Streptococcus mutans and Actinomyces naeslundii Interaction in Dual-Species Biofilm

Oliveira, Rosa Virginia Dutra de; Bonafé, Fernanda Salloume Sampaio; Spolidório, Denise Madalena Palomari; Koga‐Ito, Cristiane Yumi; Farias, Aline Leite de; Kirker, Kelly R.; James, Garth A.; Brighenti, Fernanda Lourenção

doi:10.3390/microorganisms8020194

Cited by 26 publications

(16 citation statements)

References 68 publications

(96 reference statements)

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“…Consistent with reported studies, we also found that A. johnsonii was enriched in the healthy controls. de Oliveira RVD reported that A. johnsonii is capable of increasing local pH by producing ammonia and alkali and can lead to a decrease in lactic acid production when interacting with S. mutans ( de Oliveira et al, 2020 ). Our results suggest that C. hominis was enriched in caries-free adolescents and that its connections with caries have not been reported.…”

Section: Discussionmentioning

confidence: 99%

Metagenomic Analysis of Dental Plaque on Pit and Fissure Sites With and Without Caries Among Adolescents

Pang¹,

Wang²,

Ye³

et al. 2021

Front. Cell. Infect. Microbiol.

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Caries is one of the most prevalent infectious diseases worldwide and is driven by the dysbiosis of dental biofilms adhering to tooth surfaces. The pits and fissured surfaces are the most susceptible sites of caries. However, information on the taxonomic composition and functional characteristics of the plaque microbiota in the pit and fissure sites is very limited. This study aimed to use metagenomic sequencing analyses to investigate the relationship between the plaque microbiome in the pit and fissure site and caries in adolescents. A total of 20 adolescents with active pit and fissure surface caries were involved as well as 20 age-matched, caries-free teenagers for control tests. Plaque samples were collected from the pit and fissure site and were subjected to metagenomic analyses, in which the microbial communities were investigated. Our results showed that the microbiota diversity was similar between those two groups. At the species level, the relative abundances of A. gerencseriae, P. acidifaciens, P. multisaccharivorax, S. oralis, S. mutans, and P. denticolens were higher in the caries-active group. N. elongata, C. hominis, and A. johnsonii were relatively more abundant in the caries-free groups. Functional analysis suggested that the metabolic pathway was the most abundant pathway, and the functional traits of the level 2 pathways included amino acid metabolism, metabolism of cofactors, and vitamins and carbohydrate metabolism. Our results also revealed that the caries group displayed several alterations in metabolic pathways, including enriched functions in carbohydrate digestion and absorption. This study suggested that in addition to the specific anatomical structures of the pit and fissured surfaces, the fundamental differences in the plaque microbiome may also be related to the susceptibility of pit and fissure caries.

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Section: Discussionmentioning

confidence: 99%

Metagenomic Analysis of Dental Plaque on Pit and Fissure Sites With and Without Caries Among Adolescents

Pang¹,

Wang²,

Ye³

et al. 2021

Front. Cell. Infect. Microbiol.

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“…It is, therefore, vital for the long-term sustainability of additively manufactured titanium devices to protect the surface against bacterial contamination. Among other species present in the oral cavity, Streptococcus mutans and Lactobacillus rhamnosus are especially important due to their ability to release lactic acid, decrease the pH, and survive in acidic environments, thereby becoming dominant corrosive microorganisms [29][30][31][32][33][34][35][36]. In addition to bacteria, it is worth mentioning Candida albicans, because of its symbiotic relations with S. mutans under biofilm formation.…”

Section: Introductionmentioning

confidence: 99%

“…In addition to bacteria, it is worth mentioning Candida albicans, because of its symbiotic relations with S. mutans under biofilm formation. This leads to increased glucan production, enhanced adherence, and increased biofilm complexity amplifying their harmful effect and hinders the effectiveness of antimicrobial substances [29,[33][34][35][36]. Therefore, these five species of microorganisms have been chosen in this study of biofilm formation on the 3D-printed raw fixation plates.…”

Section: Introductionmentioning

confidence: 99%

Susceptibility to biofilm formation on 3D-printed titanium fixation plates used in the mandible: a preliminary study

