Stress mitigating and immunomodulatory effect of dietary pyridoxine inLabeo rohita(Hamilton) fingerlings

Abstract

A 60-day experiment was carried out to delineate stress mitigating and immunomodulatory role of dietary pyridoxine (PN) in Labeo rohita ¢ngerlings exposed to endosulfan. Two hundred and seventy ¢ngerlings were randomly distributed into six treatments in triplicates. Five iso-caloric and iso-nitrogenous puri¢ed diets were prepared with graded levels of pyridoxine. Six treatment groups were T 0 (10 mg PN1without endosulfan),T 1 (0 mg PN1endosulfan), T 2 (10 mg PN1endosulfan), T 3 (50 mg PN1 endosulfan), T 4 (100 mg PN1endosulfan) and T 5 (200 mg PN1endosulfan). The role of pyridoxine on stress mitigation and immunomodulation was assessed by biochemical and haemato-immunological parameters like aspartate aminotransaminase, alanine aminotransaminase, lactate dehydrogenase, malate dehydrogenase, superoxide dismutase and catalase were signi¢cantly (Po0.05) lower while acetylcholinesterase was signi¢cantly (Po0.05) higher in pyridoxine-fed groups. Erythrocytes count, haemoglobin content and total serum protein, albumin, globulin, nitroblue tetrazolium and lysozyme activity were signi¢cantly (Po0.05) higher while cortisol and blood glucose were decreased signi¢cantly (Po0.05) in pyridoxine-fed groups. Percentage survival after challenge with Aeromonas hydrophila was highest in T 0 group. The results obtained in present study indicate that dietary pyridoxine supplementation at 100 mg PN kg À 1 diet reduces the endosulfan-induced stress and triggers immune response in L. rohita ¢ngerlings.

Dietary choline, betaine and lecithin mitigates endosulfan-induced stress in Labeo rohita fingerlings

“…Generally, LDH activity increases in stress (Vijayaraghavan and Rao 1986). In the present study, in liver LDH and MDH, activity increased significantly (P \ 0.05) in endosulfan-exposed group, suggesting that the animals were under stress induced by endosulfan (Akhtar et al 2009). Higher activity of MDH indicates greater activity of TCA cycle due to increased energy demands during stress.…”

Subcellular component of Bacillus subtilis (AN11) induces protective immunity against Aeromonas hydrophila in Labeo rohita (Ham.)

“…In the immunized and challenged group, the overall WBC count was increased significantly, and the highest value was observed in the 72 h post-challenge with A. hydrophila. There was a study that the overall WBC count in the pre-challenge and post-challenge group was determined and found that the value at pre-challenge group decreased where there was an increased amount found after A. hydrophila infection (Shahbaz Akhtar et al, 2010).…”

“…There was a study that the overall WBC count in the pre‐challenge and post‐challenge group was determined and found that the value at pre‐challenge group decreased where there was an increased amount found after A . hydrophila infection (Shahbaz Akhtar et al, 2010).…”

An Integrated biomarker approach for explaining the potency of exogenous glucose on transportation induced stress in Labeo rohita fingerlings

“…Due to the higher potency of 0.2% glucose treatment, fish has lesser demand for additional energy during the long-term transportation stress resulting in the lowest LDH and MDH activity. Variation in antioxidant enzymes activity can be used as the indicator of stress in fish 11,37 . Oxidative stress biomarker such as SOD catalyzes the conversion of the superoxide anion into hydrogen peroxide and molecular oxygen, invariably CAT activity decomposes the hydrogen peroxide into oxygen and water 38 .…”

“…Variation in antioxidant enzymes activity can be used as the indicator of stress in fish 11 , 37 . Oxidative stress biomarker such as SOD catalyzes the conversion of the superoxide anion into hydrogen peroxide and molecular oxygen, invariably CAT activity decomposes the hydrogen peroxide into oxygen and water 38 .…”