, high moulting inhibition, prolonged intermoulting period and high number of paralyzed insects, but no toxicity were observed after oral treatment using either 5 μL or 10 μL of Pilocarpus spilcatus essential oil per mL of ingested blood meal. The importance of these results in relation to the relevant biological events in R. prolixus is herein discussed.

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“…Estas muestras más los respectivos controles negativo (sin extracto) y positivo (incluyendo parásitos muertos por calor), fueron colocados a 4ºC por 24 horas (Vieira et al, 2001), después de lo cual la viabilidad de los parásitos fue determinada por medio del azul tripano, técnica ampliamente conocida. La actividad antiparasitaria fue determinada estableciendo como parámetro la presencia de más del 50% de promastigotos teñidos.…”

Section: Ensayos In Vitrounclassified

Actividad contra Leishmania sp. (Kineplastida: Trypanosomatidae) de plantas en una Reserva Biológica de Costa Rica

Chinchilla-Carmona

Valerio-Campos

Sánchez-Porras

et al. 2014

RBT

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Anti-leishmanial activity in plants from a Biological Reserve of Costa Rica. Leishmaniosis is an important human disease very difficult to treat. For this reason, many researchers in the world have been looking for anti-leishmanial chemical components present in several plant species. In Costa Rica, since no studies have been done in this field, this work aimed at the search of active chemical components in local plants that may have an activity against Leishmania sp. A total of 67 plants were selected from the Alberto Manuel Brenes Biological Reserve (REBAMB). For these collected plants, fresh or dried hidroalcoholic extracts of root, stem, mature or young leaves, flowers, and immature or mature fruits, were prepared under conventional methods. All extracts were tested for their effect against a strain of Leishmania (OCR with known characteristics). Firstly, by presumptive tests, we selected only those with some activity, and then, more specific studies were done to determine the IC 50 in µg/mL; a promising plant was considered only if at least one of its parts presented an IC 50 <100µg/mL. Under this parameter, the following active plants were obtained and their lowest and highest (23.4 and 97.5). Although the parasitic effect of fresh or dried extracts were almost similar, the fresh material slightly showed better results. That anti-parasitic effect occurred in one or more than four parts of the plant. Most of the active extracts did not produce lysis and aglutination which indicates a low toxicity. Since the species studied are different from those analyzed by other authors, we discuss the importance of these new findings, in relation to the new scientific knowledge, and the possible use of these plants as a leishmaniosis treatment. Rev. Biol. Trop. 62 (3): 1229-1240. Epub 2014 September 01.

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Strategies for the isolation and identification of trypanocidal compounds from the Rutales

Cited by 40 publications

References 0 publications

Experimental Chemotherapy againstTrypanosoma cruziInfection Using Ruthenium Nitric Oxide Donors

Experimental Chemotherapy againstTrypanosoma cruziInfection Using Ruthenium Nitric Oxide Donors

Effects of the essential oil obtained from Pilocarpus spicatus Saint-Hilaire (Rutaceae) on the development of Rhodnius prolixus nymphae

Actividad contra Leishmania sp. (Kineplastida: Trypanosomatidae) de plantas en una Reserva Biológica de Costa Rica

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