Stromal vascular fraction: A regenerative reality? Part 1: Current concepts and review of the literature

Nguyen, Andrew; Guo, James; Banyard, Derek A.; Fadavi, Darya; Toranto, Jason D.; Wirth, Garrett A.; Paydar, Keyianoosh Z.; Evans, Gregory R. D.; Widgerow, Alan D.

doi:10.1016/j.bjps.2015.10.015

Cited by 177 publications

(141 citation statements)

References 54 publications

Supporting

Mentioning

132

Contrasting

Unclassified

Order By: Relevance

“…Cosmetic surgery, being at the upper-end of medical expenditure, is the largest consumer of SVF and related products, but the actual scope is much wider [3]. Thus, it is unfortunate that the benefits of this very simple technology have not reached full potential.…”

Section: Isolation Of Svfmentioning

confidence: 99%

Adipose tissue-derived stromal vascular fraction in regenerative medicine: a brief review on biology and translation

2017

View full text Add to dashboard Cite

Adipose/fat tissue provides an abundant source of stromal vascular fraction (SVF) cells for immediate administration and can also give rise to a substantial number of cultured, multipotent adipose-derived stromal cells (ADSCs). Recently, both SVF and ADSCs have gained wide-ranging translational significance in regenerative medicine. Initially used for cosmetic breast enhancement, this mode of treatment has found use in many diseases involving immune disorders, tissue degeneration, and ischaemic conditions. In this review, we try to address several important aspects of this field, outlining the biology, technology, translation, and challenges related to SVF- and ADSC-based therapies. Starting from the basics of SVF and ADSC isolation, we touch upon recently developed technologies, addressing elements of novel methods and devices under development for point-of-care isolation of SVF. Characterisation of SVF cells and ADSCs is also an evolving area and we look into unusual expression of CD34 antigen as an interesting marker for such purposes. Based on reports involving different cells of the SVF, we draw a potential mode of action, focussing on angiogenesis since it involves multiple cells, unlike immunomodulation which is governed predominantly by ADSCs. We have looked into the latest research, experimental therapies, and clinical trials which are utilising SVF/ADSCs in conditions such as multiple sclerosis, Crohn’s disease, peripheral neuropathy, osteoarthritis, diabetic foot ulcer, and so forth. However, problems have arisen with regards to the lack of proper regulatory guidelines for such therapies and, since the introduction of US Food and Drug Administration draft guidelines and the Reliable and Effective Growth for Regenerative Health Options that Improve Wellness (REGROW) Act, the debate became more public with regards to safe and efficacious use of these cells.

show abstract

Section: Isolation Of Svfmentioning

confidence: 99%

Adipose tissue-derived stromal vascular fraction in regenerative medicine: a brief review on biology and translation

2017

View full text Add to dashboard Cite

show abstract

“…SVF treatment has been shown to provide therapeutic effects similar to those of ASC treatment in osteochondral defects and myocardial infarction [19, 20], and SVF cells have been demonstrated to have similar neuroprotective effects and greater immunomodulatory properties than ASCs in experimental chronic autoimmune encephalitis studies [21]. The regenerative capacity of SVF cells has been validated in various therapeutic models, such as fat grafting, multiple sclerosis, burn injury, diabetes, radiation, Crohn’s disease, cardiac disease and bone defect models [13, 14]. When applied to these models, SVF cells have shown angiogenic, immunomodulatory, differentiation, proliferation, and pro-survival properties that are important for regeneration and repair [13].…”

Section: Introductionmentioning

confidence: 99%

Rapid Induction of Osteogenic Markers in Mesenchymal Stem Cells by Adipose-Derived Stromal Vascular Fraction Cells

Choi

Shin

Lee

et al. 2017

Cell Physiol Biochem

View full text Add to dashboard Cite

Background/Aims: Stromal vascular fraction (SVF) cells are a mixed cell population, and their regenerative capacity has been validated in various therapeutic models. The purpose of this study was to investigate the regenerative mechanisms utilized by implanted SVF cells. Using an in vitro co-culture system, we sought to determine whether SVF implantation into impaired tissue affects endogenous mesenchymal stem cell (MSC) differentiation; MSCs can differentiate into a variety of cell types, and they have a strong regenerative capacity despite their low numbers in impaired tissue. Methods: Adipose-derived SVF cells obtained from four donors were co-cultured with bone marrow-derived MSCs, and the differential expression of osteogenic markers and osteogenic differentiation inducers over time was analyzed in mono-cultured MSCs and MSCs co-cultured with SVF cells. Results: The co-cultivation of MSCs with SVF cells significantly and mutually induced the expression of osteogenic-specific markers via paracrine and/or autocrine regulation but did not induce adipocyte, chondrocyte or myoblast marker expression. More surprisingly, subsequent osteogenesis and/or comparable effects were rapidly induced within 48 h. Conclusion: To the best of our knowledge, this is the first study in which osteogenesis and/or comparable effects were rapidly induced in bone marrow-derived MSCs and adipose-derived SVF cells through co-cultivation. Our findings suggest that the positive effects of SVF implantation into impaired bone may be attributed to the rapid induction of MSC osteogenesis, and the transplantation of co-cultured and preconditioned SVF cells and/or MSCs may be more effective than the transplantation of untreated cells for the treatment of bone defects.

show abstract

“…In recent years, liposuction followed by fat grafting with SVF enrichment has gained popularity in aesthetic surgery. 6–9 Several studies have showed that SVF is comparably effective as regenerative cell therapy 8,9 in treating chronic conditions ranging from arthritis, diabetes mellitus, chronic wound, breast cancer, and radiation injuries. A major concern is how to keep SVF and ASC survival during liposuction and reduce graft volume loss after fat grafting.…”

Section: Discussionmentioning

confidence: 99%

Tumescent Liposuction without Lidocaine

Goldman

Wang

Fang

et al. 2016

Plastic and Reconstructive Surgery - Global Open

View full text Add to dashboard Cite

show abstract

Stromal vascular fraction: A regenerative reality? Part 1: Current concepts and review of the literature

Cited by 177 publications

References 54 publications

Adipose tissue-derived stromal vascular fraction in regenerative medicine: a brief review on biology and translation

Adipose tissue-derived stromal vascular fraction in regenerative medicine: a brief review on biology and translation

Rapid Induction of Osteogenic Markers in Mesenchymal Stem Cells by Adipose-Derived Stromal Vascular Fraction Cells

Tumescent Liposuction without Lidocaine

Contact Info

Product

Resources

About