Strontium inhibits osteoclastogenesis by enhancing LRP6 and β-catenin-mediated OPG targeted by miR-181d-5p

Sun, Tianhao; Li, Zhaoyang; Zhong, Xing; Cai, Zhe; Ning, Ziyu; Hou, Tianheng; Xiong, Lei; Yuan, Feng; Leung, Frankie; Lu, Wenqiang; Peng, Songlin

doi:10.1007/s12079-018-0478-y

Cited by 14 publications

(9 citation statements)

References 46 publications

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“…See Table 1 for information about miR‐181 targets in each differentiation lineage. References: [1] (H. Li et al, 2013), [2] (Ouyang et al, 2016), [3] (Z. Zhang et al, 2019), [4] (Knarr et al, 2019), [5] (Chen, Shi, et al, 2016), [6] (Chen, Ning, et al, 2016), [7] (Henao‐Mejia et al, 2013), [8] (C. Z. Chen et al, 2004), [9] (Gabler et al, 2015), [10] (P. Y. Lv, Xu et al, 2020), [11] (Barter et al, 2015), [12] (Vail et al, 2022), [13] (Melnik et al, 2021), [14] (Bakhshandeh, Soleimani, Paylakhi, et al, 2012), [15] (Q. Zhang et al, 2022), [16] (R. Su et al, 2015), [17] (X. Li, Zhang, et al, 2012), [18] (Naguibneva et al, 2006), [19] (Wei et al, 2016), [20] (P. Yuan et al, 2022), [21] (He et al, 2022), [22] (Y. Wang et al, 2020), [23] (Z. Zhao et al, 2019), [24] (Cichocki et al, 2011), [25] (Bhushan et al, 2013), [26] (N. Liu et al, 2020), [27] (Bakhshandeh, Soleimani, Hafizi, et al, 2012), [28] (Zheng, Liu, Tycksen, Nunley and McAlinden 2019), [29] (P. Qi et al, 2021), [30] (P. Y. Lv, Gao, et al, 2020), [31] (Ma et al, 2020), [32] (Y. Liu et al, 2021), [33] (Xie et al, 2018), [34] (B. Zhang et al, 2021), [35] (Shao et al, 2015), [36] (Shao et al, 2018), [37] (Fu et al, 2021), [38] (Han et al, 2020), [39] (Yu et al, 2021), [40] (T. Sun et al, 2019), [41] (G. Liu et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

“…In other studies, antiosteoclastogenic function was reported following overexpression of miR‐181b (Han et al, 2020) and miR‐181c (Yu et al, 2021). For miR‐181d, one report described mimic‐induced enhancement of osteoclast differentiation via targeting and suppression of OPG (T. Sun et al, 2019).…”

Section: Expression and Function In Skeletal Cell Differentiationmentioning

confidence: 99%

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The miR‐181 family: Wide‐ranging pathophysiological effects on cell fate and function

Bell-Hensley

Das

McAlinden

2023

Journal Cellular Physiology

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MicroRNAs (miRNAs) are epigenetic regulators that can target and inhibit translation of multiple mRNAs within a given cell type. As such, a number of different pathways and networks may be modulated as a result. In fact, miRNAs are known to regulate many cellular processes including differentiation, proliferation, inflammation, and metabolism. This review focuses on the miR‐181 family and provides information from the published literature on the role of miR‐181 homologs in regulating a range of activities in different cell types and tissues. Of note, we have not included details on miR‐181 expression and function in the context of cancer since this is a broad topic area requiring independent review. Instead, we have focused on describing the function and mechanism of miR‐181 family members on differentiation toward a number of cell lineages in various non‐neoplastic conditions (e.g., immune/hematopoietic cells, osteoblasts, osteoclasts, chondrocytes, adipocytes). We have also provided information on how modulation of miR‐181 homologs can have positive effects on disease states such as cardiac abnormalities, pulmonary arterial hypertension, thrombosis, osteoarthritis, and vascular inflammation. In this context, we have used some examples of FDA‐approved drugs that modulate miR‐181 expression. We conclude by discussing some common mechanisms by which miR‐181 homologs appear to regulate a number of different cellular processes and how targeting specific miR‐181 family members may lead to attractive therapeutic approaches to treat a number of human disease or repair conditions, including those associated with the aging process.

