Structural and Functional Changes and Possible Molecular Mechanisms in Aged Skin

Lee, Hyun Ji; Hong, Young-Joo; Kim, Miri

doi:10.3390/ijms222212489

Cited by 105 publications

(100 citation statements)

References 146 publications

(226 reference statements)

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“…The characteristic aging-associated decline of the skin barrier ( 11 ) was also reversed, as indicated by a 2.9-fold increase in the protein expression of filaggrin, a key hygroscopic protein that determines epidermal barrier function, in OiY xenotransplants ( P < 0.01) (Fig. 3A).…”

Section: Resultsmentioning

confidence: 94%

“…2D). In addition, three other key parameters that progressively decline in aging human skin, peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC1α), sirtuin-1 (SIRT1), and mitochondrially encoded cytochrome c oxidase (MTCO-1) ( 11 , 16 – 18 ), were significantly up-regulated in the epidermis of OiY compared with OiO xenotransplants and pretransplantation aged human skin (Fig. 2, E to H).…”

Section: Resultsmentioning

confidence: 98%

“…Thirty days after engrafting aged human skin onto 14 ± 3.2–month–old SCID/beige mice [“old-in-old” design (OiO)] (Fig. 1 and table S1), the phenotype and all assessed aging-associated readout parameters of aged human epidermis ( 11 ) had been fully maintained (Figs. 2 and 3 and fig.…”

Section: Resultsmentioning

confidence: 99%

“…In addition, we performed single or double IHC/IF for the following markers: Ki-67 (proliferation) ( 10 ); melan A (melanocytes) ( 13 ); filaggrin (epidermal barrier) ( 11 ); CD31 (endothelial cells) ( 56 ); c-Kit; tryptase/HLA-A, HLA-B, and HLA-C (mast cells) ( 27 ); VEGF-A ( 47 ); VEGF-A/cytokeratin-10 ( 52 ); VEGF-A/CD68 (macrophages) ( 53 ); and VEGF-A/CD42b (platelets) ( 54 ). Human skin xenotransplants were examined before and 30 days after transplantation (for details on primary and secondary antibodies, controls, and explanations, see the Supplementary Materials and Methods, tables S4 and S5).…”

Section: Methodsmentioning

confidence: 99%

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Human organ rejuvenation by VEGF-A: Lessons from the skin

et al. 2022

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Transplanting aged human skin onto young SCID/beige mice morphologically rejuvenates the xenotransplants. This is accompanied by angiogenesis, epidermal repigmentation, and substantial improvements in key aging-associated biomarkers, including ß-galactosidase, p16 ink4a , SIRT1, PGC1α, collagen 17A, and MMP1. Angiogenesis- and hypoxia-related pathways, namely, vascular endothelial growth factor A (VEGF-A) and HIF1A, are most up-regulated in rejuvenated human skin. This rejuvenation cascade, which can be prevented by VEGF-A–neutralizing antibodies, appears to be initiated by murine VEGF-A, which then up-regulates VEGF-A expression/secretion within aged human skin. While intradermally injected VEGF-loaded nanoparticles suffice to induce a molecular rejuvenation signature in aged human skin on old mice, VEGF-A treatment improves key aging parameters also in isolated, organ-cultured aged human skin, i.e., in the absence of functional skin vasculature, neural, or murine host inputs. This identifies VEGF-A as the first pharmacologically pliable master pathway for human organ rejuvenation in vivo and demonstrates the potential of our humanized mouse model for clinically relevant aging research.

