Streptavidin arrays as supramolecular architectures in surface-plasmon optical sensor formats

Knoll, Wolfgang; Zizlsperger, Manfred; Liebermann, Thorsten; Arnold, Stefan; Badia, Antonella; Liley, Martha; Piscevic, Darko; Schmitt, Franz‐Josef; Spinke, J.

doi:10.1016/s0927-7757(99)00331-3

Cited by 101 publications

(116 citation statements)

References 31 publications

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“…This sensitivity is close to the one reached by surface plasmon resonance spectroscopy (SPR) (21,33). More recently, Knoll et al demonstrated that sensitivity could be even enhanced by combining field-enhancement SPR with fluorescence detection, using, for instance a biotinylated chromophore (23). Fluorescence SCHEME 2.…”

Section: Sensitivity Of the Technique And Comparison With Other Transsupporting

confidence: 60%

“…Previously described methods of formation of SAMs of biotin molecules on gold or silicon substrates consisted either in the covalent coupling to previously chemisorbed ω-functionalized thiolates (19)(20)(21)(22) or in the direct chemisorption of biotinylated thiols or disulfides (23,24). Mixed biotinylated SAMs, consisting in a biotinylated alkylthiol coadsorbed with an hydroxy-terminated second thiol, have also been successfully utilized to monitor the structure and order of the reactive adsorbed layer (25)(26)(27).…”

Section: Formation Of a Biotin Layer Onto The Gold Surfacesmentioning

confidence: 99%

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Molecular Recognition of Avidin on Biotin-Functionalized Gold Surfaces Detected by FT-IRRAS and Use of Metal Carbonyl Probes

Yam

Pradier²,

Salmain³

et al. 2002

Journal of Colloid and Interface Science

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Section: Sensitivity Of the Technique And Comparison With Other Transsupporting

confidence: 60%

Section: Formation Of a Biotin Layer Onto The Gold Surfacesmentioning

confidence: 99%

Molecular Recognition of Avidin on Biotin-Functionalized Gold Surfaces Detected by FT-IRRAS and Use of Metal Carbonyl Probes

Yam

Pradier²,

Salmain³

et al. 2002

Journal of Colloid and Interface Science

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“…For the layer-by-layer deposition, we firstly modified the gold surface with a mixed thiol self-assembled monolayer (SAM) [11]. The freshly evaporated gold was incubated overnight in the 9:1 mixture of 11-mercaptoundecan-1-ol and 11-mercapto-(8-biotinamido-4,7,dioxyaoctyl-)-undecanoylamid (total concentration 0.5 mM in ethanol), successively washed with ethanol and deionized water, dried with a stream of nitrogen, and loaded into the sensor.…”

Section: Modification Of the Sensor Surface For Label-free And Fluorementioning

confidence: 99%

Long Range Surface Plasmons for Observation of Biomolecular Binding Events at Metallic Surfaces

2007

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A long range surface plasmon (LRSP) is an electromagnetic wave propagating along a thin metal film with an order of magnitude lower damping than conventional surface plasmon (SP) waves. Thus, the excitation of LRSP is associated with a narrower resonance and it provides larger enhancement of intensity of the electromagnetic field. In surface plasmon resonance (SPR) biosensors, these features allow a more precise observation of the binding of biomolecules in the proximity to the metal surface by using the (label-free) measurement of refractive index (RI) variations and by SP-enhanced fluorescence spectroscopy. In this contribution, we investigate LRSPs excited on a layer structure consisting of a fluoropolymer buffer layer, a thin gold film, and an aqueous sample. By implementing such structure in an SPR sensor, we achieved a 2.4-and 4.4-fold improvement of the resolution in the label-free and fluorescence-based detection, respectively, of the binding of biomolecules in the close proximity to the surface. Moreover, we demonstrate that the sensor resolution can be improved by a factor of 14 and 12 for the labelfree and fluorescence-based detection, respectively, if the biomolecular binding events occur within the whole evanescent field of LRSP.

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“…The research groups of Knoll [9,10] and Jung et al [11] have actively investigated the interaction of biotin and streptavidin monolayers on a variety of sensor formats. By exploring many different combinations of biotinylated thiols and using various spacer molecules, Knoll and co-workers have shown that an optimum monolayer of streptavidin can be generated by using a 1:9 mole ratio of biotin and hydroxy terminated thiols.…”

Section: Two-dimensional (2-d) Protein Microarray Surfacesmentioning

confidence: 99%

Application of self-assembly techniques in the design of biocompatible protein microarray surfaces

Schaeferling

Schiller

Paul³

et al. 2002

Electrophoresis

110

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Streptavidin arrays as supramolecular architectures in surface-plasmon optical sensor formats

Cited by 101 publications

References 31 publications

Molecular Recognition of Avidin on Biotin-Functionalized Gold Surfaces Detected by FT-IRRAS and Use of Metal Carbonyl Probes

Molecular Recognition of Avidin on Biotin-Functionalized Gold Surfaces Detected by FT-IRRAS and Use of Metal Carbonyl Probes

Long Range Surface Plasmons for Observation of Biomolecular Binding Events at Metallic Surfaces

Application of self-assembly techniques in the design of biocompatible protein microarray surfaces

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