Structural analysis and differentiation of reducing and nonreducing neutral model starch oligosaccharides by negative-ion electrospray ionization ion-trap mass spectrometry

Čmelík, Richard; Chmelı́k, Josef

doi:10.1016/j.ijms.2010.01.001

Cited by 20 publications

(8 citation statements)

References 33 publications

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“…Fig. 2B shows the Domon and Costello nomenclature for the fragmentation of RFOs (DP3-DP5), which are in accordance with the previous studies [23,24].…”

Section: Identification Of Rfossupporting

confidence: 89%

Preparation and purification of raffinose family oligosaccharides from Rehmannia glutinosa Libosch. by fast protein liquid chromatography coupled with refractive index detection

Zong

Cheong

et al. 2014

Separation and Purification Technology

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“…Fig. 2B shows the Domon and Costello nomenclature for the fragmentation of RFOs (DP3-DP5), which are in accordance with the previous studies [23,24].…”

Section: Identification Of Rfossupporting

confidence: 89%

Preparation and purification of raffinose family oligosaccharides from Rehmannia glutinosa Libosch. by fast protein liquid chromatography coupled with refractive index detection

Zong

Cheong

et al. 2014

Separation and Purification Technology

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“…In resume, the MS data showed that the dry heating of OS and PS samples caused not only depolymerization but also dehydration, effects which are described in the literature for similar compounds (Chiku, Nishimoto, & Kitaoka, 2010;Čmelík & Chmelík, 2010;Golon, González, Dávalos, & Kuhnert, 2013). In the case of OS samples, it was also able to induce polymerization (Moreira et al, 2011) and confirmed by Golon & Kuhnert (2012).…”

Section: Esi-ms Of Ps After Thermal Treatmentmentioning

confidence: 55%

Formation of type 4 resistant starch and maltodextrins from amylose and amylopectin upon dry heating: A model study

Nunes

Lopes

Moreira

et al. 2016

Carbohydrate Polymers

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Starch is one of the main components of human diet. During food processing, starch is submitted to high temperatures in the presence or absence of water. Thus, the main goal of this work was to identify structural modifications caused by dry heating in starch polysaccharides (amylose and amylopectin) and structurally related oligosaccharides, maltotetraose (M4) and glucosyl-maltotriose (GM3), simulating processing conditions. The structural modifications were evaluated by methylation analysis, electrospray mass spectrometry (ESI-MS), tandem mass spectrometry (ESI-MS/MS) and anionic chromatography after in vitro enzymatic digestion. Dry heating promoted dehydration, depolymerization, as well as changes in Glc glycosidic linkage positions and anomeric configuration. In oligosaccharides, polymerization was also observed. All these changes resulted in a lower in vitro digestibility, suggesting that dry heating of starch polysaccharides and related oligosaccharides may be associated with the formation of type 4 resistant starch and maltodextrins, non-digestible carbohydrates that are responsible for beneficial effects in human intestinal tract.

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“…A high resolution QTOF‐MS system (impact HD, Bruker Daltonik GmbH, Bremen, Germany) equipped with an ESI source was operated in negative ion mode . The mass range was set at m / z 100–1500 in the full‐scan mode.…”

Section: Methodsmentioning

confidence: 99%

Preparation and identification of oligosaccharides in lotus seeds and determination of their distribution in different parts of lotus

Chen

Liang³

et al. 2018

Electrophoresis

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Three fractions (I-III) were separated from crude oligosaccharides of lotus seeds by fast protein liquid chromatography with final purity of 97.6, 96.3, and 96.8%, respectively. The fractions were identified as sucrose, raffinose, and stachyose by using TLC, HPLC with charged aerosol detector (CAD), LC-MS, and methylation analysis. Subsequently sucrose and raffinose family oligosaccharides (RFOs) with degree of polymerization (DP) 3-5 (raffinose, stachyose, and verbascose) have been quantified by HPLC-CAD for the first time. All calibration curves for investigated analytes showed good linear regression (R > 0.9952). Their limit of detection and limit of quantity were in the ranges 0.14-0.28 and 0.36-0.48 μg/mL, respectively. The recoveries ranged from 96.6 to 103.4%. The contents of sucrose and RFOs DP3-DP5 were different in lotus seeds and other parts of lotus samples, but similar in their own variety. Additionally, the distribution of RFOs in different parts of lotus were also compared and the results indicated that RFOs might be mainly synthesized in lotus seeds. This work is helpful for understanding the way of biosynthesis of RFOs in lotus as well as quality control of plants containing RFOs.

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Structural analysis and differentiation of reducing and nonreducing neutral model starch oligosaccharides by negative-ion electrospray ionization ion-trap mass spectrometry

Cited by 20 publications

References 33 publications

Preparation and purification of raffinose family oligosaccharides from Rehmannia glutinosa Libosch. by fast protein liquid chromatography coupled with refractive index detection

Preparation and purification of raffinose family oligosaccharides from Rehmannia glutinosa Libosch. by fast protein liquid chromatography coupled with refractive index detection

Formation of type 4 resistant starch and maltodextrins from amylose and amylopectin upon dry heating: A model study

Preparation and identification of oligosaccharides in lotus seeds and determination of their distribution in different parts of lotus

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