Structural basis for energy harvesting and dissipation in a diatom PSII–FCPII supercomplex

Nagao, Ryo; Kato, Kuniki; Suzuki, Takehiro; Ifuku, Kentaro; Uchiyama, Ikuo; Kashino, Yasuhiro; Dohmae, Naoshi; Akimoto, Seiji; Shen, Jian‐Ren; Miyazaki, Naoyuki; Akita, Fusamichi

doi:10.1038/s41477-019-0477-x

Cited by 115 publications

(168 citation statements)

References 38 publications

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“…4). Notably, these changes occur despite the significant differences in the structure and organisation of the diatom FCP antenna system compared to the equivalent light harvesting system in plants (Nagao et al , 2019; Pi et al , 2019). The overall rate of photosynthesis was also reduced by ppGpp accumulation (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…At the level of membrane architecture, the diatom chloroplast is surrounded by four membranes, and the thylakoids are loosely stacked with three interconnected membranes (Flori et al , 2017). Moreover, the light-harvesting complex (LHC) of diatoms is known as the Fucoxanthin Chlorophyll Protein complex (FCP), and is composed of tetramers containing chlorophyll c and the carotenoid fucoxanthin (Lepetit et al , 2012; Roding et al , 2018; Nagao et al , 2019; Pi et al , 2019). Diatoms have other specific features such as a functional urea cycle (Allen et al , 2011), a eukaryotic Entner-Doudoroff glycolytic pathway (Fabris et al , 2012) and they lack the plastid oxidative pentose phosphate pathway found in plants (Gruber & Kroth, 2017).…”

Section: Introductionmentioning

confidence: 99%

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ppGpp influences protein protection, growth and photosynthesis inPhaeodactylum tricornutum

Avilán

Lebrun

Puppo

et al. 2020

Preprint

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Chloroplasts retain elements of a bacterial stress response pathway that is mediated by the signalling nucleotides guanosine penta-and tetraphosphate, or (p)ppGpp. In the model flowering plant Arabidopsis, ppGpp acts as a potent regulator of plastid gene expression and influences photosynthesis, plant growth and development.However, little is known about ppGpp metabolism or its evolution in other photosynthetic eukaryotes.• Here, we studied the function of ppGpp in the diatom P. tricornutum using transgenic lines containing an inducible system for ppGpp accumulation. We used these lines to investigate the effects of ppGpp on growth, photosynthesis, lipid metabolism and protein expression.• We demonstrate that ppGpp accumulation reduces photosynthetic capacity and promotes a quiescent-like state with reduced proliferation and ageing. Strikingly, using non-targeted proteomics, we discovered that ppGpp accumulation also leads to the coordinated upregulation of a protein protection response in multiple cellular compartments.• Our findings highlight the importance of ppGpp as a fundamental regulator of chloroplast function across different domains of life, and lead to new questions about the molecular mechanisms and roles of (p)ppGpp signalling in photosynthetic eukaryotes.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

ppGpp influences protein protection, growth and photosynthesis inPhaeodactylum tricornutum

Avilán

Lebrun

Puppo

et al. 2020

Preprint

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“…In the centric diatom Chaetoceros gracilis , FCPII exists mainly in three types designated FCP‐A, FCP‐B, and FCP‐C . The structure of a PSII–FCPII supercomplex with FCP‐A as the dominant antenna was analyzed up to 3.0 Å resolution by cryo‐EM . Surprisingly, the structure shows that the major FCP exists in a tetramer (Fig.…”

Section: Overall Structure Of the Psii–fcpii Supercomplexmentioning

confidence: 99%

“…The PSII–FCPII dimer supercomplex contains 230 Chls a , 58 Chls c , 4 pheophytins, 124 Fxs, 20 Bcrs, and 2 Ddxs, as well as some other cofactors involved in the electron transfer chain . In addition, 20 digalactosyl diacylglycerol (DGDG), 42 monogalactosyl diacylglycerols (MGDG), 16 sulfoquinovosyl diacylglycerol (SQDG), and 30 phosphatidylglycerol (PG) molecules are found in a PSII–FCPII dimer; these lipids are mostly distributed in the interfaces between subunits (Fig.…”

Section: Overall Structure Of the Psii–fcpii Supercomplexmentioning

confidence: 99%

“…In particular, single‐particle cryo‐EM analysis has provided a powerful tool to unravel the organization of several PSII–LHC and PSI–LHC supercomplexes from different lineages of organisms from cyanobacteria to green algae and higher plants . The structure of PSII–FCPII from diatoms, however, was solved only recently , and a high‐resolution crystal structure of an isolated FCP dimer was also reported recently . These studies revealed the oligomeric states and organization of FCPII subunits around the PSII core, the distribution and detailed binding environments of Chls c and Fxs, and a huge pigment–protein network within the PSII–FCPII supercomplex, which enable us to examine the excitation energy transfer and quenching pathways in the diatom PSII–FCPII in a greater detail.…”

Section: Introductionmentioning

confidence: 99%

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Structural features of the diatom photosystem II–light‐harvesting antenna complex

Wang

Zhao

et al. 2020

The FEBS Journal

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In photosynthesis, light energy is captured by pigments bound to light-harvesting antenna proteins (LHC) that then transfer the energy to the photosystem (PS) cores to initiate photochemical reactions. The LHC proteins surround the PS cores to form PS-LHC supercomplexes. In order to adapt to a wide range of light environments, photosynthetic organisms have developed a large variety of pigments and antenna proteins to utilize the light energy efficiently under different environments. Diatoms are a group of important eukaryotic algae and possess fucoxanthin (Fx) chlorophyll a/c proteins (FCP) as antenna which have exceptional capabilities of harvesting blue-green light under water and dissipate excess energy under strong light conditions. We have solved the structure of a PSII-FCPII supercomplex from a centric diatom Chaetoceros gracilis by cryo-electron microscopy, and also the structure of an isolated FCP dimer from a pennate diatom Phaeodactylum tricornutum by X-ray crystallography at a high resolution.These results revealed the oligomerization states of FCPs distinctly different from those of LHCII found in the green lineage organisms, the detailed binding patterns of Chl c and Fxs, a huge pigment network, and extensive protein-protein, pigment-protein, and pigment-pigment interactions within the PSII-FCPII supercomplex. These results therefore provide a solid structural basis for examining the detailed mechanisms of the highly efficient energy transfer and quenching processes in diatoms.

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Genetic Regulation of Diatom Photosynthesis

Volpe,

Nymark,

Brembu

2024

Diatom Photosynthesis

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Structural basis for energy harvesting and dissipation in a diatom PSII–FCPII supercomplex

Cited by 115 publications

References 38 publications

ppGpp influences protein protection, growth and photosynthesis inPhaeodactylum tricornutum

ppGpp influences protein protection, growth and photosynthesis inPhaeodactylum tricornutum

Structural features of the diatom photosystem II–light‐harvesting antenna complex

Genetic Regulation of Diatom Photosynthesis

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