Structural analysis and immunostimulatory activity of glycopeptides from Paecilomyces sinensis

Abstract

The parasitic fungus, Paecilomyces sinensis, is used to produce Cordyceps materials as a succedaneum of natural Cordyceps sinensis (C. sinensis) in China. In this work, a glycopeptide (CPS-II) was isolated and purified from Paecilomyces sinensis. The result of HPLC indicated that CPS-II was a glycopeptide. The estimated average molecular weight of CPS-II was 2 × 10(6) Da. FTIR, methylation, periodate oxidation, Smith degradation, (1)H NMR, (13)C NMR and CD were used for its structural analysis. The glycopeptide CPS-II was mainly composed of (1 → 3), (1 → 4) connected glucose and galactose as the backbone, there are (1 → 2,3,6) connected glucose, (1 → 3,6) connected mannose, and (1 → 6) connected galactose. Cell proliferation assay and morphological observations indicated that in a certain range of concentrations and time, CPS-II can significantly improve the proliferation activity of RAW264.7 cells.

Preliminary characterization and immunostimulatory activity of a novel functional polysaccharide from Astragalus residue fermented by Paecilomyces sinensis

“…Zhu et al also studied a polysaccharide from Paecilomyces sinensis (CPS). 39 Those results indicated that CPS was a kind of glycopeptide with an average molecular weight of 200 kDa and represented an a-pyran type. CPS-II mainly contained xylose, mannose, glucose, and galactose with a molar ratio of 3.7 : 12.2 : 33 : 48.…”

Immunostimulatory activity of glycopeptides from Paecilomyces sinensis under normal and cyclophosphamide induced immunosuppressive conditions in mice models

“…[3][4][5][6][7][8][9][10][11][12][13][14][15] Our previous work revealed the structural analysis of glycopeptides from Paecilomyces sinensis and demonstrated that they can activate the macrophage RAW 264.7 through enhanced proliferation and phagocytosis activity in vitro. 16 The aim of this study was to demonstrate immune responses induced by CTX in vivo. However, due to excessive exploitation and utilization, the resources of Cordyceps are greatly decreased and researchers are faced with the situation of a short supply.…”

Purification and Characterization of a New Lectin from Loach Skin Mucus

“…e absorption band at 1651.64 cm − 1 (amide I band) and 1536.44 (amide II band) cm − 1 were important characteristic absorption bands of protein, which indicated C�O stretching vibration, C-N stretching vibration, and N-H stretching vibration, respectively. e three absorption bands at 1500-1300 cm − 1 were triggered by the bending vibration of C-H, and two absorption bands at 1026-1152 cm − 1 suggested the existence of pyranose [42]. Besides, α-type glycosidic linkage in the LML was revealed by the absorption band at 879.22 cm − 1 [43].…”