Structural Aspects of Multistep Phosphorelay-Mediated Signaling in Plants

Pekárová, Blanka; Szmitkowska, Agnieszka; Dopitová, Radka; Degtjarik, Oksana; Žı́dek, Lukáš; Hejátko, Jan

doi:10.1016/j.molp.2015.11.008

Cited by 30 publications

(33 citation statements)

References 113 publications

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“…Our results are in agreement with those obtained with CKI1 showing interaction specificity with only three AHP among six in Arabidopsis [46]. Overall, these findings strengthen the hypothesis that HPt proteins participate to a certain level of interaction specificity within the MSP system [47,48], validating HPt2 and HPt9 as partners probably involved in poplar MSP linked to drought.…”

Section: Discussionsupporting

confidence: 92%

Functional Divergence of Poplar Histidine-Aspartate Kinase HK1 Paralogs in Response to Osmotic Stress

Héricourt

Chefdor

Djeghdir

et al. 2016

IJMS

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Previous works have shown the existence of protein partnerships belonging to a MultiStep Phosphorelay (MSP) in Populus putatively involved in osmosensing. This study is focused on the identification of a histidine-aspartate kinase, HK1b, paralog of HK1a. The characterization of HK1b showed its ability to homo- and hetero-dimerize and to interact with a few Histidine-containing Phosphotransfer (HPt) proteins, suggesting a preferential partnership in poplar MSP linked to drought perception. Furthermore, determinants for interaction specificity between HK1a/1b and HPts were studied by mutagenesis analysis, identifying amino acids involved in this specificity. The HK1b expression analysis in different poplar organs revealed its co-expression with three HPts, reinforcing the hypothesis of partnership participation in the MSP in planta. Moreover, HK1b was shown to act as an osmosensor with kinase activity in a functional complementation assay of an osmosensor deficient yeast strain. These results revealed that HK1b showed a different behaviour for canonical phosphorylation of histidine and aspartate residues. These phosphorylation modularities of canonical amino acids could explain the improved osmosensor performances observed in yeast. As conserved duplicates reflect the selective pressures imposed by the environmental requirements on the species, our results emphasize the importance of HK1 gene duplication in poplar adaptation to drought stress.

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Section: Discussionsupporting

confidence: 92%

Functional Divergence of Poplar Histidine-Aspartate Kinase HK1 Paralogs in Response to Osmotic Stress

Héricourt

Chefdor

Djeghdir

et al. 2016

IJMS

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“…Conserved amino acids and consensus motifs (N, G1, F, G2) are indicated. According to conventional terminology, the catalytic module consists of dimerization and histidine phosphotransfer domain (DHpD), and catalytic and ATP-binding domain (CAD) (Mayerhofer et al ., 2015; Pekárová et al ., 2016). Scales at the bottom of the structures indicate the length in aa number.…”

Section: Resultsmentioning

confidence: 99%

Cytokinin perception in potato: New features of canonic players

Lomin

Myakushina

Kolachevskaya

et al. 2018

Preprint

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15Potato is the most economically important non-cereal food crop. Tuber formation in potato is regulated 16 by phytohormones, cytokinins in particular. The present work was aimed to study cytokinin signal 17 perception in potato. The sequenced potato genome of doubled monoploid Phureja was used for 18 bioinformatic analysis and as a tool for identification of cytokinin receptors from autotetraploid potato 19 cv. Désirée. All basic elements of multistep phosphorelay (MSP) required for cytokinin signal 20 transduction were identified in Phureja genome, including three genes orthologous to three cytokinin 21 receptor genes (AHK 2-4) of Arabidopsis. As distinct from Phureja, autotetraploid potato contains at 22 least two allelic isoforms of each receptor type. Putative receptor genes from Désirée plants were 23 cloned, sequenced and expressed, and main characteristics of encoded proteins, firstly their consensus 24 motifs, structure models, ligand-binding properties, and the ability to transmit cytokinin signal, were 25 determined. In all studied aspects the predicted sensor histidine kinases met the requirements for 26 genuine cytokinin receptors. Expression of potato cytokinin receptors was found to be organ-specific 27 and sensitive to growth conditions, particularly to sucrose content. Our results provide a solid basis for 28 further in-depth study of cytokinin signaling system and biotechnological improvement of potato. 29 2

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“…The notion of HPts as hub proteins is a concept that emerged a few years ago with the growing amount of MSP interaction network identification [21,54,55]. As integrative proteins in MSP are able to interact with upstream and downstream partners, it was proposed that HPt proteins act as hubs within the CK signaling pathway in Arabidopsis [21].…”

Section: Discussionmentioning

confidence: 99%

“…As integrative proteins in MSP are able to interact with upstream and downstream partners, it was proposed that HPt proteins act as hubs within the CK signaling pathway in Arabidopsis [21]. This idea was slightly modulated by Pekárová et al [54] who argued that HPts are not unspecific signaling hubs but can achieve a certain level of interaction specificity. In Arabidopsis, numerous examples have unveiled the crosstalk between CK signaling and other pathways.…”

Section: Discussionmentioning

confidence: 99%

New Insight into HPts as Hubs in Poplar Cytokinin and Osmosensing Multistep Phosphorelays: Cytokinin Pathway Uses Specific HPts

Héricourt

Larcher

Chefdor

et al. 2019

Plants

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We have previously identified proteins in poplar which belong to an osmosensing (OS) signaling pathway, called a multistep phosphorelay (MSP). The MSP comprises histidine-aspartate kinases (HK), which act as membrane receptors; histidine phosphotransfer (HPt) proteins, which act as phosphorelay proteins; and response regulators (RR), some of which act as transcription factors. In this study, we identified the HK proteins homologous to the Arabidopsis cytokinin (CK) receptors, which are first partners in the poplar cytokinin MSP, and focused on specificity of these two MSPs (CK and OS), which seem to share the same pool of HPt proteins. Firstly, we isolated five CK HKs from poplar which are homologous to Arabidopsis AHK2, AHK3, and AHK4, namely, HK2, HK3a, HK3b, HK4a, HK4b. These HKs were shown to be functional kinases, as observed in a functional complementation of a yeast HK deleted strain. Moreover, one of these HKs, HK4a, was shown to have kinase activity dependent on the presence of CK. Exhaustive interaction tests between these five CK HKs and the 10 HPts characterized in poplar were performed using two-hybrid and BiFC experiments. The resulting partnership was compared to that previously identified between putative osmosensors HK1a/1b and HPt proteins. Finally, in planta coexpression analysis of genes encoding these potential partners revealed that almost all HPts are coexpressed with CK HKs in four different poplar organs. Overall, these results allowed us to unravel the common and specific partnerships existing between OS and CK MSP in Populus.

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Structural Aspects of Multistep Phosphorelay-Mediated Signaling in Plants

Cited by 30 publications

References 113 publications

Functional Divergence of Poplar Histidine-Aspartate Kinase HK1 Paralogs in Response to Osmotic Stress

Functional Divergence of Poplar Histidine-Aspartate Kinase HK1 Paralogs in Response to Osmotic Stress

Cytokinin perception in potato: New features of canonic players

New Insight into HPts as Hubs in Poplar Cytokinin and Osmosensing Multistep Phosphorelays: Cytokinin Pathway Uses Specific HPts

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