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Section: Discussionmentioning

confidence: 99%

Section: Expression and Function In Skeletal Cell Differentiationmentioning

confidence: 99%

The miR‐181 family: Wide‐ranging pathophysiological effects on cell fate and function

Bell-Hensley

Das

McAlinden

2023

Journal Cellular Physiology

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“…The reduced positivity of the OC differentiation markers in the case of Coll/MBG_Sr4% material may be due to the presence of Sr 2+ . Indeed, Sr 2+ interferes with OC differentiation by acting on several molecular pathways, as shown by Sun et al, who found fewer TRAP-positive cells and fewer multinuclear cells in Sr 2+ -treated cells [ 49 ]. Thus, the marked TRAP-positive response of pre-OCs on Coll/nano-HA material may be related to the absence of Sr 2+ .…”

Section: Discussionmentioning

confidence: 99%

Assessment of Collagen-Based Nanostructured Biomimetic Systems with a Co-Culture of Human Bone-Derived Cells

Borciani

Montalbano

Melo

et al. 2021

Cells

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Osteoporosis is a worldwide disease resulting in the increase of bone fragility and enhanced fracture risk in adults. In the context of osteoporotic fractures, bone tissue engineering (BTE), i.e., the use of bone substitutes combining biomaterials, cells, and other factors, is considered a potential alternative to conventional treatments. Innovative scaffolds need to be tested in in vitro systems where the simultaneous presence of osteoblasts (OBs) and osteoclasts (OCs), the two main players of bone remodeling, is required to mimic their crosstalk and molecular cooperation. To this aim, two composite materials were developed, based on type I collagen, and containing either strontium-enriched mesoporous bioactive glasses or rod-like hydroxyapatite nanoparticles. The developed nanostructured systems underwent genipin chemical crosslinking and were then tested with an indirect co-culture of human trabecular bone-derived OBs and buffy coat-derived OC precursors, for 2–3 weeks. The favorable structural and biological properties of the materials proved to successfully support the viability, adhesion, and differentiation of cells, encouraging a further investigation of the developed bioactive systems as biomaterial inks for the 3D printing of more complex scaffolds for BTE.

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“…On day 2, the medium was replaced with fresh medium consisting of M-CSF, R.A.N.K.L., and sodium butyrate. Photos were captured using inverted microscopy Zeiss microscope and imaging system (Carl Zeiss Inc., Germany) [ 42 ].…”

Section: Methodsmentioning

confidence: 99%

Determination of Therapeutic and Safety Effects of Zygophyllum coccineum Extract in Induced Inflammation in Rats

Yosri

Elaasser

Abdel‐Aziz

et al. 2022

BioMed Research International

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Background. Z. coccineum is a facultative plant with many medicinal applications. This study examined the anti-inflammatory activity of Zygophyllum coccineum (Z. coccineum) in an arthritis animal model. Materials and Methods. Seventy-Six Wistar Albino rats of either sex randomly divided into six groups (12/each). The inflammation model was done using Complete Freund’s Adjuvant in albino rats. The anti-inflammatory activities of the extract were estimated at different dose levels (15.6, 31, and 60 mg/kg) as well as upon using methotrexate (MTX) as a standard drug (0.3 mg/kg). Paw volume and arthritis index scores have been tested in all examined animals’ treatments. Histological examination of joints was also performed. Flow cytometric studies were done to isolated osteoclasts. Cytokines assay as well as biochemical testing was done in the examined samples. Results. In vitro studies reported an IC50 of 15.6 μg/ml for Z. coccineum extract in lipoxygenase inhibition assay (L.O.X.). Moreover, it could be noticed that isorhamnetin-3-O-glucoside, tribuloside, and 7-acetoxy-4-methyl coumarin were the most common compounds in Z. coccineum extract separated using L.C.–ESI-TOF–M.S. (liquid chromatography-electrospray ionization ion-trap time-of-flight mass spectrometry). Microscopic examinations of synovial tissue and hind limb muscles revealed the effect of different doses of Z. coccineum extract on restoring chondrocytes and muscles structures. Osteoclast size and apoptotic rate examinations revealed the protective effect of Z. coccineum extract on osteoclast. The results upon induction of animals and upon treatment using of MTX significantly increased apoptotic rate of osteoclast compared to control, while using of 15.6 μg/ml. for Z. coccineum extract lead to recover regular apoptotic rate demonstrating the protective effect of the extract. Z. coccineum extract regulated the secretion of proinflammatory and anti-inflammatory cytokines. Biochemical tests indicated the safety of Z. coccineum extract on kidney and liver functions. Conclusion. Z. coccineum extract has efficient and safe anti-inflammatory potential in an induced rat model.

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Strontium inhibits osteoclastogenesis by enhancing LRP6 and β-catenin-mediated OPG targeted by miR-181d-5p

Cited by 14 publications

References 46 publications

The miR‐181 family: Wide‐ranging pathophysiological effects on cell fate and function

The miR‐181 family: Wide‐ranging pathophysiological effects on cell fate and function

Assessment of Collagen-Based Nanostructured Biomimetic Systems with a Co-Culture of Human Bone-Derived Cells

Determination of Therapeutic and Safety Effects of Zygophyllum coccineum Extract in Induced Inflammation in Rats

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