Structural and phylogenetic analysis of Pto-type disease resistance gene candidates in Hevea brasiliensis

Zhai, Wenxue; Zhao, Yan; Zhang, L.X.; Li, X.J.

doi:10.4238/2014.june.10.2

Cited by 4 publications

(3 citation statements)

References 40 publications

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“…The genetic relationship among Hevea species has been revealed through the analysis of nuclear genes, internal ribosome transcription spacer and chloroplast genes ( Cheng et al., 2009 ; Tangphatsornruang et al., 2011 ; Zhai et al., 2014 ). However, research on the reconstruction plant phylogeny of mitochondrial genome sequences is scarce.…”

Section: Discussionmentioning

confidence: 99%

Mitochondrial genome variation and intergenomic sequence transfers in Hevea species

Niu,

Gao,

Liu

2024

Front. Plant Sci.

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Among the Hevea species, rubber tree (Hevea brasiliensis) is the most important source of natural rubber. In previous studies, we sequenced the complete nuclear and chloroplast genomes of Hevea species, providing an invaluable resource for studying their phylogeny, disease resistance, and breeding. However, given that plant mitochondrial genomes are more complex and more difficult to assemble than that of the other organelles, little is known about their mitochondrial genome, which limits the comprehensive understanding of Hevea genomic evolution. In this study, we sequenced and assembled the mitochondrial genomes of four Hevea species. The four mitochondrial genomes had consistent GC contents, codon usages and AT skews. However, there were significant differences in the genome lengths and sequence repeats. Specifically, the circular mitochondrial genomes of the four Hevea species ranged from 935,732 to 1,402,206 bp, with 34–35 unique protein-coding genes, 35–38 tRNA genes, and 6–13 rRNA genes. In addition, there were 17,294–46,552 bp intergenomic transfer fragments between the chloroplast and mitochondrial genomes, consisting of eight intact genes (psaA, rrn16S, tRNA-Val, rrn5S, rrn4.5S, tRNA-Arg, tRNA-Asp, and tRNA-Asn), intergenic spacer regions and partial gene sequences. The evolutionary position of Hevea species, crucial for understanding its adaptive strategies and relation to other species, was verified by phylogenetic analysis based on the protein-coding genes in the mitochondrial genomes of 21 Malpighiales species. The findings from this study not only provide valuable insights into the structure and evolution of the Hevea mitochondrial genome but also lay the foundation for further molecular, evolutionary studies, and genomic breeding studies on rubber tree and other Hevea species, thereby potentially informing conservation and utilization strategies.

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Section: Discussionmentioning

confidence: 99%

Mitochondrial genome variation and intergenomic sequence transfers in Hevea species

Niu,

Gao,

Liu

2024

Front. Plant Sci.

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“…Gen ketahanan (R gene) dikelompokkan menjadi delapan kelas berdasarkan motif domain terkonservasi pada polipeptidanya (Zhai et al, 2014). Sejumlah 91 gen ketahanan asal tanaman telah berhasil diisolasi dan dikarakterisasi (Gurunani et al, 2012), baik dari tanaman dikotil ataupun monokotil (Meyers et al, 2005).…”

Section: Pendahuluanunclassified

“…Primer degenerate spesifik Pto telah digunakan untuk mengamplifikasi bagian gen Pto dan menghasilkan Agustus 2017 amplikon sepanjang ~540 pb untuk kakao (Kurniasih, 2012) serta ~550 pb untuk karet (Zhai et al, 2014). Amplikon dari kakao dan karet tersebut merupakan bagian dari total open reading frame (ORF, 963 pb) gen Pto (Orsi et al, 2011).…”

Section: Amplifikasi Pcr Dengan Primer Spesifik Ptounclassified