(Strept)avidin as a template for ligands other than biotin: An overview

Hytönen, Vesa P.

doi:10.1016/bs.mie.2019.10.029

Cited by 5 publications

(1 citation statement)

References 34 publications

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“…Up to 50 % of biotin consumption by hens is deposited in the eggs. The high affinity of avidin for biotin is so strong that this characteristic has converted the pair into a widely used tool in protein science and biotechnology [ 33 ]. The non-covalent association ( K m ) of biotin with avidin is in the order of 10 −15 M, with a very slow dissociation rate.…”

Section: Resultsmentioning

confidence: 99%

A Tar aspartate receptor and Rubisco-like protein substitute biotin in the growth of rhizobial strains

et al. 2022

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Biotin is a key cofactor of metabolic carboxylases, although many rhizobial strains are biotin auxotrophs. When some of these strains were serially subcultured in minimal medium, they showed diminished growth and increased excretion of metabolites. The addition of biotin, or genetic complementation with biotin synthesis genes resulted in full growth of Rhizobium etli CFN42 and Rhizobium phaseoli CIAT652 strains. Half of rhizobial genomes did not show genes for biotin biosynthesis, but three-quarters had genes for biotin transport. Some strains had genes for an avidin homologue (rhizavidin), a protein with high affinity for biotin but an unknown role in bacteria. A CFN42-derived rhizavidin mutant showed a sharper growth decrease in subcultures, revealing a role in biotin storage. In the search of biotin-independent growth of subcultures, CFN42 and CIAT652 strains with excess aeration showed optimal growth, as they also did, unexpectedly, with the addition of aspartic acid analogues α- and N-methyl aspartate. Aspartate analogues can be sensed by the chemotaxis aspartate receptor Tar. A tar homologue was identified and its mutants showed no growth recovery with aspartate analogues, indicating requirement of the Tar receptor in such a phenotype. Additionally, tar mutants did not recover full growth with excess aeration. A Rubisco-like protein was found to be necessary for growth as the corresponding mutants showed no recovery either with high aeration or aspartate analogues; also, diminished carboxylation was observed. Taken together, our results indicate a route of biotin-independent growth in rhizobial strains that included oxygen, a Tar receptor and a previously uncharacterized Rubisco-like protein.

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Section: Resultsmentioning

confidence: 99%

A Tar aspartate receptor and Rubisco-like protein substitute biotin in the growth of rhizobial strains

et al. 2022

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Ordered proteins and structure–function relationship: a classical view

Gupta,

Tripathi,

Uversky

2025

The Three Functional States of Proteins

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The use of phage display systems to combat infectious diseases in poultry: diagnostic, vaccine, and therapeutic approaches

Grabowski

Pierzynowska

Gaffke

et al. 2022

Journal of Applied Microbiology

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Development of molecular biology and understanding structures and functions of various biological molecules and entities allowed to construct various sophisticated tools for different biotechnological, medical, and veterinary applications. One of them is the phage display technology, based on the possibility to create specific bacteriophages bearing fusion genes, which code for fusion proteins consisting of a phage coat protein and a peptide of any amino acid sequence. Such proteins retain their biological functions as structural elements of phage virions while exposing foreign peptide sequences on their surfaces. Genetic manipulations allow to construct phage display libraries composed of billions of variants of exposed peptides; such libraries can be used to select peptides of desired features. Although the phage display technology has been widely used in biotechnology and medicine, its applications in veterinary and especially in poultry science were significantly less frequent. Nevertheless, many interesting discoveries have been reported also in the latter field, providing evidence for a possibility of effective applications of phage display-related methods in developing novel diagnostic tools, new vaccines, and innovative potential therapies dedicated to poultry. Especially, infectious diseases caused by avian viruses, bacteria, and unicellular eukaryotic parasites were investigated in this field. These studies are summarized and discussed in this review, with presentation of various possibilities provided by different phage display systems in development of useful and effective products facilitating management of the problem of infectious diseases of poultry.

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(Strept)avidin as a template for ligands other than biotin: An overview

Cited by 5 publications

References 34 publications

A Tar aspartate receptor and Rubisco-like protein substitute biotin in the growth of rhizobial strains

A Tar aspartate receptor and Rubisco-like protein substitute biotin in the growth of rhizobial strains

Ordered proteins and structure–function relationship: a classical view

The use of phage display systems to combat infectious diseases in poultry: diagnostic, vaccine, and therapeutic approaches

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