Structural and interaction parameters of thermosensitive native α-elastin biohybrid microgel

Balaceanu, Andreea; Singh, Smriti; Demco, Dan E.; Möller, Martin

doi:10.1016/j.cplett.2014.08.029

Cited by 7 publications

(14 citation statements)

References 30 publications

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“…10 In the last case the amino acid side chains in the hydrophobic domains have a larger amplitude and frequency of the segmental dynamics and hence a long transverse magnetization decay. The opposite situation is valid for the local segmental dynamics that are more restricted for the hydrophobic amino acids surrounding the crosslink points leading to biexponential decay.…”

Section: H Transverse Magnetization Relaxation (T 2 )mentioning

confidence: 99%

“…However, the signals in the aliphatic region of Gly 1 and Gly 2 as well as Val 1 and Val 2 cannot be resolved under MAS conditions. 10,17,22 Morphology and the effect of cross-link density of ELR microgels by 1 …”

Section: Proton High-resolution Magic Angle Sample Spinning (Hrmas) Nmentioning

confidence: 99%

“…This is not the case for the native a-elastin where the structural heterogeneity leads to broad peaks especially in the glycine region. 10 Proton HRMAS spectra of ELR microgels reveal resolved peaks of hydrophobic valine (Val-gCH 3 ) and proline (Pro-bCH 2 ). The glycine (Gly-aCH 2 ) peak is partially superimposed on the proline (Pro-aCH), and PEG (CH 2 ) peaks.…”

Section: Proton High-resolution Magic Angle Sample Spinning (Hrmas) Nmentioning

confidence: 99%

“…Hence, not only the morphology of microgels can be investigated by transverse relaxation but also the aggregation and volume phase temperature transition (see below). 10,25 Combined effect of volume phase temperature transition (VPTT) and aggregation process by 1 H HRMAS NMR spectral integral intensities of side-chain amino acids…”

Section: H Transverse Magnetization Relaxation (T 2 )mentioning

confidence: 99%

“…For this biohybrid microgel, information about morphological and polypeptide-water interactions in the process of reversible volume phase temperature transition (VPTT) was obtained by dynamic light scattering (DLS), 1 H high-resolution magic-angle sample spinning (HRMAS) NMR spectroscopy and relaxometry. 10 Inter-protein aggregation is widely investigated mainly because of its connection to neurodegenerative diseases, 11,12 in the industrial processes for the production of therapeutic proteins as well as for the estimation of the shelf life-time of medicaments. 13,14 Such a type of aggregation is also present in recombinantly expressed human elastin polypeptides, as well as polypentapeptides and was shown to be mainly due to interactions between the hydrophobic domains.…”

Section: Introductionmentioning

confidence: 99%

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Aggregation behaviour of biohybrid microgels from elastin-like recombinamers

et al. 2016

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aInvestigation of the aggregation behavior of biohybrid microgels, which can potentially be used as drug carriers, is an important topic, because aggregation not only causes loss of activity, but also toxicity and immunogenicity. To study this effect we synthesized microgels from elastin-like recombinamers (ELRs) using the miniemulsion technique. The existence of aggregation for such biohybrid microgels at different concentrations and temperatures was studied by different methods which include dynamic light scattering (DLS), 1 H high-resolution magic angle sample spinning (HRMAS) NMR spectroscopy, relaxometry and diffusometry. A hysteresis effect was detected in the process of aggregation by DLS as a function of temperature that strongly depends on ELR microgel concentration. The aggregation process was further quantitatively analyzed by the concentration dependence of the 1 H amino-acid residue chemical shifts and microgel diffusivity measured by NMR methods using the population balance kinetic aggregation model.

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Section: H Transverse Magnetization Relaxation (T 2 )mentioning

confidence: 99%

Section: Proton High-resolution Magic Angle Sample Spinning (Hrmas) Nmentioning

confidence: 99%

Section: Proton High-resolution Magic Angle Sample Spinning (Hrmas) Nmentioning

confidence: 99%

Section: H Transverse Magnetization Relaxation (T 2 )mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Aggregation behaviour of biohybrid microgels from elastin-like recombinamers

et al. 2016

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Stimuli‐Responsive Natural Proteins and Their Applications

Sun

et al. 2021

ChemBioChem

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Natural proteins are essential biomacromolecules that fulfill versatile functions in the living organism, such as their usage as cytoskeleton, nutriment transporter, homeostasis controller, catalyzer, or immune guarder. Due to the excellent mechanical properties and good biocompatibility/biodegradability, natural protein-based biomaterials are well equipped for prospective applications in various fields. Among these natural proteins, stimuli-responsive proteins can be reversibly and precisely manipulated on demand, rendering the protein-based biomate-rials promising candidates for numerous applications, including disease detection, drug delivery, bio-sensing, and regenerative medicine. Therefore, we present some typical natural proteins with diverse physical stimuli-responsive properties, including temperature, light, force, electrical, and magnetic sensing in this review. The structure-function mechanism of these proteins is discussed in detail. Finally, we give a summary and perspective for the development of stimuli-responsive proteins.

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