Streptomyces actinomycinicus sp. nov., isolated from soil of a peat swamp forest

Tanasupawat, Somboon; Phongsopitanun, Wongsakorn; Suwanborirux, Khanit; Ohkuma, Moriya; Kudo, Takuji

doi:10.1099/ijsem.0.000716

Cited by 14 publications

(13 citation statements)

References 24 publications

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“…The major menaquinones were MK-9(H 8 ) (53.8 %), MK-9(H 6 ) (28.7 %), and MK-9(H 4 ) (13.4 %) while MK-10(H 4 ) (3.3 %), MK-10(H 2 ) (0.8 %) were also observed. The major menaquinone of CH5-8 T was similar to the major menaquinones found in S. actinomycinicus RCU-197 T [48]. Diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), hydroxyphosphatidylethanolamine (OH-PE), phosphatidylglycerol (PG), phosphatidylinositol (PI), phosphatidylinositol mannoside (PIM), four unidentified phospholipids (PLs) and two unidentified lipids (Ls) were observed as the polar lipids in cells (Fig.…”

Section: Resultssupporting

confidence: 66%

Streptomyces musisoli sp. nov., an actinomycete isolated from soil

Duangupama

Intaraudom

Pittayakhajonwut

et al. 2021

International Journal of Systematic and Evolutionary Microbiology

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An actinobacterium, strain CH5-8T, which formed spiral chains of spore arising from the aerial mycelium, was isolated from rhizosphere soil of Musa spp. The organism exhibited vivid greenish yellow substrate mycelium and easily produced the medium grey aerial spore mass on ISP2 medium. The typical chemotaxonomic properties of members of the genus Streptomyces were observed for strain CH5-8T, e.g. ll-diaminopimelic acid in cell peptidoglycan, MK-9(H8), MK-9(H6), and MK-9(H4) as major menaquinones and anteiso-C15 : 0, iso-C16 : 0, and anteiso-C17 : 0 as major fatty acids. Diphosphatidylglycerol, phosphatidylethanolamine, hydroxyphosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside were detected in the cells. A combination of morphological and chemotaxonomic data supported the assignment to the genus Streptomyces . The analysis result obtained for the 16S rRNA gene sequence confirmed the taxonomic affiliation at the genus level of this strain. The novel strain CH5-8T showed the highest 16S rRNA gene sequence values to Streptomyces echinatus NBRC 12763T (98.9 %), followed by Streptomyces actinomycinicus RCU-197T (98.9 %). The average nucleotide identity by blast (ANIb) and digital DNA–DNA hybridization values between CH5-8T and its closest relatives, S. echinatus CECT 3313T and S. actinomycinicus RCU-197T, were ≤91.6 % and ≤47.4 %, respectively. The digital DNA G+C content of genomic DNA was 72.1 mol%. On the basis of these phenotypic and genotypic data, strain CH5-8T represents a novel species, for which the name Streptomyces musisoli sp. nov. is proposed. The type strain is CH5-8T (=TBRC 9950T=NBRC 113997T).

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Section: Resultssupporting

confidence: 66%

Streptomyces musisoli sp. nov., an actinomycete isolated from soil

Duangupama

Intaraudom

Pittayakhajonwut

et al. 2021

International Journal of Systematic and Evolutionary Microbiology

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“…Actinomycin D is a secondary metabolite produced by many streptomycetes, including S. actinomycinicus RCU-197 T , the closest related strain of PJ85 ( Figure 1 ). It has been reported that the sole antibacterial agent produced by S. actinomycinicus RCU-197 T is actinomycin D [ 30 ]. However, an unidentified bioactive agent, compound 2, was also detected from the PJ85 crude extract ( Figure 4 ).…”

Section: Resultsmentioning

confidence: 99%

Isolation and Identification of Bioactive Compounds from Streptomyces actinomycinicus PJ85 and Their In Vitro Antimicrobial Activities against Methicillin-Resistant Staphylococcus aureus

