Structural Basis for the Carbohydrate Recognition of the Sclerotium rolfsii Lectin

Leonidas, D.D.; Swamy, Bale M.; Hatzopoulos, George N.; Gonchigar, Sathisha J.; Chachadi, Vishwanath B.; Zographos, S.E.; Oikonomakos, Nikos G.

doi:10.1016/j.jmb.2007.02.092

Cited by 41 publications

(40 citation statements)

References 73 publications

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“…These processes include cellular signaling, differentiation, and host-pathogen interactions through the specific recognition of glycol conjugates of the host cell membrane by lectins present on the surface of the fungal wall (Guillot and Konska 1997;Hamshou et al 2007;Khan and Khan 2011). In accordance with our study, fungal lectins have been previously detected in the mycelium and sclerotia of R. solani strains of different anastomosis groups (Kellens and Peumans 1991;Mghalu et al 2004) with a reported role in fungal growth and development (Leonidas et al 2007). Additionally, R. solani lectins were revealed to act as storage proteins (Skamnaki et al 2013).…”

Section: Carbohydrate-binding Genessupporting

confidence: 87%

Suppression subtractive hybridization and comparative expression of a pore-forming toxin and glycosyl hydrolase genes in Rhizoctonia solani during potato sprout infection

et al. 2014

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Rhizoctonia solani is a plant pathogenic fungus that causes black scurf on tubers and stem and stolon canker on underground parts of potato plant. Early in the season, the fungus attacks germinating sprouts underground before they emerge from the soil. Damage at this stage results in delayed emergence of weakened plants with poor and uneven stands. The mechanism underlying this phenomenon has been investigated in this study by coupling a cDNA-suppression subtractive hybridization (SSH) library to differential screening to identify transcripts of R. solani that are down-regulated during infection of potato sprouts. We report on the identification of 33 unique genes with functions related to carbohydrate binding, vitamin synthesis, pathogenicity, translation, ATP and nucleic acid binding and other categories. RACE-PCR was used to clone and characterize the first full-length cDNA clones, RSENDO1 and RSGLYC1 that encode for an eukaryotic delta-endotoxin CytB protein and an intracellular glycosyl hydrolase, respectively. Quantitative real-time PCR revealed the down-regulation of RSENDO1 during infection of potato sprouts and the up-regulation of RSGLYC1 when the fungus was grown on a cellulose-based nutrient medium. In contrast, additional experiments have highlighted the down-regulation of RSENDO1 when R. solani was co-cultured with the mycoparasite Stachybotrys elegans and the bacterial antagonist Bacillus subtilis B26. These results advance our understanding of R. solani-potato interaction in subterranean parts of the plant. Such approaches could be considered in building an efficient integrated potato disease management program.

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Section: Carbohydrate-binding Genessupporting

confidence: 87%

Suppression subtractive hybridization and comparative expression of a pore-forming toxin and glycosyl hydrolase genes in Rhizoctonia solani during potato sprout infection

et al. 2014

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“…The lectin isolated from Rhizoctonia crocorum and the lectin from Agaricus blazei are both tetramers consisting of 11 kDa and 16 kDa subunits, respectively [22,23]. Similarly, a lectin isolated from Sclerotium rolfsii is a homo dimer with subunit mass of 17 kDa, which shows pH dependent oligomerization [24,25]. The N-terminal amino acid in RBL is blocked which was indicated by its failure to release dansyl derivative.…”

Section: Discussionmentioning

confidence: 99%

A potent mitogenic lectin from the mycelia of a phytopathogenic fungus, Rhizoctonia bataticola, with complex sugar specificity and cytotoxic effect on human ovarian cancer cells

et al. 2010

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A lectin with strong mitogenic activity towards human peripheral blood mononuclear cells (PBMCs) and cytotoxic effect on human ovarian cancer cells has been purified from the mycelium of a phytopathogenic fungus, Rhizoctonia bataticola, using ion exchange chromatography and affinity chromatography on asialofetuin-Sepharose. The lectin, termed RBL, is a tetramer of 11-kDa subunits and has unique amino acid sequence at its blocked N-terminus. The purified RBL was blood group nonspecific and its hemagglutination activity was inhibited by mucin (porcine stomach), fetuin (fetal calf serum) and asialofetuin. Glycan array analysis revealed high affinity binding of RBL towards N-glycans and also the glycoproteins containing complex N-glycan chains. Interestingly, the lectin showed high affinity for glycans which are part of ovarian cancer marker CA125, a high molecular weight mucin containing high mannose and complex bisecting type N-linked glycans as well core 1 and 2 type O-glycans. RBL bound to human PBMCs eliciting strong mitogenic response, which could be blocked by mucin, fetuin and asialofetuin demonstrating the carbohydrate-mediated interaction with the cells. Analysis of the kinetics of binding of RBL to PBMCs revealed a delayed mitogenic response indicating a different signaling pathway compared to phytohemagglutinin-L. RBL had a significant cytotoxic effect on human ovarian cancer cell line, PA-1.

