Structural changes of bacterial nanocellulose pellicles induced by genetic modification of Komagataeibacter hansenii ATCC 23769

Jacek, Paulina; Ryngajłło, Małgorzata; Bielecki, Stanisław

doi:10.1007/s00253-019-09846-4

Cited by 31 publications

(18 citation statements)

References 83 publications

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“…Jacek et al investigated the effect of motility genes (motA and motB) on the structure of cellulose by disruption or overexpression of these genes in K. hansenii. Microscopic analysis of the BC membrane produced in overexpressed motility genes exhibited a substantial loosening of intra-membrane structure and fiber thickening was observed [46]. In contrast, disruption of these genes resulted in denser and more compact BC as a result of reduction in motility, and improvement in their mechanical properties [47].…”

Section: Product Levelmentioning

confidence: 99%

Systematic Understanding of Recent Developments in Bacterial Cellulose Biosynthesis at Genetic, Bioprocess and Product Levels

Buldum

Mantalaris

2021

IJMS

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Engineering biological processes has become a standard approach to produce various commercially valuable chemicals, therapeutics, and biomaterials. Among these products, bacterial cellulose represents major advances to biomedical and healthcare applications. In comparison to properties of plant cellulose, bacterial cellulose (BC) shows distinctive characteristics such as a high purity, high water retention, and biocompatibility. However, low product yield and extensive cultivation times have been the main challenges in the large-scale production of BC. For decades, studies focused on optimization of cellulose production through modification of culturing strategies and conditions. With an increasing demand for BC, researchers are now exploring to improve BC production and functionality at different categories: genetic, bioprocess, and product levels as well as model driven approaches targeting each of these categories. This comprehensive review discusses the progress in BC platforms categorizing the most recent advancements under different research focuses and provides systematic understanding of the progress in BC biosynthesis. The aim of this review is to present the potential of ‘modern genetic engineering tools’ and ‘model-driven approaches’ on improving the yield of BC, altering the properties, and adding new functionality. We also provide insights for the future perspectives and potential approaches to promote BC use in biomedical applications.

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Section: Product Levelmentioning

confidence: 99%

Systematic Understanding of Recent Developments in Bacterial Cellulose Biosynthesis at Genetic, Bioprocess and Product Levels

Buldum

Mantalaris

2021

IJMS

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“…: Tonouchi et al 1995Tonouchi et al 1998b;Tonouchi et al 1998a;Nakai et al 1998;Nakai et al 1999;Nakai et al 2002;Ishida et al 2002;Nakai et al 2013 e pTA99 / pTI99 ref. : Fujiwara et al 1992;Hu et al 2010;Sunagawa et al 2013;Fang et al 2015;Jacek et al 2018;Jacek et al 2019 f pCM62 ref. : Deeraksa et al 2006;Masud et al 2010;Soemphol et al 2011;Theeragool et al 2018;Yakushi et al 2018a;Phathanathavorn et al 2019 g pSEVA ref.…”

Section: Other Expression Plasmids Recently Used In Gluconobactermentioning

confidence: 99%

“…It enabled curdlan (β-1,3-glucan) to be synthesized in K. xylinus simultaneously with cellulose nanofibers in vivo for biopreparation of nanocomposites. Production of bacterial cellulose with altered and advantageous properties could also be obtained without chemical modifications solely through altering the tight spatial organization of the cellulose fibers using a non-motile cellulose-producing K. hansenii with increased cell length and the ability to move on the surface of the medium (Jacek et al 2019;Jacek et al 2018). Therefore, the motAB genes encoding motor and stator proteins essential for flagellum rotation in numerous bacterial species were expressed either as an operon, or alone, or each gene as a translational fusion with gfp using the pTI99 vector backbone.…”

Section: Puf106 Pta99 and Pti99-a First Major Expression Plasmid Lineagementioning

confidence: 99%

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On the way toward regulatable expression systems in acetic acid bacteria: target gene expression and use cases