Pałka¹,

Mazurek-Popczyk

Arkusz

et al. 2020

Journal of Oral Microbiology

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Background: In the oral and maxillofacial surgery, fixation plates are commonly used for the stabilization of bone fragments. Additive manufacturing has enabled us to design and create personalized fixation devices that would ideally fit any given fracture. Aim: The aim of the present preliminary study was to assess the susceptibility of 3D-printed titanium fixation plates to biofilm formation. Methods: Plates were manufactured using selective laser melting (SLM) from Ti-6Al-4 V. Reference strains of Streptococcus mutans, Staphyloccocus epidermidis, Staphylococcus aureus, Lactobacillus rhamnosus , and Candida albicans , were tested to evaluate the material’s susceptibility to biofilm formation over 48 hours. Biofilm formations were quantified by a colorimetric method and colony-forming units (CFU) quantification. Scanning electron microscopy (SEM) visualized the structure of the biofilm. Results: Surface analysis revealed the average roughness of 102.75 nm and irregular topography of the tested plates. They were susceptible to biofilm formation by all tested strains. The average CFUs were as follows: S. mutans (11.91 x 10 7 ) > S.epidermidis (4.45 x 10 7 ) > S. aureus (2.3 x 10 7 ) > C.albicans (1.22 x 10 7 ) > L. rhamnosus (0.78 x 10 7 ). Conclusions: The present preliminary study showed that rough surfaces of additively manufactured titanium plates are susceptible to microbial adhesion. The research should be continued in order to compare additively manufactured plates with other commercially available osteotomy plates. Therefore, we suggest caution when using this type of material.

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“…Some studies have demonstrated that Actinomyces spp. play an essential role in the formation of dental biofilms and could contribute to the development of diseases such as caries and periodontitis ( de Oliveira et al., 2020 ). Dioguardi et al.…”

Section: Discussionmentioning

confidence: 99%

Distinctive microbiota distribution from healthy oral to post-treatment apical periodontitis

Zhang

Yun

Lin

et al. 2022

Front. Cell. Infect. Microbiol.

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Post-treatment apical periodontitis (PoAP) occurs when root canal treatment has not adequately eliminated bacterial invasion and infection. Yet little is known about the bacterial composition and changes related to the etiology and pathogenesis of PoAP. In this study, clinical samples classified as root apex (HARD) and periapical granulation tissues (SOFT) were separately collected from 10 patients with PoAP. The microbiota of each sample was characterized by 16S rRNA gene sequencing, and the obtained dataset was coanalyzed with 20 NCBI sequence read archive (SRA) datasets of healthy oral (HO) and primary apical periodontitis (PAP). We observed 2522 operational taxonomic units (OTUs) belonging to 29 phyla, and all samples shared 86.5% of the sequence reads. The OTUs affiliated with Bacteroidetes, Firmicutes, Proteobacteria, Fusobacteria, and Actinobacteria, were identified as core microbiota, which accounted for nearly 90% of 16S rRNA sequences in all samples. However, the principal coordinates analysis (PCoA) of the beta diversity demonstrated that the three periapical statuses have distinct microbial compositions. Compared with HO and PoAP, Actinomyces has a significantly increased abundance in PAP. The microbial diversities in PoAP were significantly lower than those in the HO and PAP (p<0.05). The relative abundance of most bacterial taxa was decreasing, except that Clostridia and Synergistia were increased. Furthermore, we explored the potential metabolic differences of the microbial communities by KEGG pathway prediction. We revealed that the microbiota of PoAP might have a more active metabolic capacity, including carbohydrate metabolism, energy metabolism, and enzyme cofactor/carrier biosynthesis (p<0.05). Our study revealed that invasion of opportunistic pathogens such as Clostridia and Synergistia might play a significant role in PoAP, thus guiding the further study of complex microbial-host interactions and the development of more effective diagnostic and therapeutic methods.

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Streptococcus mutans and Actinomyces naeslundii Interaction in Dual-Species Biofilm

Cited by 26 publications

References 68 publications

Metagenomic Analysis of Dental Plaque on Pit and Fissure Sites With and Without Caries Among Adolescents

Metagenomic Analysis of Dental Plaque on Pit and Fissure Sites With and Without Caries Among Adolescents

Susceptibility to biofilm formation on 3D-printed titanium fixation plates used in the mandible: a preliminary study

Distinctive microbiota distribution from healthy oral to post-treatment apical periodontitis

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