Strong antimicrobial activity of Hypericum perforatum L. against oral isolates of Lactobacillus spp.

Nezhad, Sahar Khadem; Zenouz, Ali Taghavi; Aghazadeh, Mohammad; Kafil, Hossein Samadi

doi:10.14715/cmb/2017.63.11.11

Cited by 18 publications

(9 citation statements)

References 31 publications

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“…In an in vitro study investigating the effectiveness of H. hypericum -mediated aPDT on E. faecalis culture in planktonic form and biofilm, a significant microbial reduction of up to 5 log counts was detected 34 . The innovative contribution of this study is that, to the best of our knowledge, H. perforatum – widely known for its anti-depressant properties – is here studied for the first time in combination with VIS + wIRA as a light source to treat in situ oral biofilms 35–38 .…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Photoinactivation Using Visible Light Plus Water-Filtered Infrared-A (VIS + wIRA) and Hypericum Perforatum Modifies In Situ Oral Biofilms

Vollmer

Al‐Ahmad

Argyropoulou

et al. 2019

Sci Rep

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Due to increasing antibiotic resistance, the application of antimicrobial photodynamic therapy (aPDT) is gaining increasing popularity in dentistry. The aim of this study was to investigate the antimicrobial effects of aPDT using visible light (VIS) and water-filtered infrared-A (wIRA) in combination with a Hypericum perforatum extract on in situ oral biofilms. The chemical composition of H. perforatum extract was analyzed using ultra-high-performance liquid chromatography coupled with high resolution mass spectrometry (UPLC-HRMS). To obtain initial and mature oral biofilms in situ, intraoral devices with fixed bovine enamel slabs (BES) were carried by six healthy volunteers for two hours and three days, respectively. The ex situ exposure of biofilms to VIS + wIRA (200 mWcm−2) and H. perforatum (32 mg ml−1, non-rinsed or rinsed prior to aPDT after 2-min preincubation) lasted for five minutes. Biofilm treatment with 0.2% chlorhexidine gluconate solution (CHX) served as a positive control, while untreated biofilms served as a negative control. The colony-forming units (CFU) of the aPDT-treated biofilms were quantified, and the surviving microorganisms were identified using MALDI-TOF biochemical tests as well as 16 S rDNA-sequencing. We could show that the H. perforatum extract had significant photoactivation potential at a concentration of 32 mg ml−1. When aPDT was carried out in the presence of H. perforatum, all biofilms (100%) were completely eradicated (p = 0.0001). When H. perforatum was rinsed off prior to aPDT, more than 92% of the initial viable bacterial count and 13% of the mature oral biofilm were killed. Overall, the microbial composition in initial and mature biofilms was substantially altered after aPDT, inducing a shift in the synthesis of the microbial community. In conclusion, H. perforatum-mediated aPDT using VIS + wIRA interferes with oral biofilms, resulting in their elimination or the substantial alteration of microbial diversity and richness. The present results support the evaluation of H. perforatum-mediated aPDT for the adjunctive treatment of biofilm-associated oral diseases.

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Section: Discussionmentioning

confidence: 99%

Antimicrobial Photoinactivation Using Visible Light Plus Water-Filtered Infrared-A (VIS + wIRA) and Hypericum Perforatum Modifies In Situ Oral Biofilms

Vollmer

Al‐Ahmad

Argyropoulou

et al. 2019

Sci Rep

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“…The improvement in similar situations has been attributed not only to a reduction in hyperglycemia, but also to an enhancement in fibroblast proliferation, collagen bundle synthesis, and revascularization of skin injuries [201], with the likely contribution of decreased oxidative stress [199]. In this context, it should also be remembered that SJW extract exhibits not only anti-inflammatory but also antimicrobial and antiviral activities [202][203][204][205]. Interestingly, Hypericum perforatum oil has been recently incorporated into chitosan films or in bilayer material to create potential new biomaterials that promote wound healing [206,207].…”

Section: Effects Of Sjw On Diabetic Complicationsmentioning

confidence: 99%

Protective Role of St. John’s Wort and Its Components Hyperforin and Hypericin against Diabetes through Inhibition of Inflammatory Signaling: Evidence from In Vitro and In Vivo Studies

