Streptomyces sp. RP1A-12 mediated control of peanut stem rot caused by Sclerotium rolfsii

Aim The aim of this study was to screen potential plant growth promoting rhizobacterial (PGPR) actinobacterial isolate with effective inhibition against anthracnose causing fungal pathogen Colletotrichum capsici. Methods and Results In this study, actinobacterias were isolated from rhizosphere soil using dilution plate method and tested for antagonistic potential against pathogenic fungi C. capsici. In primary and secondary screening tests, the actinobacterial isolate BS‐26 displayed high antagonistic activity against the fungal pathogen. Isolate BS‐26 was identified as Streptomyces violaceoruber based on 16S rDNA sequencing. Furthermore, indole acetic acid production, phosphate solubilization and ammonia production have been confirmed in the S. violaceoruber that suggest their potential to be used as PGPR bacteria. A green house experiment showed that application of S. violaceoruber fermentation broth reduced the incidence of the chilli anthracnose and promoted the growth of chilli seedlings with a significant increase in germination %, total plant height, fresh weight and chlorophyll content when compared to controls. Conclusion Streptomyces violaceoruber can be applied as a biofertilizer and biocontrol agent for growing chillies against the attack of fungal pathogen C. capsici. Significance of Impact of the Study The damage caused by anthracnose disease is an issue of concern, affecting negatively the economy involved in chilli cultivation. As chemical methods of control have serious disadvantages, biocontrol approach using beneficial (PGPR) micro‐organisms shall be a better alternative to control crop diseases.

show abstract

“…VI was calculated based on the following formula and expressed as whole number (Jacob et al . )

\begin{matrix} Vigour index (VI) & = Germination (%) \\ \times Mean total length of seedling in cm \end{matrix}

…”

Section: Methodsmentioning

confidence: 99%

“…Percent seed germination (G%) was calculated using the formula G% ¼ ðNumber of germinated seeds/Total number of seedssownÞ Â 100 VI was calculated based on the following formula and expressed as whole number (Jacob et al 2018) Vigour index (VI) ¼ Germinationð%Þ Â Mean total length of seedling in cm…”

Section: Measurement Of Plant Growth Parametersmentioning

confidence: 99%

Plant growth promotion and chilli anthracnose disease suppression ability of rhizosphere soil actinobacteria

Thilagam

Hemalatha

2019

J Appl Microbiol

View full text Add to dashboard Cite

show abstract

“…(Suwan et al 2012), Rhizoctonia solani (Goudjal et al 2014), and Sclerotium sp. (Jacob et al 2018). The search for new antifungal compounds produced by the gram-positive bacteria with high DNA G+C contents also due to Actinomycetes has a broad ecological distribution covering from terrestrial, freshwater to marine environments.…”

Section: Introductionmentioning

confidence: 99%

Growth inhibition of Fusarium solani and F. oxysporum by Streptomyces sasae TG01, and its ability to solubilize insoluble phosphate

et al. 2020

View full text Add to dashboard Cite

Abstract. Sudiana A, Putri A, Napitupulu TP, Purnaningsih I, Idris, Kanti A. 2020. Growth inhibition of Fusarium solani and F. oxysporum by Streptomyces sasae TG01, and its ability to solubilize insoluble phosphate. Biodiversitas 21: 429-435. Actinomycetes have been widely explored for new antibiotic production, but not many studies explore its abilities to inhibit the growth of phytopathogenic fungi and solubilize insoluble phosphate hence stimulate the growth of plants. We isolated Actinomycetes from the soil. Based on morphology, physiology, and 16S rDNA analyses, the isolate is closely related to Streptomyces sasae. The strain was able to inhibit the growth of phytopathogenic fungi Fusarium solani, and Fusarium oxysporum. S. sasae produced secondary metabolites 2-methyl-1,3-dioxolane as the major constituent. The strain assimilated variable carbon sources include L-arabinose, D-fructose, D-glucose, D-mannitol, Lactose, raffinose, L-rhamnose, and sucrose. The strain grew at pH 6.0 to 8.0, and at salinity (1-3%). Their growth was affected by the salinity level. The strain solubilized Ca-P at 1-3% salinity, but their ability to solubilize phosphate was influenced by salinity. The strain was also able to solubilize rock phosphate. Their ability to solubilize less soluble phosphate and inhibit the growth of F. solani and F. oxysporum may imply that this strain is potential for biocontrol agents. The 16S rRNA gene was submitted to DDBJ with the entry number 5df623c1a3c8820021322a36.TG01, and the accession number is LC514451.

show abstract

“…S. rolfsii is a destructive soil-borne fungal pathogen with a wide host range, which affects crop productivity and causes large scale economic losses. Among its many host genera, peanut sustains high yield losses [ 19 ]. Prothioconazole is a highly effective broad-spectrum triazole fungicide, which was developed by Bayer Crop Science and listed in 2004.…”

Section: Introductionmentioning

confidence: 99%

Effective and Sustained Control of Soil-Borne Plant Diseases by Biodegradable Polyhydroxybutyrate Mulch Films Embedded with Fungicide of Prothioconazole

Chen

Cao²,

Cao

et al. 2021

Molecules

View full text Add to dashboard Cite

Soil-borne diseases and plant rhizosphere nematode have caused many crop yield losses. Increased environmental awareness is leading to more restrictions on the use of certain fumigants and root irrigation methods due to their impact on human health and soil system. Therefore, it is necessary to find alternative treatments to maintain crop economic yields and environmental sustainability. In the present work, biodegradable antifungal mulches were prepared by blending poly(3-hydroxybutyrate-co-4-hydroxybutyrate) (PHB) with fungicide of prothioconazole (PRO), which were used for effective and sustained control of soil-borne plant diseases. To reveal the application prospect of the PHB/PRO composite films in the management of soilborne plant diseases, some physical and biological properties were evaluated. The proper mulch film of PHB/PRO was assessed based on its mechanical and optical properties, while water solubility and the film micromorphology was further characterized. The release patterns of composite films under different pH levels were investigated. Moreover, the in vitro antifungal bioassay and pot experiment showed satisfactory bioactivity of the PHB/PRO films against Sclerotium rolfsii Sacc., a soil-borne disease in peanut fields. This study demonstrated that the biodegradable mulch films containing PRO fungicide are capable of inhibiting soil-borne plant pathogenic fungi effectively, and this facile but powerful strategy may find wide applicability in sustainable plant and horticulture protection.

show abstract

Streptomyces sp. RP1A-12 mediated control of peanut stem rot caused by Sclerotium rolfsii

Cited by 29 publications

References 45 publications

Plant growth promotion and chilli anthracnose disease suppression ability of rhizosphere soil actinobacteria

Plant growth promotion and chilli anthracnose disease suppression ability of rhizosphere soil actinobacteria

Growth inhibition of Fusarium solani and F. oxysporum by Streptomyces sasae TG01, and its ability to solubilize insoluble phosphate

Effective and Sustained Control of Soil-Borne Plant Diseases by Biodegradable Polyhydroxybutyrate Mulch Films Embedded with Fungicide of Prothioconazole

Contact Info

Product

Resources

About