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Section: Resultsmentioning

confidence: 94%

Section: Resultsmentioning

confidence: 98%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Human organ rejuvenation by VEGF-A: Lessons from the skin

et al. 2022

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“…Senstant mažėja dermos tankis, storis, kolageno kiekis. Būtent šios svarbios struktūros nykimas ir sukelia odos elastingumo, stangrumo sumažėjimąšie pokyčiai pasireiškia odos raukšlėmis ir suglebimu (Lee, Hong, Kim, 2021).…”

Section: Pav Kaulinio Audinio Pokyčiai Akiduobės Srityje 2 Pav žAndik...unclassified

Untitled

2022

VTB

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Eleidinio redkolegijaŽurnale publikuojami konferencijos "VERSLAS, TECHNOLOGIJOS, BIOMEDICINA: INOVACIJŲ ĮŽVALGOS 2022" pranešimų pagrindu parengti straipsniai. Vyriausias redaktorius ir sudarytojas:Sigutė Ežerskienė -Maisto technologijų ir mitybos katedros vedėja, lektorė PirmininkėDr. Jurga Kučinskienė -Taikomosios mokslo veiklos centro vadovė, docentė Nariai: Dr. Jurij Tekutov -Inžinerijos ir informatikos katedros docentas Dr. Lionė Urmonienė -Inžinerijos ir informatikos katedros docentė Dr. Ričardas Malkus -Transporto inžinerijos katedros docentas Dr. Viktoras Liorančas -Maisto technologijų ir mitybos katedros docentas Dr. Loreta Kelpšienė -Aplinkos ir statybos inžinerijos katedros docentė Dr. Irma Spiriajevienė -Pedagogikos katedros docentė Dr. Jurgita Paužuolienė -Verslo administravimos katedros docentė Dr. Renata Arlauskienė -Kineziterapijos ir grožio terapijos katedros docentė Dr. Evelina Daugėlienė -Burnos priežiūros katedros docentė Leidinio sudarytoja ir techninė redaktorė:Vilma Jakubauskienė -Inžinerijos ir informatikos katedros lektorė Teksto redaktorės:Reda Tamelienė -Bibliotekos vadovė, lektorė Rima Ežerskienė -Pedagogikos katedros lektorėŽurnale "Verslas, technologijos, biomedicina: inovacijų įžvalgos" publikuojami straipsniai yra recenzuojami redakcinės kolegijos narių arba jų paskirtų ekspertų.Šis darbas licencijuojamas pagal Creative Commons Attribution 4.0 International License. Atskiri straipsniai yra skelbiami "Open Access" pagal "Creative Commons" licenciją CC-BY 4.0, leidžiančią neribotai naudoti, platinti ir atgaminti bet kokioje laikmenoje, su sąlyga, kad nurodytas originalus autorius ir šaltinis. Autoriai išlaiko autorių teises į savo straipsnius, tačiau suteikia Klaipėdos valstybinei kolegijai pirmojo leidinio teisę. Už straipsnių tekstų turinį atsako straipsnių autoriai ir jų vadovai

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A guideline of antiphotoaging activity of polysaccharide assay in vitro and in vivo

Qiu,

You

2023

eFood

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People have become increasingly concerned about skin beauty and health issues, especially skin photoaging. Previous studies showed that skin photoaging was characterized by skin roughness and dryness. It was caused by UV radiation‐related dysregulation of matrix metalloproteinases (MMPs) and oxidative stress. Therefore, to investigate the antiphotoaging activity of polysaccharides, it is critical to establish the skin photoaging model, including the in vitro cellular model and the in vivo animal model. The in vitro cellular model is judged by cell viability, the MMPs expression, oxidative detection, and the quantities of inflammation cytokines. Moreover, both the damage to the epidermis and dermis of nude mice and the change of collagen content in the skin are indicators of the in vivo animal model. Thus, in this guideline, we tried to summarize the practice of antiphotoaging activity for polysaccharides.

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Structural and Functional Changes and Possible Molecular Mechanisms in Aged Skin

Cited by 105 publications

References 146 publications

Human organ rejuvenation by VEGF-A: Lessons from the skin

Human organ rejuvenation by VEGF-A: Lessons from the skin

Untitled

A guideline of antiphotoaging activity of polysaccharide assay in vitro and in vivo

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