Structural and physicochemical characterization of crude biosurfactant produced by Pseudomonas aeruginosa SP4 isolated from petroleum-contaminated soil

Pornsunthorntawee, Orathai; Wongpanit, Panya; Chavadej, Sumaeth; Abe, Masahiko; Rujiravanit, Ratana

doi:10.1016/j.biortech.2007.04.020

Cited by 266 publications

(141 citation statements)

References 31 publications

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“…The Oil displacement test is an indirect measurement of the surface activity of a surfactant sample tested against oil; a larger diameter represents a higher surface activity of the testing solution (Pornsunthorntawee et al, 2008). The oil spreading method is rapid and easy to carry out, requires no specialized equipment and just a small volume of sample (Płaza et al, 2006).…”

Section: Oil Displacement Testmentioning

confidence: 99%

“…Emulsification of rhamnolipids depends on the carbon sources used to produce rhamnolipids (Pornsunthorntawee et al, 2008). Six carbon sources, i.e., glucose, glycerol, sucrose, hexane, oleic acid, and olive oil were examined for their effectiveness of biosurfactant production.…”

Section: Estimation Of Emulsification Activitymentioning

confidence: 99%

“…The absorption peak around 1051cm −1 was also reported as C-O-C stretching in rhamnose (Pornsunthorntawee et al, 2008). The α-pyranyl II sorption band at 834 cm…”

Section: Fourier Transform Infrared Spectroscopy (Ftir)mentioning

confidence: 99%

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Production and characterization of di-rhamnolipid produced by Pseudomonas aeruginosa TMN

Moussa

Mohamed

Samak

2014

Braz. J. Chem. Eng.

109

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-Pseudomonas aeruginosa TMN was used to produce rhamnolipid (RL) from a variety of carbon and nitrogen substrates. The most favorable carbon sources for RL production were glucose and glycerol (both at 40 g/L), giving a RL yield of 0.3 and 0.25 g/L, respectively. Meanwhile, sodium nitrate appeared to be the preferable nitrogen source, resulting in a RL production of 0.34g/L. Rhamnolipid production from P. aeruginosa TMN was affected by temperature, pH and agitation rate, with 37 °C, pH 7 and 200 rpm agitation favorable for rhamnolipid production. Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance (NMR) and electro spray ionization-mass spectrometry (ESI-MS) analyses indicated that the purified product contained one type of commonly found rhamnolipid, which is L-rhamnosyl-L-rhamnosyl-β-hydroxydecanoyl-β-hydroxydecanoate. The rhamnolipid product can reduce the surface tension of water to 34 mN/m with a critical micelle concentration of nearly 18.75 mg/L and emulsified kerosene by 46%. P. aeruginosa TMN strain is a potential source of rhamnolipid biosurfactant, which could be used for the development of bioremediation processes in the marine environment.

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Section: Oil Displacement Testmentioning

confidence: 99%

Section: Estimation Of Emulsification Activitymentioning

confidence: 99%

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Production and characterization of di-rhamnolipid produced by Pseudomonas aeruginosa TMN

Moussa

Mohamed

Samak

2014

Braz. J. Chem. Eng.

109

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“…The maximum diameter of the clear zone was recorded under daylight. The higher the surface activity of the test solution, the larger the diameter of the clear zone [23].…”

Section: Oil Displacement Testmentioning

confidence: 99%

Biodegradation of n-hexadecane by bacterial strains B1 and B2 isolated from petroleum-contaminated soil

et al. 2012

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Two hydrocarbon-biodegrading bacterial strains, B1 and B2, were isolated from petroleum-contaminated soil collected from Tianjin, China. The strains were identified as Pseudomonas aeruginosa (B1) and Acinetobacter junii (B2). The degradation rate of n-hexadecane by B1 and B2 reached 96% and 78% respectively after 7 days, though the strains employed different mechanisms of degradation. The results showed that B2 was not able to use glucose as carbon source. B1 could produce glycolipid surfactants using glucose as the carbon source, according to the results of blue agar plate analysis and thin layer chromatography (TLC), and the bacterial culture of B1 had a high oil discharge and emulsification activity. Both B1 and B2 could produce biosurfactants with hexadecane as the sole carbon source, but their modes of action were different. The carbon source was found to affect the cell surface hydrophobicity. Cell surface hydrophobicity was poor with glucose as the carbon source, but enhanced when hexadecane was used as the carbon source.

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“…Los ramnolípidos estudiados en este trabajo son estables en un amplio rango de pH (4 a 11) en comparación con los resultados obtenidos por Pornsunthorntawee et al (2008), quienes reportan la estabilidad de los ramnolípidos, producidos por P. aeruginosa SP4 para valores de pH entre 6 y 11. Igualmente, los ramnolípidos caracterizados por Techaoei et al (2011) fueron estables sólo en el rango de pH de 6 a 10.…”

Section: Discussionunclassified

EXTRACCIÓN DE PETRÓLEO EN SUELO CONTAMINADO EMPLEANDO RAMNOLÍPIDOS PRODUCIDOS POR Pseudomonas aeruginosa ORA9

Vilasó-Cadre

Gámez

Rodríguez

2017

Rev. Int. Contam. Ambie.

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Palabras clave: remediación, hidrocarburos, diseño Box-Behnken RESUMEN La remoción de hidrocarburos empleando ramnolípidos ha mostrado potencialidades para su aplicación en tecnologías de remediación de ecosistemas impactados, así como en el aumento de la eficiencia de extracción de petróleo en pozos. En esta investigación se evaluó la remoción de hidrocarburos totales de petróleo de un suelo contaminado empleando los ramnolípidos producidos por Pseudomonas aeruginosa ORA9. Se realizó un estudio de estabilidad de los ramnolípidos considerando los factores temperatura, pH y fuerza iónica. Los ramnolípidos fueron estables en las condiciones ensayadas. Se utilizó un diseño Box-Behnken para evaluar la influencia de la temperatura, pH y tiempo de contacto, en la remoción de petróleo de suelo contaminado. Los tres factores influyeron significativamente, incrementándose la extracción al aumentar la temperatura y el tiempo de contacto y disminuir el pH. La máxima extracción alcanzada fue de un 75 %.Key words: remediation, hydrocarbons, Box-Behnken design ABSTRACT Hydrocarbon removal using rhamnolipids has shown potential for use in remediation technologies of impacted ecosystems as well as increasing the efficiency of oil extraction wells. In this research, the removal of total petroleum hydrocarbons in contaminated soil was evaluated using rhamnolipids produced by Pseudomonas aeruginosa ORA9. A stability study of rhamnolipids considering temperature, pH and ionic strength factors was performed. The rhamnolipids were stable in the tested conditions. A Box-Behnken design was employed to evaluate the influence of temperature, pH and contact time on the removal of petroleum from contaminated soil. All factors have a significative influence on the extraction of total petroleum hydrocarbons, increasing with temperature and contact time, and decreasing with pH. The maximum extraction was 75 %.

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Structural and physicochemical characterization of crude biosurfactant produced by Pseudomonas aeruginosa SP4 isolated from petroleum-contaminated soil

Cited by 266 publications

References 31 publications

Production and characterization of di-rhamnolipid produced by Pseudomonas aeruginosa TMN

Production and characterization of di-rhamnolipid produced by Pseudomonas aeruginosa TMN

Biodegradation of n-hexadecane by bacterial strains B1 and B2 isolated from petroleum-contaminated soil

EXTRACCIÓN DE PETRÓLEO EN SUELO CONTAMINADO EMPLEANDO RAMNOLÍPIDOS PRODUCIDOS POR Pseudomonas aeruginosa ORA9

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