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“…1). SRL was shown to be a dimer but can, from a structural point of view, form similar tetramers as other representatives (Birck et al 2004;Bovi et al 2011;Carrizo et al 2005;Leonidas et al 2007).…”

Section: Actinoporin-type Lectinsmentioning

confidence: 99%

“…It was suggested that the archetypal actinoporin fold is used for specific binding to various molecules at the plasma membrane surface (Kristan et al 2009). Characterized representatives of the actinoporin-type mushroom lectin family include XCL, ABL (ABA), SRL, and BEL from the basidiomycetes Xerocomus (Boletus) chrysenteron, Agaricus bisporus, Sclerotium (Athelia) rolfsii, and B. edulis (Birck et al 2004;Bovi et al 2011;Carrizo et al 2005;Leonidas et al 2007) and AOL 2 and TAP1 from the ascomycetes Arthrobotrys oligospora and Sordaria macrospora (Nowrousian and Cebula 2005;Rosen et al 1996b). These representatives of basidiomycetes share 53 to 82 % sequence identity (67 to 89 % similarity), while AOL 2 is approximately 45 % identical (62 % similar) to them and TAP1 only 34 % identical (50 % similar).…”

Section: Actinoporin-type Lectinsmentioning

confidence: 99%

“…ABL is a dual specificity lectin harboring separate binding sites for Gal-β1,3-GalNAc (T-or Tn-antigen) and N-acetylglucosamine (GlcNAc) (Nakamura-Tsuruta et al 2006). Similarly, structural studies on SRL revealed two carbohydrate-binding sites: a primary one for GalNAc and secondary one for GlcNAc (Leonidas et al 2007). Based on sequence homology and functional studies, the GlcNAcbinding site is conserved in XCL, AOL 2 , and BEL but not in TAP1 .…”

Section: Actinoporin-type Lectinsmentioning

confidence: 99%

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Entomotoxic and nematotoxic lectins and protease inhibitors from fungal fruiting bodies

Sabotič

Ohm

Künzler

2015

Appl Microbiol Biotechnol

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Fruiting bodies or sporocarps of dikaryotic (ascomycetous and basidiomycetous) fungi, commonly referred to as mushrooms, are often rich in entomotoxic and nematotoxic proteins that include lectins and protease inhibitors. These protein toxins are thought to act as effectors of an innate defense system of mushrooms against animal predators including fungivorous insects and nematodes. In this review, we summarize current knowledge about the structures, target molecules, and regulation of the biosynthesis of the best characterized representatives of these fungal defense proteins, including galectins, beta-trefoil-type lectins, actinoporin-type lectins, beta-propeller-type lectins and beta-trefoil-type chimerolectins, as well as mycospin and mycocypin families of protease inhibitors. We also present an overview of the phylogenetic distribution of these proteins among a selection of fungal genomes and draw some conclusions about their evolution and physiological function. Finally, we present an outlook for future research directions in this field and their potential applications in medicine and crop protection.

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Structural Basis for the Carbohydrate Recognition of the Sclerotium rolfsii Lectin

Cited by 41 publications

References 73 publications

Suppression subtractive hybridization and comparative expression of a pore-forming toxin and glycosyl hydrolase genes in Rhizoctonia solani during potato sprout infection

Suppression subtractive hybridization and comparative expression of a pore-forming toxin and glycosyl hydrolase genes in Rhizoctonia solani during potato sprout infection

A potent mitogenic lectin from the mycelia of a phytopathogenic fungus, Rhizoctonia bataticola, with complex sugar specificity and cytotoxic effect on human ovarian cancer cells

Entomotoxic and nematotoxic lectins and protease inhibitors from fungal fruiting bodies

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