Streptomyces capparidis sp. nov., a novel endophytic actinobacterium isolated from fruits of Capparis spinosa L.

Abstract

A novel endophytic actinobacterial strain, designated EGI 6500195T, was isolated from fruits of Capparis spinosa. Growth occurred at 10-45 °C (optimum 30 °C), at pH 6-8 (optimum pH 7) and in the presence of 0-1 % (w/v) NaCl. Strain EGI 6500195T shared highest 16S rRNA gene sequence similarity (97.74 %) with Streptomyces vitaminophilus DSM 41686T and less than 97 % sequence similarity with other members of the genus Streptomyces. The diagnostic amino acid in the peptidoglycan was ll-diaminopimelic acid. Whole-cell hydrolysates contained glucose, ribose, fructose and mannose. The predominant menaquinones were MK-9(H6) and MK-9(H8). The polar lipid profile of strain EGI 6500195T included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylinositol, phosphatidylcholine, three unknown phospholipids, an unknown aminophospholipid and an unknown aminolipid. The cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0, iso-C15 : 0, iso-C16 : 0, anteiso-C17 : 1ω9c, summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B) and iso-C17 : 1ω9c. The DNA G+C content of strain EGI 6500195T was 74.1 mol%. The level of DNA-DNA relatedness between strain EGI 6500195T and Streptomyces. vitaminophilus DSM 41686T was 14.1±3.5 %. On the basis of the phenotypic, phylogenetic, chemotaxonomic and DNA-DNA hybridization data, strain EGI 6500195T represents a novel species of the genus Streptomyces, for which the name Streptomyces capparidis sp. nov. is proposed. The type strain is EGI 6500195T (=DSM 42145T=JCM 30089T).

Streptomyces nigra sp. nov. Is a Novel Actinobacterium Isolated From Mangrove Soil and Exerts a Potent Antitumor Activity in Vitro

“…The fatty acid and polar lipid profiles of strain 452 T were similar to those of the reference strains (Supplementary Table S3 ), despite some differences in their proportions and minor constituents, respectively. Chemotaxonomic features of strain 452 T were also similar to the features of other species from the genus Streptomyces ( Lee and Whang, 2016 ; Ser et al, 2016a ; Law et al, 2017 ; Röttig et al, 2017 ; Wang et al, 2017 ).…”

“…Strain 452 T was incubated at 28°C for 5 days on MA medium, and cell morphology was examined and observed by transmission electron microscopy (80 kV, JEM-1230; Jeol) after uranyl acetate (0.5%, w/v) staining, scanning electron microscope (3.0 kV, SU8010, Hitachi) fixation by osmium tetroxide vapor (4%, w/v), and optical microscopy (BX40; Olympus) after gram staining. The culture characteristics were determined following growth on ISP 2, ISP 3, ISP 4, ISP 5, ISP 6, and ISP 7 agars ( Shirling and Gottlieb, 1966 ); Streptomyces agar ( Atlas, 2010 ), starch casein nitrate agar ( Ser et al, 2016b ), nutrient agar ( Odds, 1981 ), potato-glucose agar ( Wang et al, 2017 ), tryptone soya agar ( Williams et al, 1983 ), and MA 2216 (Difco) for 14 days at 28°C. The colors of substrate, aerial mycelium and any soluble pigments produced were determined by comparison with chips from the ISCC-NBS color charts ( Kelly and Judd, 1964 ).…”

Streptomyces: Still the Biggest Producer of New Natural Secondary Metabolites, a Current Perspective

“…Different parts of plants are sampled for the isolation of Streptomycetes, including tree bark [79], leaf litter [80], bulbil [81], roots [82][83][84], stem [85][86][87], fruits [88], seeds [89] and phylloplane [90] (Table 2). Mosses have also been recorded as a source of novel Streptomyces spp.…”

Streptomyces cahuitamycinicus sp. nov., isolated from desert soil and reclassification of Streptomyces galilaeus as a later heterotypic synonym of Streptomyces bobili

“…This genus is responsible for a significant portion of all isolated and identified natural compounds such as antibiotics [10, 11]. For this reason, the genus Streptomyces has become the main focus of isolation and investigation studies of the novel species from different habitats such as deserts [12–15], bats [16], fruits [17], insects [18, 19], lake sediments [20, 21], manganese-contaminated soil [22], mangrove sediments [23, 24], mangrove soil [25], marine sponges [26, 27], marine sediments [28, 29], rhizospheres [30, 31], zinc and copper mine [32], and plant tissues [33, 34] and soil [35, 36]. In this study, it was aimed to isolate novel actinobacteria from soil samples collected from the Karakum Desert.…”