Stress Modulation in Pinus spp. Somatic Embryogenesis as Model for Climate Change Mitigation: Stress Is Not Always a Problem

Castander-Olarieta, Ander; Pereira, Cátia; Montalbán, Itziar A.; Canhoto, Jorge; Moncaleán, Paloma

doi:10.1007/978-3-030-51358-0_7

Cited by 2 publications

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“…SE is a biotechnological tool which consists in the dedifferentiation of somatic cells and their subsequent cell re-differentiation in somatic embryos (ses), through genetic reprogramming [10]. Based on the morphogenetic response, SE is widely used to obtain large amounts of cloned material from elite material in response to an external stress stimulus [11][12][13][14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Pinus spp. Somatic Embryo Conversion under High Temperature: Effect on the Morphological and Physiological Characteristics of Plantlets

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et al. 2020

Forests

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Climatic variations in the current environmental scenario require plants with tolerance to sudden changes in temperature and a decrease in water availability. Accordingly, this tolerance will enable successful plantations and the maintenance of natural and planted forests. Consequently, in the last two decades, drought tolerance and high temperatures in conifers have been an important target for morphological, physiological, and epigenetic studies. Based on this, our research team has optimized different stages of somatic embryogenesis (SE) in Pinus spp. improving the success of the process. Through this method, we can obtain a large amount of clonal material and then analyze the somatic plants under different conditions ex vitro. The analysis of the morphological and physiological parameters in somatic embryos (ses) and plants with different tolerances to abiotic stress can provide us with valuable information about the mechanisms used by plants to survive under adverse environmental conditions. Thus, the objective of this work was to evaluate the influence of high temperatures (23, 40, 50, and 60 °C, after 12 weeks, 90, 30, 5 min, respectively) on the morphology of somatic embryos obtained from Pinus radiata D.Don (Radiata pine) and Pinus halepensis Mill. (Aleppo pine). In addition, we carried out a physiological evaluation of the somatic plants of P. radiata submitted to heat and water stress in a greenhouse. We observed that the number of somatic embryos was not affected by maturation temperatures in both species. Likewise, P. radiata plants obtained from these somatic embryos survived drought and heat stress in the greenhouse. In addition, plants originating from embryonal masses (EMs) subjected to high maturation temperature (40 and 60 °C) had a significant increase in gs and E. Therefore, it is possible to modulate the characteristics of somatic plants produced by the manipulation of environmental conditions during the process of SE.

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