Strawberry Breeding and Varieties: Situation and Perspectives

Faedi, W.; Mourgues, F.; Rosati, Carlo

doi:10.17660/actahortic.2002.567.1

Cited by 51 publications

(31 citation statements)

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“…In addition, juvenile leaves of Sweet Charlie reached the highest number of shoots per explant (4 shoots /explant) compared with other cultivars under analysis. These results are in consensus with previous reports, 38,39 indicating that chemical composition of the media is one of the factors determining the success of in vitro. In addition, selection of the proper hormones is the key to regeneration success in strawberries.…”

Section: Adventitious Shoots Formationsupporting

confidence: 93%

Improved regeneration and transformation protocols for three strawberry cultivars

et al. 2013

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strawberry (Fragaria × ananassa) is an economically important soft fruit crop with polyploid genome, which makes the breeding of new cultivars difficult. simple and efficient methods for transformation and regeneration are required for cultivars improvement in strawberries. In the present study, adventitious shoot regeneration has been investigated in three cultivated strawberry plants, i.e., Festival, sweet charlie, and Florida via direct organogenesis using the in vitro juvenile leaves as explants. explants were collected after sub-culturing on a propagation medium composed of Ms supplemented with 0.5 mg/l Ba; 0.1 mg/l Ga3 and 0.1 mg/l IBa. To select the suitable organogenesis, the explants of the three cultivars were cultured on Ms medium supplemented with different concentrations of TDZ (1, 2, 3, and 4 mg/l), then incubated at a temperature of 22 °c ± 2. Medium containing 2 mg/l TDZ revealed the best regeneration efficiency with the three cultivars (72% for Festival and 73% for sweet charlie and Florida). after 4 weeks the produced shoots were cultured on Ms medium with different concentrations of Ba and Kin to enhance shoot elongation. Results showed that the medium containing 1.5 mg/l Ba and 0.5 mg/l Kin revealed highest elongation efficiency (88% and 94%) for Festival and sweet charlie, respectively. On the other hand, medium media containing 1.5 mg/l Ba and 0.1 mg/l Kin showed highest elongation efficiency (90%) in Florida. elongated shoots were successfully rooted on Ms medium containing 1.5 mg/l Naa. Furthermore, transformation of the two cultivars, Festival and sweet charlie, has been established via Agrobacterium strain LBa44404 containing the plasmid pIsV2678 with gus-intron and bar genes. Three days post-cocultivation, GUs activity was screened using the histochemical assay. The results showed 16% and 18% of the tested plant materials had changed into a blue color for Festival and sweet charlie, respectively. Out of 120 explants only 13 shoots were developed on bialaphos medium for each cultivar, representing 10.8% bialaphos-resistant strawberry shoot. The presence of both the genes bar and uid a was detected by PcR and Northern, giving a transformation efficiency of 5%.

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Section: Adventitious Shoots Formationsupporting

confidence: 93%

Improved regeneration and transformation protocols for three strawberry cultivars

et al. 2013

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“…plant genotype, mineral formulation, type and concentration of cytokinin in the medium and the number of subcultures. It is generally recommended not to exceed four to five subcultures to avoid loss of trueness-to type of the propagated material (Faedi et al, 2002). This technique is useful in case of the introduction of new cultivars.…”

Section: Introductionmentioning

confidence: 99%

Study of Shoot Multiplication of Strawberry (Fragaria ananassa)

Zobayer

Prodhan

Sikdar

et al. 2013

Int. J. Agril. Res. Innov. & Tech.

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Effects of different hormonal concentration on multiple shoot formation of RABI Strawberry-3 were studied. Where the explants were runner tips that cultured on MS basal medium supplemented with 6-Benzyl Adenine and Kinetin. The maximum percentage 80% of shoot was obtained from Benzyl Adenine concentrations of 0.5 mg/l and 1.0 mg/l whereas Kinetin concentrations were 1.5 mg/l and 2.0 mg/l separately. More promising result was found by combining two impressive concentrations of each hormone. When MS basal media was supplemented with Kinetin 2.0mg/l + Benzyl Adenin A 0.5 mg/l, Kinetin 1.5 mg/l + Benzyl Adenine 0.5 mg/l and Kinetin 1.5 mg/l + Benzyl Adenine 1.0 mg/l respectively then 100% shoots were obtained.

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“…Similar to these Solanaceae crops, strawberry plants are also included in several biotechnological breeding programmes (Faedi et al 2002). During the 1990s, reliable protocols for the genetic transformation of strawberry were established for several commercial cultivars (Barceló et al 1998;James et al 1990;Mathews et al 1995).…”

Section: Introductionmentioning

confidence: 99%

TheCaMV 35S promoter is highly active on floral organs and pollen of transgenic strawberry plants

et al. 2004

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We have evaluated the expression of the reporter beta-glucuronidase (GUS) gene driven by the cauliflower mosaic virus 35S (CaMV 35S) promoter in flowers and pollen from 14 independent transgenic strawberry lines. Of the 14 lines evaluated, 13 (92.8%) showed GUS activity--as estimated by the histochemical GUS assay--in some floral organs, with expression being most common in the flower stem, sepals, petals, ovary and stigma. Ten of these thirteen transgenic lines (77%) showed GUS activity in pollen, although the percentages of positive pollen per flower varied greatly among the different lines. A study of the GUS expression during pollen maturation showed that the (CaMV 35S) promoter showed low expression in pollen from flower buds before anthesis but was activated in mature pollen following anther dehiscence. The percentages of pollen grains that showed GUS activity ranged from 2.1% to 46.3%. These percentages were similar or even higher when mature pollen was stored dry at room temperature for 2 weeks. After 5 weeks of storage, the percentages of GUS-positive pollen decreased in two of the six lines analysed but remained at similar values in the other four lines. GUS activity was also measured in protein extracts of mature pollen by means of the fluorometric GUS assay, with the values obtained ranging from 3.8 micromol MU mg protein(-1) h(-1) to 0.26 micromol MU mg protein(-1) h(-1). Contrary to the generally held view that the CaMV 35S promoter is virtually silent in pollen, we conclude that it is highly expressed in transgenic strawberry pollen.

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Strawberry Breeding and Varieties: Situation and Perspectives

Cited by 51 publications

References 0 publications

Improved regeneration and transformation protocols for three strawberry cultivars

Improved regeneration and transformation protocols for three strawberry cultivars

Study of Shoot Multiplication of Strawberry (Fragaria ananassa)

TheCaMV 35S promoter is highly active on floral organs and pollen of transgenic strawberry plants

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