Streptomyces cynarae sp. nov., a novel actinomycete isolated from the leaves of Cynara scolymus L

Deng, Aihua; Luo, Yijia; Wu, Can; Chen, Dan; Mo, Ping; Li, Baiyuan

doi:10.1007/s10482-023-01885-2

Cited by 1 publication

(2 citation statements)

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“…The samples were kept in an ice box until the isolation attempt. The leaf surfaces were sterilized according to the method described by Deng et al [15]. The treated leaves were dried at 60 °C for 3 days.…”

Section: Methodsmentioning

confidence: 99%

“…After 21 days at 28 °C, the colours were determined by comparison with the Color Standards and Color Nomenclature [20]. The inoculated plates with strain HUAS 3-15 T and K. aureofaciens JCM 4008 T were cultured at different temperatures (4,10,15,20,25,30,35,37,40,45,50 and 55 °C) for 14 days. The ISP 2 liquid medium was adjusted by the following buffer systems to pH 4.0-5.0 with 0.1 M citric acid, to pH 6.0-8.0 with 0.1 M KH 2 PO 4 /0.1 M NaOH, to pH 9.0-10.0 with 0.1 M NaHCO 3 /0.1 M Na 2 CO 3 , to pH 11.0 with 0.05 M Na 2 HPO 4 /0.1 M NaOH, to pH 12.0-13.0 with 0.2 M KCl/0.2 M NaOH.…”

Section: Phenotypic Tests Morphological and Cultural Propertiesmentioning

confidence: 99%

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Kitasatospora cathayae sp. nov., a novel endophytic actinomycete isolated from the leaves of Cathaya argyrophylla

Zheng,

Mo,

et al. 2024

International Journal of Systematic and Evolutionary Microbiology

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Strain HUAS 3-15T was isolated from the leaves of Cathaya argyrophylla collected from Chenzhou, Hunan Province, PR China. The main fatty acids (>5.0 %) of the strain were anteiso-C15 : 0, C16 : 0, C18 : 1 ω9c, iso-C16 : 0, summed feature 5 (C18 : 2 ω6,9c/C18 : 0 ante), iso-C15 : 0 and anteiso-C17 : 0. MK-9(H6), MK-9(H8) and MK-9(H4) were detected as respiratory quinones. The diagnostic cell-wall diamino acid was meso-diaminopimelic acid. Galactose, glucose and ribose were also present in the cell wall. The major polar lipids consisted of diphosphatidylglycerol, phosphatidyl ethanolamine, phosphatidylinositol mannosides and unidentified phospholipids. The DNA G+C content of the genome sequence, consisting of 8 860 963 bp, is 72.4 mol%. blast analysis based on 16S rRNA gene sequences revealed that the strain belongs to the genus Kitasatospora, with 99.37, 99.03, 98.95, 98.68 and 98.67 % sequence similarity to Kitasatospora aureofaciens ATCC 10762T, Kitasatospora viridis DSM 44826T, Kitasatospora xanthocidica NBRC 13469T, Kitasatospora aburaviensis NRRL B-2218T and Kitasatospora kifunensis IFO 15206T, respectively. Phylogenetic trees based on 16S rRNA gene and whole-genome sequences demonstrated that strain HUAS 3-15T formed a well‐supported cluster with K. aureofaciens ATCC 10762T. Further genomic characterization through average nucleotide identity (ANIb/m) and digital DNA–DNA hybridization analysis between strain HUAS 3-15T and K. aureofaciens ATCC 10762T showed values of 90.62/92.55 % and 45.3 %, respectively, lower than the 95–96 % ANI threshold and 70.0 % cutoff used as guideline values for species delineation in bacteria. Furthermore, the differences between the strain and its phylogenomic neighbour in terms of physiological (e.g. sole carbon source growth) and chemotaxonomic (e.g. cellular fatty composition) characteristics further supported this conclusion. Consequently, we concluded that strain HUAS 3-15T represents a novel species of the genus Kitasatospora, for which the name Kitasatospora cathayae sp. nov. is proposed. The type strain is HUAS 3-15T (=MCCC 1K08542T=JCM 36274T).

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Section: Methodsmentioning

confidence: 99%