Strategy for the detection of Helicobacter species by amplification of 16S rRNA genes and identification of H. felis in a human gastric biopsy

Germani, Y; Dauga, Catherine; Duval, Philippe; Huerre, Michel; Levy, M; Pialoux, Gilles; Sansonetti, P; Grimont, Patrick A. D.

doi:10.1016/s0923-2508(97)81587-2

Cited by 95 publications

(87 citation statements)

References 41 publications

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“…11 We considered H. pylori infection when at least three (16S rRNA, glmM, and ureA) of the four genes were present, and non-H. pylori helicobacters (NHPHs) infection when only 16S rRNA gene was present. 16 Helicobacter pylori in the rural population was found positive in 87.2% (34/39) of patients, whereas it was found positive in 55.0% (39/71) of subjects living in the urban area. Helicobacter pylori infection in the rural mestizo population *Address correspondence to Monica Contreras, Laboratorio de Fisiología Gastrointestinal, Centro de Biofísica y Bioquímica.…”

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confidence: 86%

Helicobacter pylori Infection in Rural and Urban Dyspeptic Patients from Venezuela

Contreras

Fernández-Delgado

Reyes

et al. 2015

The American Society of Tropical Medicine and Hygiene

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Abstract. The goal of this work was to assess the Helicobacter pylori prevalence in a rural mestizo population and compare it to an urban population from Venezuela. The study was performed in gastric juice samples of 71 dyspeptic patients from Caracas (urban) and 39 from Tucupita (rural), in the Orinoco Delta region. Helicobacter pylori was detected by amplification of 16S rRNA, glmM, and ureA genes in 55.0% patients from urban and 87.2% from rural populations. cagA was found positive in 51% and 62% urban and rural patients, respectively. Non-H. pylori Helicobacter species were not detected in the urban population, but was found in 7.7% of patients in the rural study site. Frequency values of the 16S rRNA, glmM, and ureA genes were higher in the rural population. The odds ratio for each gene was 15.18 for 16S rRNA, 2.34 for glmM, 2.89 for ureA, and 1.53 cagA, showing significant differences except for cagA when gene frequency was compared in both populations. These results demonstrate a higher frequency of H. pylori and gastric non-H. pylori Helicobacter infection in a rural mestizo population with low hygienic standards as compared with city dwellers, representing a potential risk for the development of gastroduodenal diseases.

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confidence: 86%

Helicobacter pylori Infection in Rural and Urban Dyspeptic Patients from Venezuela

Contreras

Fernández-Delgado

Reyes

et al. 2015

The American Society of Tropical Medicine and Hygiene

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“…Biopsy samples were taken once per week from gastric mucosa for histology and culture. Gastric secretory tests were performed during week 3 and then repeated in weeks 5,7,9,12,24, and 42. Pentagastrin-induced gastric acid secretion was determined in weeks 12 and 42.…”

Section: H Pylori Infectionmentioning

confidence: 99%

“…The plates were incubated in an anaerobic jar with a microaerophilic gas-generating kit for 2 days at 37°C. Bacteria that tested positive for urease and oxidase were identified on the basis of PCR analysis, which showed 16S ribosomal DNA sequences to be Ͼ98% identical to those of H. felis or H. heilmannii (22)(23)(24). In most cases, Helicobacter-like organisms were identified to be H. felis, and H. pylori was not detected in any of these animals before experimental infection.…”

Section: Bacteriamentioning

confidence: 99%

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Putative effect ofHelicobacter pyloriand gastritis on gastric acid secretion in cat

Sobhani

Cañedo

Alchepo

et al. 2002

American Journal of Physiology-Gastrointestinal and Liver Physi

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“…Presence of canine Gastric Helicobacter-like organisms in 29 of 32 stray dogs (90.5%) was confirmed by Light [22] (f ): AAC GAT GAA GCT TCT AGC TTG CTA (r): GTG CTT ATT CGT GAG ATA CCG TCA T 399 bp ure B gene of H. felis Germani [22] (f ): GTG AAG CGA CTA AAG ATA AAC AAT (r): GCA CCA AAT CTA ATT CAT AAG AGC 241 bp ureB gene of H. heilmannii Neiger [23] (f ): GGG CGA TAA AGT GCG CTT G (r): CTG GTC AAT GAG AGC AGG 580 bp ureC gene of H. pylori Labinge [24] (f ): GGA TAA GCT TTT AGG GGT GTT AGG GG (r): GCT TAC TTT CTA ACA CTA ACG AGG 294 bp ureB gene of H. bizzozeronii Neiger [23] ( …”

Section: Impression Smearsmentioning

confidence: 85%

Assessment of Gastric Helicobacter spp. in Fresh Gastric Samples of Naturally Infected Dogs by Scanning Electron Microscopy

Asl¹,

Amanizad²,

Partovi³

et al. 2014

Kafkas Univ Vet Fak Derg

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SummaryDifferent species of gastric Helicobacter-Like Organisms (GHLO) reported from dogs' stomach. The aim of present study was to morphological evaluation of gastric Helicobacter spp. in fresh gastric samples of naturally infected dogs. Thirty two gastric samples of the stray dog were taken at necropsy. The specimens were used for rapid urease test, light microscopy, scanning electron microscopy (SEM) and polymerase chain reaction (PCR). Light microscopy examination confirmed the presence of GHLO in 90.5% of stray dogs. 87.5% and 94% of gastric samples were positive in rapid urease test and PCR, respectively. Four distinguishable Helicobacter organisms were confirmed by SEM. Three strains of these organisms were inditified as H. felis, candidatus H. heilmanii and H. bizzozeronii because of their apparent morphological differences and PCR results. The last strain of these bacteria was not distinguishable with routine studies. The large-scale studies with fast and simple recognition methods are recommended to confirm the different types of canine gastric Helicobacter. The results of present study showed further investigation in canine GHLO is required because new species of Helicobacter reported.

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Strategy for the detection of Helicobacter species by amplification of 16S rRNA genes and identification of H. felis in a human gastric biopsy

Cited by 95 publications

References 41 publications

Helicobacter pylori Infection in Rural and Urban Dyspeptic Patients from Venezuela

Helicobacter pylori Infection in Rural and Urban Dyspeptic Patients from Venezuela

Putative effect ofHelicobacter pyloriand gastritis on gastric acid secretion in cat

Assessment of Gastric Helicobacter spp. in Fresh Gastric Samples of Naturally Infected Dogs by Scanning Electron Microscopy

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