Novelli

Masiello

Beffy

et al. 2020

IJMS

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Diabetes mellitus is a very common chronic disease with progressively increasing prevalence. Besides the well-known autoimmune and inflammatory pathogenesis of type 1 diabetes, in many people, metabolic changes and inappropriate lifestyle favor a subtle chronic inflammatory state that contributes to development of insulin resistance and progressive loss of β-cell function and mass, eventually resulting in metabolic syndrome or overt type 2 diabetes. In this paper, we review the anti-inflammatory effects of the extract of Hypericum perforatum L. (St. John’s wort, SJW) and its main active ingredients firstly in representative pathological situations on inflammatory basis and then in pancreatic β cells and in obese or diabetic animal models. The simultaneous and long-lasting inhibition of signal transducer and activator of transcription (STAT)-1, nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and mitogen-activated protein kinases (MAPKs)/c-jun N-terminal kinase (JNK) signaling pathways involved in pro-inflammatory cytokine-induced β-cell dysfunction/death and insulin resistance make SJW particularly suitable for both preventive and therapeutic use in metabolic diseases. Hindrance of inflammatory cytokine signaling is likely dependent on the hyperforin content of SJW extract, but recent data reveal that hypericin can also exert relevant protective effects, mediated by activation of the cyclic adenosine monophosphate (cAMP)/protein kinase cAMP-dependent (PKA)/adenosine monophosphate activated protein kinase (AMPK) pathway, against high-fat-diet-induced metabolic abnormalities. Actually, the mechanisms of action of the two main components of SJW appear complementary, strengthening the efficacy of the plant extract. Careful quantitative analysis of SJW components and suitable dosage, with monitoring of possible drug–drug interaction in a context of remarkable tolerability, are easily achievable pre-requisites for forthcoming clinical applications.

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“…Then, the optical density (OD) of the solution was read by an ELISA Reader at 570- and 490-nm wavelengths. 12 The same procedures were repeated at 48- and 72-hour and 7-day intervals ( Figure 1 , 2 ).…”

Section: Methodsmentioning

confidence: 99%

Effect of incorporation of triphala into AH26 sealer on its cytotoxicity at different intervals

Eskandarinezhad

Haghighi

Khademnezhad

et al. 2020

J Dent Res Dent Clin Dent Prospects

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Background. One of the essential properties of sealers used during endodontic treatment is their biocompatibility. Different materials are added to these sealers to improve their properties, including antibacterial activity. In recent years, there has been an increase in interest in the use of herbal medicines. This study aimed to evaluate the effect of incorporating triphala into AH26 sealer on its cytotoxicity on gingival fibroblasts at different intervals after mixing. Methods. In the present in vitro study, the cytotoxicity of AH26 sealer was evaluated once in its pure form and once after mixing it with triphala at 24-, 48-, and 72-hour, and 7-day intervals after mixing using the standard MTT assay protocol on gingival fibroblasts. Results. Two-way ANOVA was used to evaluate the effect of groups on the mean changes in cytotoxicity at different time intervals at a significance level of P<0.05. The results showed that the incorporation of triphala into the AH26 sealer did not increase or decrease its cytotoxicity (P=0.909). Besides, there was a decrease in cytotoxicity in both study groups. However, there was a relative increase in the sealers’ cytotoxicity in both groups in the first 72 hours (P<0.0001). Conclusion. Considering the well-established antibacterial properties of triphala in our previous study, the present study’s results showed that the incorporation of triphala into the AH26 sealer did not increase the cytotoxicity of the sealer. Therefore, it can be incorporated into the AH26 sealer to improve the other properties of the sealer, including its antibacterial activity.

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Strong antimicrobial activity of Hypericum perforatum L. against oral isolates of Lactobacillus spp.

Cited by 18 publications

References 31 publications

Antimicrobial Photoinactivation Using Visible Light Plus Water-Filtered Infrared-A (VIS + wIRA) and Hypericum Perforatum Modifies In Situ Oral Biofilms

Antimicrobial Photoinactivation Using Visible Light Plus Water-Filtered Infrared-A (VIS + wIRA) and Hypericum Perforatum Modifies In Situ Oral Biofilms

Protective Role of St. John’s Wort and Its Components Hyperforin and Hypericin against Diabetes through Inhibition of Inflammatory Signaling: Evidence from In Vitro and In Vivo Studies

Effect of incorporation of triphala into AH26 sealer on its cytotoxicity at different intervals

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