Stress and steroid interaction modulates expression of estrogen receptor alpha in the brain, pituitary, and testes of immature<i>Gallus gallus domesticus</i>

Baghel, Kalpana

doi:10.1080/10253890.2021.1965119

Cited by 9 publications

(8 citation statements)

References 47 publications

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“…After completion of experimental duration, quails were sacrificed; testes were excised, weighed, and kept in fixative. Sexual maturity of quails was calculated by gonado‐somatic‐index (GSI) with the formula: GSI = (weight of testes/body weight) ×100 11 . Five μm thick sections were cut with rotary microtome (Leica RM2325).…”

Section: Methodsmentioning

confidence: 99%

“…Expression of ERα has been observed in all testicular cells as spermatocytes, spermatids, Leydig's cells, Sertoli cells as well as mature spermatozoa. [9][10][11] ER-β is also reported in all germ cells. Also, lack of estrogen receptors in male reproductive system can lead to structural and functional interruption.…”

Section: Introductionmentioning

confidence: 93%

“…Estrogen signaling is important for regulating male reproductive function. Expression of ERα has been observed in all testicular cells as spermatocytes, spermatids, Leydig's cells, Sertoli cells as well as mature spermatozoa 9–11 . ER‐β is also reported in all germ cells.…”

Section: Introductionmentioning

confidence: 98%

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Dietary restriction‐induced alterations on estrogen receptor alpha expression in regulating fertility in male Coturnix coturnix japonica: Relevance of Withania somnifera in modulation of inflammation and oxidative stress in testis

Baghel,

Azam,

Srivastava

2024

American J Rep Immunol

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ProblemReproductive performance of animals gets affected by nutritional restrictions which act as potential stressors leading to hormonal imbalance and testicular inflammation, the major causes of infertility. Withania somnifera (WS), well‐known traditional medicinal plant, has been used as antistress and infertility treatment. Therefore, the present study looks into the ameliorative effects of WS on the reproductive and immune system of male Coturnix coturnix japonica in stressed conditions like water and food restriction focussing on the modulation in estrogen receptor alpha (ERα).Method of studyBiochemical estimations for oxidative stress, histological alterations, immuno‐fluorescent localization of ERα, interleukin (IL)‐1β, IL‐4, and interferon gamma (IFN‐γ) in testicular cells were performed.ResultsNutritional restriction declines endogenous estradiol, ERα in testicular cells while it elevates corticosterone leading to oxidative stress in testis thereby reducing fertility by decrease in sperm. Results indicate significant reversal in all the parameters after the administration of WS by improving testicular cell morphology, increased superoxide and catalase activity thus reducing oxidative stress. WS increases spermatogenesis and enhances expression of ERα in testicular cells in quail. Further, WS increases IL‐4, decreases IL‐1β and IFN‐γ expression in testis, thereby improving immune profile contrary to stressed conditions.ConclusionWS stimulates HPG‐axis even after stress resulting in increased endogenous estradiol which stimulates the expression of ERα in testis; increases sperm count and immunity thereby improving the reproductive performance. WS may be the best therapy against nutritional‐restriction stress induced reproductive toxicity by reducing oxidative stress mediated inflammatory response via increased testicular expression of ERα in quail.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 93%

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Dietary restriction‐induced alterations on estrogen receptor alpha expression in regulating fertility in male Coturnix coturnix japonica: Relevance of Withania somnifera in modulation of inflammation and oxidative stress in testis

Baghel,

Azam,

Srivastava

2024

American J Rep Immunol

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“…Six histological sections were randomly selected from each group and were used for measurement of the diameter of seminiferous tubules by using an occulometer and micrometre (ERMA Japan). The testicular volume was also calculated using Bissonett's formula: 4/3πab 2 (where a = half of the long axis and b = half of the short axis) as de ned earlier 40,41 . Testicular density was measured by the following formula: testes weight/volume and expressed (g/cm − 3 ) 42 .…”

Section: Histology Of Pancreas Brain and Testesmentioning

confidence: 99%

“…The sections were counterstained with 4′, 6-diamidino-2phenylindole (DAPI) for 6 min at 37°C, then mounted by glycerine-based mounting media and visualized with a uorescent microscope (EVOSM5000 -Invitrogen Thermo sher scienti c). Negative control slides were processed without adding primary antibodies 41 .…”

Section: Immuno-uorescent Localization Of Gnrh-i Erα and Caspase-3mentioning

confidence: 99%

Withaferin-A attenuates T2DM-induced male reproductive dysfunction mediated by ERα in brain and testes of Swiss albino mice

Baghel

Azam

Srivast

et al. 2023

Preprint

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Type 2 diabetes mellitus (T2DM) is a chronic metabolic disease, characterized by persistence hyperglycemia resulting from diminished response to insulin secretion or resistance. The present study evaluated the ameliorative effects of Withaferin-A on T2DM-induced reproductive dysfunction in mice. For the same, mice were intraperitoneally injected with Streptozotocin (STZ), (40 mg/kg/day) for 5 consecutive days to induce T2DM. Mice were then treated with WA (8 mg/kg/day) both in normal and diabetic conditions (STZ+WA). Next, blood glucose levels, OGTT, IPTT, oxidative stress and reproductive parameters were estimated. For reproductive performance, immunofluorescent localization of GnRH-I and ERα in POA and PVN region of hypothalamus and ERα in testes was performed. STZ-induced diabetes triggered reproductive dysfunctions as mediated by low ir-GnRH-I and ERα in the brain and ERα in the testes along with declined testosterone and estradiol levels. Interestingly, treatment with WA significantly reduced the blood glucose levels and enhanced glucose clearance including oxidative stress in the brain, pancreas and testes as indicated by low levels of H2O2 and MDA in STZ+WA. This study report for the first time that WA can efficiently combat T2DM induced reproductive dysfunction via enhancing endogenous testosterone, estrogen and increased ir-GnRH-I and ERα in the brain and ERα in the testes of T2DM mice.

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Ashwagandha Diminishes Hippocampal Apoptosis Induced by Microwave Radiation by Acetylcholinesterase Dependent Neuro-Inflammatory Pathway in Male Coturnix coturnix Japonica

Gupta,

Srivastava

2024

Neurochem Res

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Stress and steroid interaction modulates expression of estrogen receptor alpha in the brain, pituitary, and testes of immatureGallus gallus domesticus

Cited by 9 publications

References 47 publications

Dietary restriction‐induced alterations on estrogen receptor alpha expression in regulating fertility in male Coturnix coturnix japonica: Relevance of Withania somnifera in modulation of inflammation and oxidative stress in testis

Dietary restriction‐induced alterations on estrogen receptor alpha expression in regulating fertility in male Coturnix coturnix japonica: Relevance of Withania somnifera in modulation of inflammation and oxidative stress in testis

Withaferin-A attenuates T2DM-induced male reproductive dysfunction mediated by ERα in brain and testes of Swiss albino mice

Ashwagandha Diminishes Hippocampal Apoptosis Induced by Microwave Radiation by Acetylcholinesterase Dependent Neuro-Inflammatory Pathway in Male Coturnix coturnix Japonica

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