Streptomyces cahuitamycinicus sp. nov., isolated from desert soil and reclassification of Streptomyces galilaeus as a later heterotypic synonym of Streptomyces bobili

Saygin, Hayrettin; Ay, Hilal; Güven, Kıymet; Çetin, Demet; Şahi̇n, Nevzat

doi:10.1099/ijsem.0.004103

Cited by 29 publications

(17 citation statements)

References 68 publications

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“…Meanwhile, ATCC 15439 could be classified as a distinct species from other S. venezuelae species according to the ANIm analysis, and its genome encodes many strain-specific smBGCs. Several reclassifications of Streptomyces have been reported for species-level using a whole-genome comparison approach [ 58 , 59 ].…”

Section: Discussionmentioning

confidence: 99%

Comparative Genomics Determines Strain-Dependent Secondary Metabolite Production in Streptomyces venezuelae Strains

et al. 2020

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Streptomyces venezuelae is well known to produce various secondary metabolites, including chloramphenicol, jadomycin, and pikromycin. Although many strains have been classified as S. venezuelae species, only a limited number of strains have been explored extensively for their genomic contents. Moreover, genomic differences and diversity in secondary metabolite production between the strains have never been compared. Here, we report complete genome sequences of three S. venezuelae strains (ATCC 10712, ATCC 10595, and ATCC 21113) harboring chloramphenicol and jadomycin biosynthetic gene clusters (BGC). With these high-quality genome sequences, we revealed that the three strains share more than 85% of total genes and most of the secondary metabolite biosynthetic gene clusters (smBGC). Despite such conservation, the strains produced different amounts of chloramphenicol and jadomycin, indicating differential regulation of secondary metabolite production at the strain level. Interestingly, antagonistic production of chloramphenicol and jadomycin was observed in these strains. Through comparison of the chloramphenicol and jadomycin BGCs among the three strains, we found sequence variations in many genes, the non-coding RNA coding regions, and binding sites of regulators, which affect the production of the secondary metabolites. We anticipate that these genome sequences of closely related strains would serve as useful resources for understanding the complex secondary metabolism and for designing an optimal production process using Streptomyces strains.

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Section: Discussionmentioning

confidence: 99%

Comparative Genomics Determines Strain-Dependent Secondary Metabolite Production in Streptomyces venezuelae Strains

et al. 2020

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“…Gram-staining was tested as described by Magee et al [42]. Cultural characteristics of strain HC44 T were observed following growth on Czapek's agar [43], nutrient agar [44], modified Bennett's agar [45], tryptic soy agar (Difco) and ISP 2-7 agar [34] (4,10,20,28,37,45,50 and 55 °C), NaCl (w/v) concentrations (0-10 % NaCl at intervals of 1.0 %) and pH values (from pH 4.0 to 12.0 at intervals of 1.0 pH unit). KH 2 PO 4 -HCl, KH 2 PO 4 -K 2 HPO 4 and K 2 HPO 4 -NaOH buffer systems were used to maintain the pH values of the media.…”

Section: Cultural Morphological and Physiological Propertiesmentioning

confidence: 99%

“…Members of the genus are known to produce biologically active compounds, such as antibiotics, enzymes and anticancer metabolites [5]. In recent years, numerous studies on the subject of reclassifications and emendations of the members of the genus based on multilocus sequence analysis (MLSA) and whole genome analysis have been carried out and, as a result, there has been serious decrease in the number of species [6][7][8][9][10]. At the time of writing, the genus comprises 664 species with a validly published and correct name (https:// lpsn.…”

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confidence: 99%

Streptomyces scabichelini sp. nov., isolated from soil

Gencbay

Saygin

Güven

et al. 2021

International Journal of Systematic and Evolutionary Microbiology

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A novel actinomycete, designated strain HC44T, was isolated from a soil sample collected from Hacibektaş, Turkey, and characterized using a polyphasic approach. The strain had morphological characteristics and chemotaxonomic properties identical to those of members of the genus Streptomyces . Phylogenetic analyses based on 16S rRNA gene sequence comparisons revealed that HC44T clustered with members of the genus Streptomyces and the highest 16S rRNA gene sequence similarity values were obtained with Streptomyces vastus NBRC 13094T (97.6 %) and Streptomyces kalpinensis TRM 46509T (96.9 %). Multi-locus sequence analysis (MLSA) based on five housekeeping genes (atpD, gyrB, recA, rpoB and trpB) showed that the MLSA evolutionary distance value was 0.043 between strain HC44T and S. vastus NBRC 13094T. Whole-cell hydrolysates contained ll-diaminopimelic acid, glucose, mannose and ribose. The predominant menaquinones were MK-9(H6) and MK-9(H8). The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside. The DNA G+C content of the draft genome sequence, consisting of 11.2 Mbp, was 69.8 mol%. On the basis of polyphasic taxonomic evidence, strain HC44T represents a novel species of the genus Streptomyces , for which the name Streptomyces scabichelini sp. nov. is proposed. The type strain is HC44T (=DSM 106874T=KCTC 39872T).

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“…Members of the genus Streptomyces are widely described among Actinobacteria as antibiotic producers [7]. Therefore, many studies have aimed to reveal the isolation, identification and characterization of members of the genus Streptomyces [8–14].…”

Section: Full-textmentioning

confidence: 99%

Streptomyces coryli sp. nov., isolated from hazelnut orchard soil

Saygin

Veyisoglu

Tatar

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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A novel actinobacteria, isolate A7024T, was isolated from commercial hazelnut orchard soil sample which was collected at Duzce, West Black Sea region, Turkey. A polyphasic taxonomic study was carried out to determine the status of this isolate. The phylogenetic tree reconstructed using the neighbour-joining algorithm based on 16S rRNA gene sequences indicated that isolate A7024T was positioned within the members of the genus Streptomyces with the highest sequence similarity (97.7 %) to Streptomyces cadmiisoli ZFG47T. The organism formed an extensively branched substrate and aerial hyphae which generated irregular rod-shaped spores with smooth-surfaces. The cell wall of strain A7024T contained ll-diaminopimelic. Glucose, mannose and ribose were detected as whole-cell sugars. Its polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, three unidentified phospholipids and three unidentified glycolipids. Major menaquinones were MK-9(H6) and MK-9(H4). The major cellular fatty acids were iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0. Strain A7024T had a genome size of 9.0 Mb with a genome G+C content of 71.5 mol%. The low level of 16S rRNA gene similarity, 19.3 ± 2.3% digital DNA–DNA hybridization and 76.94 % average nucleotide identity values, as well as some different phenotypic characteristics allowed the strain to be distinguished from the closely related type strains. Therefore, it is concluded that strain A7024T represents a novel species of the genus of Streptomyces , for which the name Streptomyces coryli sp. nov. is proposed. The type strain is A7024T (=DSM 42066T=KCTC 29102T=NRRL B-24888T).

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Streptomyces cahuitamycinicus sp. nov., isolated from desert soil and reclassification of Streptomyces galilaeus as a later heterotypic synonym of Streptomyces bobili

Cited by 29 publications

References 68 publications

Comparative Genomics Determines Strain-Dependent Secondary Metabolite Production in Streptomyces venezuelae Strains

Comparative Genomics Determines Strain-Dependent Secondary Metabolite Production in Streptomyces venezuelae Strains

Streptomyces scabichelini sp. nov., isolated from soil

Streptomyces coryli sp. nov., isolated from hazelnut orchard soil

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