Structural Changes of Erythrocyte Surface Glycoconjugates after Treatment with Medicinal Mushrooms

Abstract

Under conditions of chronic hyperglycemia there is dysregulation of ion homeostasis, violation of redox metabolism and functioning of membrane enzymes, as well as changes in the structural and functional states of erythrocyte membranes. As a result, the aggregation ability of erythrocytes increased and their deformability decreased. These changes lead to complications to microcirculation blood flow and provoke the development of vascular complications caused by diabetes mellitus. This study investigated the effect of the medicinal mushrooms Agaricus brasiliensis and Ganoderma lucidum on the structure of carbohydrate determinants of surface membrane glycoconjugates of rat peripheral blood erythrocytes under both normal conditions and streptozotocin-induced diabetes mellitus. The research was carried out using Wistar outbred white rats. Diabetes was induced by streptozotocin intraperitoneally injected once at a dose of 50 mg/kg body weight. The mushroom preparations were orally administered at a dose of 1 g/kg for 14 days. The treatment of diabetic rats by submerged culture mycelium powder restored the physiological balance between sialylation and desialylation processes, renewed the membrane surface charge of red blood cells, normalized aggregation properties, and caused the structural recovery of oligosaccharide chains of erythrocyte membrane surface glycoconjugates. The discovered changes show an improvement in the erythrocyte functional state and rejuvenation of their population caused by biologically active compounds of the studied medicinal mushrooms.

Lentinus edodes Exposure before and after Fetus Implantation: Materno-Fetal Development in Rats with Gestational Diabetes Mellitus

“…DM-STZ can induce important hematological alterations in both white and red blood cells [41,42]. DM-STZ can also increase the number of platelets, inducing an increase in platelet aggregation [43].…”

Chaga mushroom (Inonotus obliquus) polysaccharides exhibit genoprotective effects in UVB-exposed embryonic zebrafish (Danio rerio) through coordinated expression of DNA repair genes

“…In addition, zebrafish DNA is amenable to genetic manipulation using morpholinos, shRNA, or Crisprs [ 16 ]. UV-induced zebrafish models have also been developed to study the expression of DNA repair genes and tumor suppressor genes regulation [ 17 , 18 , 19 ]. Furthermore, transparent zebrafish casper mutants have been consistently used for tumor-related and toxicological studies for assessing the phenotypic manifestations during development [ 20 ].…”

Genoprotective effects of Chaga mushroom (Inonotus obliquus) polysaccharides in UVB-exposed embryonic zebrafish (Danio rerio) through coordinated expression of DNA repair genes

“…Mushrooms possessing medicinal values, such as Ganoderma, Chaga, and many others are currently being explored significantly because of their tremendous benefits without inducing any undesirable effects, besides possessing beneficial attributes such as anti-inflammatory, anti-oxidant, and anti-tumor [12–17]. Given the wide benefits of Chaga mushroom, the effects of Chaga mushroom on the genotoxicity profile of an organism will be intriguing to explore.…”

“…Zebrafish has been used as in vivo model organism for testing a plethora of exogenous agents comprising drugs, nanoparticles, organic and inorganic compounds, besides being exposed to different stress environments. UV-induced zebrafish models have also been developed to study the expression of DNA repair genes and tumor suppressor genes regulation [15–17]. Furthermore, transparent zebrafish casper mutants have been consistently used for tumour-related and toxicological studies for assessing the phenotypic manifestations during development [18].…”