Stretching Single Molecules Along Unbinding and Unfolding Pathways with the Scanning Force Microscope

Samorı̀, Bruno

doi:10.1002/1521-3765(20001201)6:23<4249::aid-chem4249>3.0.co;2-y

Cited by 17 publications

(3 citation statements)

References 49 publications

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“…In recent years, atomic force microscopy (AFM) has attracted considerable attention due to its capability of sensing interactions between its cantilever tip and a substrate as well as acquiring topographic images of sample surfaces. − There has been growing recognition that AFM is a versatile tool for the direct measurements of intermolecular forces on a molecular scale, since chemical functionalization of its probe tips and substrates by self-assembled monolayers (SAMs) allows the observation of various chemical interactions with high force and spatial resolutions. In fact, the forces for several chemical interactions have been measured by means of AFM using chemically modified probe tips, − , which is termed chemical force microscopy .…”

Section: Introductionmentioning

confidence: 99%

Single Complexation Force of 18-Crown-6 with Ammonium Ion Evaluated by Atomic Force Microscopy

Kado

Kimura

2003

J. Am. Chem. Soc.

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The cation complexation force of 18-crown-6 has been measured in ethanol by means of atomic force microscopy using probe tips and mica substrates modified chemically with 18-crown-6 and ammonium ion, respectively. The specific complexation force was suppressed by free potassium ion in the measurement solution, indicating a blocking effect based on the competitive complexation of the 18-crown-6 moiety between the free ion and the ammonium ion bound to the substrate. The single complexation force of 18-crown-6 with ammonium ion was evaluated to be about 60 pN in ethanol by autocorrelation analyses of the histograms for observed forces.

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Section: Introductionmentioning

confidence: 99%

Single Complexation Force of 18-Crown-6 with Ammonium Ion Evaluated by Atomic Force Microscopy

Kado

Kimura

2003

J. Am. Chem. Soc.

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“…In the case of secondary structures where the polypeptide chain is folded into itself with re-entrant structures, like that of titin domains, the unfolding and the refolding follow different pathways. This means that they take place in nonequilibrium conditions [78]. The typical saw-tooth pattern (Figure 6A) of the unfolding is absent in the refolding force curves.…”

Section: The Mechanical Unfolding Of a Single Protein Moleculementioning

confidence: 92%

The interplay between chemistry and mechanics in the transduction of a mechanical signal into a biochemical function

Valle¹,

Sandal²,

Samorı̀³

2007

Physics of Life Reviews

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Abstract:There are many processes in biology in which mechanical forces are generated. Force-bearing networks can transduce locally developed mechanical signals very extensively over different parts of the cell or tissues. In this article we conduct an overview of this kind of mechanical transduction, focusing in particular on the multiple layers of complexity displayed by the mechanisms that control and trigger the conversion of a mechanical signal into a biochemical function. Single molecule methodologies, through their capability to introduce the force in studies of biological processes in which mechanical stresses are developed, are unveiling subtle intertwining mechanisms between chemistry and mechanics and in particular are revealing how chemistry can control mechanics. The possibility that chemistry interplays with mechanics should be always considered in biochemical studies.

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“…He and his coworkers were very active contributors to the field of morphological analysis of DNA and nucleic acids [17]. Afterwards, in Bologna, the focus of his research gradually shifted towards single-molecule studies, employing the above-mentioned method of force versus distance techniques [18], then upgraded to folding and unfolding maps by introducing velocity-and force-clamp modes [19].…”

Section: Other Working Modesmentioning

confidence: 99%

Probing Italy: A Scanning Probe Microscopy Storyline

Dinelli

Brucale

Valle

et al. 2023

Micro

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Starting from the late 1980’s, scanning probe microscopy has progressively diffused in Italy until today. In this paper, we provide a brief account of the main historical events and a current picture of the distribution of the active groups. A survey was prepared by LimeSurvey, made of six sections asking for personal and institutional data, human resources, equipment available, fields of interest, research projects, educational/dissemination activities, and two relevant publications in the last six years. It turns out that the Italian community includes more than seventy groups and two companies. It is widely diffused, although mostly concentrated near large academic and research institutions, often in locations where prominent Italian researchers have operated. This community is active in many scientific fields and can produce research of high international quality. It shows a wide competence, as proven by the list of research works published in journals ranked within the top 20% class. The diffusion of SPM microscopes in industry is still sporadic, possibly due to extensive collaborations between the research institutions and industries themselves. The authors hope that this work might be useful to the community and beyond, and that it might stimulate the formation of a more structured network.

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Stretching Single Molecules Along Unbinding and Unfolding Pathways with the Scanning Force Microscope

Cited by 17 publications

References 49 publications

Single Complexation Force of 18-Crown-6 with Ammonium Ion Evaluated by Atomic Force Microscopy

Single Complexation Force of 18-Crown-6 with Ammonium Ion Evaluated by Atomic Force Microscopy

The interplay between chemistry and mechanics in the transduction of a mechanical signal into a biochemical function

Probing Italy: A Scanning Probe Microscopy Storyline

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