Structural and immunological studies on the polysaccharide from spores of a medicinal entomogenous fungus Paecilomyces cicadae

Chen, Yin; Wang, Teng; Xing, Zhang; Zhang, Fuming; Linhardt, Robert J.

doi:10.1016/j.carbpol.2020.117462

Cited by 56 publications

(37 citation statements)

References 40 publications

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“…3 C ) and 1 H– 13 C spectra according to stepwise magnetization transfers from the anomeric protons. According to the data summarized in Table 1 and literatures data ( Agrawal, 1992 , Chen et al, 2021 , Xie et al, 2020 , Yu et al, 2009 ;), it has been considered that residues A , C and E were assigned as α-D-Man p , and residues B , D and F were assigned as α-D-Glc p . Meanwhile, on the basis of the transition of chemical shifts for carbon under the condition of substitution ( Rozi et al, 2019 , Zhang et al, 2021 ), it might be concluded that residues A and F was substituted at C-6 position, and residue D was substituted at C-2 position, whereas residue B was substituted at C-2 and C-6 position.…”

Section: Resultsmentioning

confidence: 99%

Biosynthesis of exopolysaccharide and structural characterization by Lacticaseibacillus paracasei ZY-1 isolated from Tibetan kefir

Xiao

Tang

et al. 2021

Food Chemistry: Molecular Sciences

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Section: Resultsmentioning

confidence: 99%

Biosynthesis of exopolysaccharide and structural characterization by Lacticaseibacillus paracasei ZY-1 isolated from Tibetan kefir

Xiao

Tang

et al. 2021

Food Chemistry: Molecular Sciences

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“…Additionally, the signal at δ H 3.74/δ C 54.4 ppm was assigned to the oxygen methyl of α -Gal p A in the C-2 or C-3 position [ 30 ]. Residue I and J were α -configuration pyranose units and were identified as →2,6)- α -Man p -1→ and →3,4)- α -Rha p -(1→ by the anomeric signals at δ H /δ C 5.05/99.62 and δ H /δ C 4.9/99.5, respectively [ 31 , 32 ]. In the DEPT-135 spectra, the signals at δ 62.64, 68.09, and 67.82 ppm were assigned to the C-5 of A, B, and C, respectively, and the signals at δ 62.11, 70.76, 62.26, and 67.2 ppm were assigned to the C-6 of D, E, F, and I, respectively.…”

Section: Resultsmentioning

confidence: 99%

Structural Characteristics, Antioxidant, and Immunostimulatory Activities of an Acidic Polysaccharide from Raspberry Pulp

et al. 2022

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The extraction and characterization of new bioactive plant-derived polysaccharides with the potential for use as functional foods and medicine have attracted much attention. In the present study, A novel acidic polysaccharide (RPP-3a) with a weight-average molecular weight (Mw) of 88,997 Da was isolated from the raspberry pulp. RPP-3a was composed of rhamnose, arabinose, galactose, glucose, mannose, and galacturonic acid at a molar ratio of 13.1:28.6:16.8:1.4:6.2:33.9. Structural analysis suggested that the RPP-3a backbone was composed of repeating units of →4)-β-Galp-(1→3,4)-α-Rhap-(1→[4)-α-GalAp-(1→4)-α-GalAp-(1→]n with branches at the C-4 position of rhamnose. The side chain of RPP-3a, containing two branch levels, was comprised of α-Araf-(1→, →5)-α-Araf-(1→, →3,5)-α-Araf-(1→, →3)-β-Galp-(1→, →3,6)-β-Galp-(1→, →4)-β-Glcp-(1→, and →2,6)-α-Manp-1→ residues. RPP-3a exhibited moderate reducing power and strong hydroxyl and superoxide anion radical scavenging abilities. RPP-3a significantly promoted the viability of RAW264.7 macrophages by increasing the production of nitric oxide (NO), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-1β (IL-1β) at both the expression and transcriptional levels. In summary, the immunostimulatory and antioxidant activities make RPP-3a a viable candidate as a health-beneficial functional dietary supplement.

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“…It is inferred from the previous report that the apparent chemical shift signal located at 5.22 ppm indicates the presence of an α‐D‐Glc p unit in RYRO1 (Matulova et al., 2011). The signal at 5.17 ppm was assigned to an α‐D‐Man p unit (Chen et al., 2021). As shown in 1 H‐NMR, RYRO1 had anomeric proton signals at 4.87, 4.71, and 4.6 ppm, indicating the presence of β configuration, and they were designated as β‐D‐Gal, β‐D‐Man, and β‐D‐Glc, respectively (Xing et al., 2015; Zhao et al., 2020).…”

Section: Resultsmentioning

confidence: 99%

Ultrasonic extraction, structural characterization, and antioxidant activity of oligosaccharides from red yeast rice

Liu

Xie

et al. 2021

Food Science & Nutrition

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Red yeast rice is consumed as a medicinal food to lower blood lipids. Besides, it is used to color food, make wine, etc. In this study, water‐soluble oligosaccharides in red yeast rice were extracted by ultrasonic‐assisted extraction method. The parameters to extract oligosaccharides from red yeast rice were optimized by the Box‐Behnken design under the following optimal extraction conditions: extraction temperature, 60°C; extraction time, 97 min; and liquid/material ratio, 25 ml/g. The structure and the antioxidant activity of the new oligosaccharide were preliminarily investigated. Total carbohydrates extracted from red yeast rice with 80% ethanol–water solution (v/v) were first removed from pigments using D101 macroporous adsorption resin. The total sugar contents were then purified by DE52 resins and Sephadex G‐25 resins to obtain red yeast rice oligosaccharides, coded as RYRO1. Structural characterization experiments indicated that RYRO1 is an oligosaccharide with a weight average molecular weight of 874 Da and a theoretical degree of polymerization of 4.86. RYRO1 is composed of mannose, glucosamine, glucose, and galactose with a molar ratio of 0.248:0.019:1:0.026. The ABTS, DPPH, and hydroxyl free radical scavenging assays showed antioxidant nature of RYRO1.

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Structural and immunological studies on the polysaccharide from spores of a medicinal entomogenous fungus Paecilomyces cicadae

Cited by 56 publications

References 40 publications

Biosynthesis of exopolysaccharide and structural characterization by Lacticaseibacillus paracasei ZY-1 isolated from Tibetan kefir

Biosynthesis of exopolysaccharide and structural characterization by Lacticaseibacillus paracasei ZY-1 isolated from Tibetan kefir

Structural Characteristics, Antioxidant, and Immunostimulatory Activities of an Acidic Polysaccharide from Raspberry Pulp

Ultrasonic extraction, structural characterization, and antioxidant activity of oligosaccharides from red yeast rice

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