AQUJ 2021
DOI: 10.47874/2021p3
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Strategy for DNA extraction and detection from insect pests in stored home grain samples RESEARCH

Abstract: Stored grains are subjected to infestations with more than 60 species of insects, that responsible for millions of dollars’ loss and cause several health problems including allergies and gastrointestinal disorders. Traditional detection techniques are laborious, expensive and not sensitive to detect insect contamination at the egg and larvae stages. Therefore, alternative methods are needed for rapid and sensitive detection. One obvious approach is to develop a molecular approach utilizing genetic information … Show more

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“…The PCR was carried out in a Veriti 96-well Thermal cycler (Applied Biosystems, Singapore) using a reaction volume of 25 µL containing 2 µL of DNA (50 ng), 1 µL of each primer (20 pmol) and a PuReTaq Ready-To-Go PCR Bead (Cytiva, Global Life Sciences Solutions Operations UK Ltd., Little Chalfont, Buckinghamshire, UK) dissolved in 21 µL of sterile distilled water (SDW). The PCR cycles and conditions were according to Abbasi et al [35]. The sequence of the PCR product was determined (Macrogen Inc., Gangnam-gu, Seoul, Republic of Korea), and the nucleotide sequences were compared with reference sequences in the GenBank (http://www.ncbi.nlm.nih.gov; accessed on 30 October 2023) using the BLASTN tool.…”
Section: Molecular Identification Of Rice Weevilmentioning
confidence: 99%
“…The PCR was carried out in a Veriti 96-well Thermal cycler (Applied Biosystems, Singapore) using a reaction volume of 25 µL containing 2 µL of DNA (50 ng), 1 µL of each primer (20 pmol) and a PuReTaq Ready-To-Go PCR Bead (Cytiva, Global Life Sciences Solutions Operations UK Ltd., Little Chalfont, Buckinghamshire, UK) dissolved in 21 µL of sterile distilled water (SDW). The PCR cycles and conditions were according to Abbasi et al [35]. The sequence of the PCR product was determined (Macrogen Inc., Gangnam-gu, Seoul, Republic of Korea), and the nucleotide sequences were compared with reference sequences in the GenBank (http://www.ncbi.nlm.nih.gov; accessed on 30 October 2023) using the BLASTN tool.…”
Section: Molecular Identification Of Rice Weevilmentioning
confidence: 99%