Stress-induced changes in the expression of antioxidant system genes for rice (Oryza sativa L.) and bread wheat (Triticum aestivum L.)

Abstract

BackgroundPlant cell metabolism inevitably forms reactive oxygen species (ROS), which can damage cells or lead to their death. The antioxidant system (AOS) evolved to eliminate a high concentration of ROS. For plants, this system consists of the seven classes of antioxidant enzymes and antioxidant compounds. Each enzymatic class contains a various number of genes which may vary from species to species. In such a multi-copy genetic system, the integration of evolutionary characteristics and expression data makes it possible to effectively predict promising breeding targets for the design of highly-yielding cultivars. In the plant cells, ROS production can increase as a result of abiotic stresses. Accordingly, AOS responds to stress by altering the expression of the genes of its components. Expression profiles of AOS enzymes, including their changes under stress, remains incomplete. A comprehensive study of the system behavior in response to stress for different species gives the key to identify the general mechanisms of AOS regulation. In this article, we studied stress-induced changes in the expression of AOS genes in photosynthetic tissues for rice and bread wheat.MethodsA meta-analysis of genome-wide transcriptome data on stress-induced changes in expression profiles of antioxidant genes using microarray and next generation sequencing (NGS) experiments from the GEO NCBI database for rice and bread wheat was carried out. Experimental study of expression changes in short (6 h) and prolonged (24 h) cold stress responses for selected AOS genes of bread wheat cultivars Saratovskaya29 and Yanetzkis Probat was conducted using qPCR.ResultsThe large-scale meta-transcriptome and complementary experimental analysis revealed a summary of fold changes in the AOS gene expression in response to cold and water deficiency for rice and bread wheat.

Exogenous Cytokinin 4PU-30 Modulates the Response of Wheat and Einkorn Seedlings to Ultraviolet B Radiation

“…However, in response to abiotic stresses, including H 2 O 2 treatment, TmCAT1 expression increased in roots but not in leaves [73]. Up to now, ten CAT genes have been identified and molecularly characterized in wheat, and a complex transcription pattern involving the up-and downregulation of different CAT genes under various conditions and stress treatments was also observed [74,75]. In our experiments, the CAT1 gene showed quite similar expression profiles in both wheat and einkorn plants, where the transcript abundance decreased after UV-B treatment.…”

Subcellular compartmentalization of the plant antioxidant system: an integrated overview

“…The authors emphasized the role of exogenous sulfur as an inductor of salt tolerance because of enhancement of SOD, POX, CAT, increasing of AsA, tocopherol, phenolics, and decreasing carotenoids and MDA during sulfur supply. The large-scale meta-transcriptome and complementary experimental analysis of antioxidant genes regulation in response to cold and water deficiency for rice and bread wheat were carried out ( Ermakov et al, 2019 ). As a result, patterns of regulation of different gene copies of AOS in response to stress were described, and cold response turned out to be more stable and homogeneous than salinity, perhaps due to peculiarities of used methods for stress conditions.…”

Transcriptomic Data Meta-Analysis Sheds Light on High Light Response in Arabidopsis thaliana L.

“…On the other hand, transcriptomic data combined with multivariate data analysis and statistics is a powerful approach for describing regulation patterns of individual gene families. For instance, such an approach was useful for studying antioxidant gene dynamics in response to stresses (drought, cold) based on transcriptomic data and qPCR gene profiling [36]. As a result, authors identified prospective antioxidant genes involved in the stress response.…”