Striking differences in the biological and molecular properties of onion and garlic isolates of onion yellow dwarf virus

Celli, Marcos Giovani; Torrico, A. K.; Kiehr, M; Conci, V. C.

doi:10.1007/s00705-012-1597-z

Cited by 7 publications

(8 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The potyviruses clustered in three well-supported clades, representing OYDV, SYSV, and LYSV (Figure 3). OYDV CP nucleotide sequences included in the analyses were 80.1-99.7% identical (Table S5), which is within the species demarcation limit for potyvirus species [28]. The two OYDV isolates from Queensland clustered most closely with an isolate from Western Australian garlic (98.8% identity, GenBank JN127342) and a leek isolate from Vietnam (93.3% identity, GenBank DQ925455).…”

Section: Genetic Diversity and Phylogenetic Analysis Of Viruses Present In Australian Garlic Cropsmentioning

confidence: 79%

Detection and Distribution of Viruses Infecting Garlic Crops in Australia

Cremer

Campbell

Steele

et al. 2021

Plants

View full text Add to dashboard Cite

The distribution of viruses in eastern Australian field garlic was evaluated. Detection assays were developed that involved generic RT-PCR for viruses in the Allexivirus, Carlavirus and Potyvirus genera followed by virus-specific colorimetric dot-blot hybridization. Assays targeted the potyviruses (onion yellow dwarf virus (OYDV), shallot yellow stripe virus (SYSV), and leek yellow stripe virus (LYSV)), the carlaviruses (garlic common latent virus (GCLV) and shallot latent virus (SLV)), and the allexiviruses (garlic viruses A, B, C, X (GarVA, -B, -C, -X) and shallot virus X (ShVX)). Virus incidence in crops was consistently high, with most plants infected with at least one virus from each genus. OYDV, LYSV, SLV, and GCLV were commonly detected. Three of the four allexiviruses were in all districts surveyed but varied in incidence, whereas ShVX and SYSV were not detected. There was no association between virus species complement and bulb size, indicating size is not a good predictor of the virus status of planting material. The variation of virus incidence across different Australian growing districts and in different cultivars implies multiple introductions of viruses rather than spread within the country. The genetic diversity observed within coat protein sequences of some virus species also supports multiple separate introductions.

show abstract

Section: Genetic Diversity and Phylogenetic Analysis Of Viruses Present In Australian Garlic Cropsmentioning

confidence: 79%

Detection and Distribution of Viruses Infecting Garlic Crops in Australia

Cremer

Campbell

Steele

et al. 2021

Plants

View full text Add to dashboard Cite

show abstract

“…The outgroup included the CP coding sequences of the isolates of JYMV [ 46 ] (acc # KJ701427), TuMV [ 47 ] (acc # AB701690), NYSV [ 26 ] (acc # JQ911732), and ScaMV [ 48 ] (acc # NC_003399). For NLSYV, the outgroup included those of the isolates of CyEVA [ 27 ] (acc # NC_017977), ISMV [ 49 ] (acc # NC_029076), OYDV [ 50 ] (acc # JX433020), and SYSV [ 51 ] (acc # AM267479) for NDV. The alignments were made using CLUSTAL_X2 [ 52 ] with TRANSALIGN [ 53 ] to maintain the degapped alignment of the encoded amino acids.…”

Section: Methodsmentioning

confidence: 99%

Narcissus Plants: A Melting Pot of Potyviruses

Probowati

Kawakubo

Ohshima

2022

Viruses

View full text Add to dashboard Cite

Our paper presents detailed evolutionary analyses of narcissus viruses from wild and domesticated Narcissus plants in Japan. Narcissus late season yellows virus (NLSYV) and narcissus degeneration virus (NDV) are major viruses of Narcissus plants, causing serious disease outbreaks in Japan. In this study, we collected Narcissus plants showing mosaic or striped leaves along with asymptomatic plants in Japan for evolutionary analyses. Our findings show that (1) NLSYV is widely distributed, whereas the distribution of NDV is limited to the southwest parts of Japan; (2) the genomes of NLSYV isolates share nucleotide identities of around 82%, whereas those of NDV isolates are around 94%; (3) three novel recombination type patterns were found in NLSYV; (4) NLSYV comprises at least five distinct phylogenetic groups whereas NDV has two; and (5) infection with narcissus viruses often occur as co-infection with different viruses, different isolates of the same virus, and in the presence of quasispecies (mutant clouds) of the same virus in nature. Therefore, the wild and domesticated Narcissus plants in Japan are somewhat like a melting pot of potyviruses and other viruses.

show abstract

“…Fragaria virginiana UC-12 (indicator plant) grown in a greenhouse under controlled conditions was used as negative control for virus infection (healthy control). Allium cepa L. (onion) infected with Onion yellow dwarf virus severe (OYDV-Se) was also used as negative control (heterologous control) (Celli et al, 2013).…”

Section: Plant Materialsmentioning

confidence: 99%

Development of a non-radioactive molecular hybridization probe for detecting Strawberry mottle virus in strawberry

Asinari

Cafrune

Guzmán

et al. 2016

AgriS

View full text Add to dashboard Cite

The vegetative propagation of strawberries favors transmission of systemic pathogens, such as viruses, which are one of the main yield-limiting factors for this crop. More than 20 viruses have been described as infecting this species; one of the most frequent is the Strawberry mottle virus (SMoV), which is responsible for significant economic losses. SMoV is usually detected by reverse transcription polymerase chain reaction (RT-PCR), given that serum is not available for serological tests. In this study, a non-radioactive molecular probe was developed for SMoV detection. The cDNA was synthesized by RT-PCR using specific primers designed from the 3'UTR region of the viral genome. The cloned cDNA segment was labeled and used as a probe. Six RNA extraction protocols were evaluated, and the modified cetyltrimethylammonium bromide (CTAB) method showed the highest sensitivity level. Leaves at different phenological stages and petioles were evaluated; the highest reaction was observed in old leaves and in petioles.

show abstract

Striking differences in the biological and molecular properties of onion and garlic isolates of onion yellow dwarf virus

Cited by 7 publications

References 22 publications

Detection and Distribution of Viruses Infecting Garlic Crops in Australia

Detection and Distribution of Viruses Infecting Garlic Crops in Australia

Narcissus Plants: A Melting Pot of Potyviruses

Development of a non-radioactive molecular hybridization probe for detecting Strawberry mottle virus in strawberry

Contact Info

Product

Resources

About