Chanthasena

Hua

Rosyidah³

et al. 2022

Antibiotics

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Antibiotic-resistant strains are a global health-threatening problem. Drug-resistant microbes have compromised the control of infectious diseases. Therefore, the search for a novel class of antibiotic drugs is necessary. Streptomycetes have been described as the richest source of bioactive compounds, including antibiotics. This study was aimed to characterize the antibacterial compounds of Streptomyces sp. PJ85 isolated from dry dipterocarp forest soil in Northeast Thailand. The 16S rRNA gene sequence and phylogenetic analysis showed that PJ85 possessed a high similarity to Streptomyces actinomycinicus RCU-197T of 98.90%. The PJ85 strain was shown to produce antibacterial compounds that were active against Gram-positive bacteria including methicillin-resistant Staphylococcus aureus (MRSA). The active compounds of PJ85 were extracted and purified using silica gel column chromatography. Two active antibacterial compounds, compound 1 and compound PJ85_F39, were purified and characterized with spectroscopy, including liquid chromatography and mass spectrometry (LC–MS). Compound 1 was identified as actinomycin D, and compound PJ85_F39 was identified as dihomo-γ-linolenic acid (DGLA). To the best of our knowledge, this is the first report of the purification and characterization of the antibacterial compounds of S. actinomycinicus.

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“…Carbon-nitrogen sources can also be added to the isolation media; casein [55,56], peptone [8,57], malt extract [58] and yeast extract [53,58,59]. The isolation media can be supplemented by K 2 Cr 2 O 7 [54,[60][61][62][63][64], nalidixic acid, nystatin, cycloheximide, rifampicin, and tetracycline [37,38,40,49,51,53,55,56,58,59,[65][66][67][68][69] to inhibit the growth of unwanted bacteria and fungi (See Tables 1 and S1).…”

Section: Extreme Environmentsmentioning

confidence: 99%

“…This is advantageous when performing pretreatments to selectively isolate Streptomyces spp. Some of the commonly used pretreatments for the isolation of Streptomyces from an extreme terrestrial environment include distilled water with added NaCl [38,52,71,72], air-drying [53,64,68] pretreatment by ultrasound [56,69] and agitation on a rotary stirrer [37,40,54,57,66,69]. Dry heat treatment and wet heat treatment are the most frequently used pretreatments.…”

Section: Extreme Environmentsmentioning

confidence: 99%

Streptomyces: Still the Biggest Producer of New Natural Secondary Metabolites, a Current Perspective

Donald

Pipite

Subramani

et al. 2022

Microbiology Research

101

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There is a real consensus that new antibiotics are urgently needed and are the best chance for combating antibiotic resistance. The phylum Actinobacteria is one of the main producers of new antibiotics, with a recent paradigm shift whereby rare actinomycetes have been increasingly targeted as a source of new secondary metabolites for the discovery of new antibiotics. However, this review shows that the genus Streptomyces is still the largest current producer of new and innovative secondary metabolites. Between January 2015 and December 2020, a significantly high number of novel Streptomyces spp. have been isolated from different environments, including extreme environments, symbionts, terrestrial soils, sediments and also from marine environments, mainly from marine invertebrates and marine sediments. This review highlights 135 new species of Streptomyces during this 6-year period with 108 new species of Streptomyces from the terrestrial environment and 27 new species from marine sources. A brief summary of the different pre-treatment methods used for the successful isolation of some of the new species of Streptomyces is also discussed, as well as the biological activities of the isolated secondary metabolites. A total of 279 new secondary metabolites have been recorded from 121 species of Streptomyces which exhibit diverse biological activity. The greatest number of new secondary metabolites originated from the terrestrial-sourced Streptomyces spp.

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Streptomyces actinomycinicus sp. nov., isolated from soil of a peat swamp forest

Cited by 14 publications

References 24 publications

Streptomyces musisoli sp. nov., an actinomycete isolated from soil

Streptomyces musisoli sp. nov., an actinomycete isolated from soil

Isolation and Identification of Bioactive Compounds from Streptomyces actinomycinicus PJ85 and Their In Vitro Antimicrobial Activities against Methicillin-Resistant Staphylococcus aureus

Streptomyces: Still the Biggest Producer of New Natural Secondary Metabolites, a Current Perspective

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