Structural and functional characterization of a plant alpha‐actinin

Persson, Karina; Backman, Lars

doi:10.1002/2211-5463.13222

Cited by 1 publication

(1 citation statement)

References 71 publications

(123 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, we have found highly conserved spectrin homologs in the plant Rhodamnia argentea that were also experimentally characterized recently. Nevertheless, the authors had no explanation for the presence of this protein in the single plant species [ 53 ]. Within the unicellular opisthokonts, both spectrin types, alpha and beta were previously identified in the Choanoflagellata Monosiga brevicolis [ 52 ].…”

Section: Resultsmentioning

confidence: 99%

Ancient Origins of Cytoskeletal Crosstalk: Spectraplakin-like Proteins Precede the Emergence of Cortical Microtubule Stabilization Complexes as Crosslinkers

Paradžik

Podgorski

Zeljko

et al. 2022

IJMS

View full text Add to dashboard Cite

Adhesion between cells and the extracellular matrix (ECM) is one of the prerequisites for multicellularity, motility, and tissue specialization. Focal adhesions (FAs) are defined as protein complexes that mediate signals from the ECM to major components of the cytoskeleton (microtubules, actin, and intermediate filaments), and their mutual communication determines a variety of cellular processes. In this study, human cytoskeletal crosstalk proteins were identified by comparing datasets with experimentally determined cytoskeletal proteins. The spectraplakin dystonin was the only protein found in all datasets. Other proteins (FAK, RAC1, septin 9, MISP, and ezrin) were detected at the intersections of FAs, microtubules, and actin cytoskeleton. Homology searches for human crosstalk proteins as queries were performed against a predefined dataset of proteomes. This analysis highlighted the importance of FA communication with the actin and microtubule cytoskeleton, as these crosstalk proteins exhibit the highest degree of evolutionary conservation. Finally, phylogenetic analyses elucidated the early evolutionary history of spectraplakins and cortical microtubule stabilization complexes (CMSCs) as model representatives of the human cytoskeletal crosstalk. While spectraplakins probably arose at the onset of opisthokont evolution, the crosstalk between FAs and microtubules is associated with the emergence of metazoans. The multiprotein complexes contributing to cytoskeletal crosstalk in animals gradually gained in complexity from the onset of metazoan evolution.

show abstract

Section: Resultsmentioning

confidence: 99%