Fricke

Klemm

Bott

et al. 2021

Appl Microbiol Biotechnol

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Acetic acid bacteria (AAB) are valuable biocatalysts for which there is growing interest in understanding their basics including physiology and biochemistry. This is accompanied by growing demands for metabolic engineering of AAB to take advantage of their properties and to improve their biomanufacturing efficiencies. Controlled expression of target genes is key to fundamental and applied microbiological research. In order to get an overview of expression systems and their applications in AAB, we carried out a comprehensive literature search using the Web of Science Core Collection database. The Acetobacteraceae family currently comprises 49 genera. We found overall 6097 publications related to one or more AAB genera since 1973, when the first successful recombinant DNA experiments in Escherichia coli have been published. The use of plasmids in AAB began in 1985 and till today was reported for only nine out of the 49 AAB genera currently described. We found at least five major expression plasmid lineages and a multitude of further expression plasmids, almost all enabling only constitutive target gene expression. Only recently, two regulatable expression systems became available for AAB, an N-acyl homoserine lactone (AHL)-inducible system for Komagataeibacter rhaeticus and an l-arabinose-inducible system for Gluconobacter oxydans. Thus, after 35 years of constitutive target gene expression in AAB, we now have the first regulatable expression systems for AAB in hand and further regulatable expression systems for AAB can be expected. Key points • Literature search revealed developments and usage of expression systems in AAB. • Only recently 2 regulatable plasmid systems became available for only 2 AAB genera. • Further regulatable expression systems for AAB are in sight.

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“…Going beyond the operon coding for cellulose synthase, since more and more genomes of cellulose-producing strains are published and analysed, the scientists have been investigating the roles of genes responsible for general cellular behaviour. One example of such approach is the study on the motA and motB genes which build up a proton pump and which are probably involved in cell motility (Jacek et al 2018 , 2019b , 2019c ). Jacek et al subjected K. hansenii ATCC 53582 to disruption and complementation of one or both of the mentioned genes, whereas in K. hansenii ATCC 23769 induced motA and motB genes overexpression.…”

Section: Introductionmentioning

confidence: 99%

Towards control of cellulose biosynthesis by Komagataeibacter using systems-level and strain engineering strategies: current progress and perspectives

Ryngajłło

Jędrzejczak-Krzepkowska

Kubiak

et al. 2020

Appl Microbiol Biotechnol

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The strains of the Komagataeibacter genus have been shown to be the most efficient bacterial nanocellulose producers. Although exploited for many decades, the studies of these species focused mainly on the optimisation of cellulose synthesis process through modification of culturing conditions in the industrially relevant settings. Molecular physiology of Komagataeibacter was poorly understood and only a few studies explored genetic engineering as a strategy for strain improvement. Only since recently the systemic information of the Komagataeibacter species has been accumulating in the form of omics datasets representing sequenced genomes, transcriptomes, proteomes and metabolomes. Genetic analyses of the mutants generated in the untargeted strain modification studies have drawn attention to other important proteins, beyond those of the core catalytic machinery of the cellulose synthase complex. Recently, modern molecular and synthetic biology tools have been developed which showed the potential for improving targeted strain engineering. Taking the advantage of the gathered knowledge should allow for better understanding of the genotype-phenotype relationship which is necessary for robust modelling of metabolism as well as selection and testing of new molecular engineering targets. In this review, we discuss the current progress in the area of Komagataeibacter systems biology and its impact on the research aimed at scaled-up cellulose synthesis as well as BNC functionalisation. Key points • The accumulated omics datasets advanced the systemic understanding of Komagataeibacter physiology at the molecular level. • Untargeted and targeted strain modification approaches have been applied to improve nanocellulose yield and properties. • The development of modern molecular and synthetic biology tools presents a potential for enhancing targeted strain engineering. • The accumulating omic information should improve modelling of Komagataeibacter's metabolism as well as selection and testing of new molecular engineering targets.

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Structural changes of bacterial nanocellulose pellicles induced by genetic modification of Komagataeibacter hansenii ATCC 23769

Cited by 31 publications

References 83 publications

Systematic Understanding of Recent Developments in Bacterial Cellulose Biosynthesis at Genetic, Bioprocess and Product Levels

Systematic Understanding of Recent Developments in Bacterial Cellulose Biosynthesis at Genetic, Bioprocess and Product Levels

On the way toward regulatable expression systems in acetic acid bacteria: target gene expression and use cases

Towards control of cellulose biosynthesis by Komagataeibacter using systems-level and strain engineering strategies: current progress and perspectives

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