2006
DOI: 10.1099/mic.0.28747-0
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Streptococcus pneumoniae enolase is important for plasminogen binding despite low abundance of enolase protein on the bacterial cell surface

Abstract: Enolase represents one of the anchorless surface proteins of Streptococcus pneumoniae and has previously been identified as a plasminogen-binding protein, endowing this pathogen with host proteolytic activity. In this study the mAb 245,C-6 (IgG1) was produced in a BALB/c mouse after immunizing with a protein fraction from S. pneumoniae. The mAb reacted with recombinant pneumococcal enolase both under non-denaturing and denaturing conditions. The epitope for the mAb was mapped to residues 55 DKSRYGGLG 63 of pne… Show more

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Cited by 82 publications
(71 citation statements)
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“…However, it is well known from other bacterial pathogens that plasminogen receptors can be present at the cell surface at very low levels, as plasminogen is highly abundant in many body fluids (serum concentrations, ϳ1.6 to 2 mol/liter, roughly corresponding to 100 to 200 mg/liter) (2,12,35,39). The presence of pneumococcal enolase, which has been shown to act as the major plasminogen receptor of S. pneumoniae, at the surfaces of these bacteria has been studied in detail (29). Comparing these data, N. meningitidis plasminogen receptors are present at the meningococcal surface in at least the same quantity as pneumococcal enolase (29).…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…However, it is well known from other bacterial pathogens that plasminogen receptors can be present at the cell surface at very low levels, as plasminogen is highly abundant in many body fluids (serum concentrations, ϳ1.6 to 2 mol/liter, roughly corresponding to 100 to 200 mg/liter) (2,12,35,39). The presence of pneumococcal enolase, which has been shown to act as the major plasminogen receptor of S. pneumoniae, at the surfaces of these bacteria has been studied in detail (29). Comparing these data, N. meningitidis plasminogen receptors are present at the meningococcal surface in at least the same quantity as pneumococcal enolase (29).…”
Section: Discussionmentioning
confidence: 99%
“…The presence of pneumococcal enolase, which has been shown to act as the major plasminogen receptor of S. pneumoniae, at the surfaces of these bacteria has been studied in detail (29). Comparing these data, N. meningitidis plasminogen receptors are present at the meningococcal surface in at least the same quantity as pneumococcal enolase (29). The overall number of enolase, DnaK, and peroxiredoxin molecules was calculated in a quantitative ELISA to be approximately 10,000.…”
Section: Discussionmentioning
confidence: 99%
“…The identification of intracellular proteins on the cell surface is not unexpected, and numerous studies of S. aureus have reported a similar phenomenon (10,16,46,53,58). In some cases, alternative functions associated with the cell surface have been described for cytoplasmic proteins, including GAPDH (26,52), enolase (4,23), transcription elongation factors (1,24), and chaperone proteins GroES and DnaK (20,57). In addition, ribosomal proteins are commonly identified on the bacterial cell surface and can exhibit a high degree of immunogenicity for humans (25,48).…”
Section: Vol 79 2011mentioning
confidence: 91%
“…Likewise, when strain WU2 was analyzed, 5.68% of cells were positive, and 59.57% of the cells of its nonencapsulated mutant, JD908, were positive. PcsB at the plasma membrane is detectable by flow cytometric analysis, although the capsule and probably to a lesser degree other structures at the surface of the cell impede antibodies from interacting with PcsB, leading to higher detection on nonencapsulated than on encapsulated cells, as has also been seen with PspA and PspC (9).…”
Section: Real-time Pcr (Rtpcr)mentioning
confidence: 92%